Physical studies of hemocyanins. V. Characterization and subunit structure of the hemocyanin of Cancer magister

Ellerton, H. David; Carpenter, Donald E.; Holde, Kensal E. van

doi:10.1021/bi00813a002

Cited by 82 publications

(27 citation statements)

References 15 publications

(10 reference statements)

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“…The Bio-Gel column was calibrated with crab (Cancer magister) haemocyanin (mol.wt. 9.0 x 105 and 4.5 x 105) (Ellerton et al, 1970), and bovine heart lactate dehydrogenase (mol.wt. 150000) (Sigma).…”

Section: Molecular-weight Analysesmentioning

confidence: 99%

The quarternary structure of an unusual high-molecular-weight intracellular haemoglobin from the bivalve mollusc Barbatia reeveana

Grinich

Terwilliger

1980

Biochemical Journal

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The arcid clam Barbatia reeveana contains an intracellular haemoglobin with an unusual structure. First, compared with other intracellular haemoglobins, it is extremely large, with a mol.wt. of 430000 and an s20,w of 13.6S. A minor component (mol.wt. = 220000; s2O,w = 9.7S) is also present as a probable dissociation product of the major component. Secondly, this haemoglobin has an unusual subunit structure. It contains 1 mol of haem per 16000 g of protein, in common with most other haemoglobins. However, the smallest polypeptide that could be obtained after treatment with sodium dodecyl sulphate or 6 M-guanidine with reducing agent has a mol.wt. of 32000-37000. Digestion of the haemoglobin with the proteinase subtilisin produces both 57000-and 30000-mol.wt. aggregates that contain 1 mol of haem per 16000 g of protein and that can be dissociated into 16 500-mol.wt. polypeptides by treatment with sodium dodecyl sulphate. The intact polymer shows slight co-operativity (h = 1.7), lacks a Bohr effect between pH 7 and 8, and has a low oxygen affinity [P50 = 4.8 kPa (36mmHg) at 200C] relative to other haemoglobins. The 30000-mol.wt. aggregate obtained by digestion of the polymer binds oxygen reversibly with an affinity greater than that of the polymer, but with some co-operativity (h = 1.7). These results are consistent with the hypothesis that the subunits of this unusually large intracellular haemoglobin are 32000-mol.wt. polypeptides that in turn are composed of two covalently linked haem-containing oxygen-binding domains. This is the first report of an intracellular haemoglobin with such a structure.Most intracellular haemoglobins of both vertebrates and invertebrates consist of haem-containing polypeptides with mol

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Section: Molecular-weight Analysesmentioning

confidence: 99%

The quarternary structure of an unusual high-molecular-weight intracellular haemoglobin from the bivalve mollusc Barbatia reeveana

Grinich

Terwilliger

1980

Biochemical Journal

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“…The 75-kDa subunits self-assemble into hexamers or multiples of hexamers. In the hemolymph of the Dungeness crab (Cancer magister), 2-hexamer 25 S Hc and 1-hexamer 16 S Hc occur (5).…”

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confidence: 99%

Developmental Changes in Hemocyanin Expression in the Dungeness Crab, Cancer magister

Durstewitz

Terwilliger

1997

Journal of Biological Chemistry

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“…The most common aggregation states are hexamers and dodecamers, particularly amongst the crustaceans (reviewed by Herskovits, 1988 andMarkl eta!., 1979). In a number of cases a single organism contains more than one form of hemocyanin: the hemolymph contains primarily dodecamers with a minor component of hexamers in Cancer magister (Ellerton et at., 1970), Homarus gammarus, Cancer pagurus (Markl et a/.,1979), and Callinectes sapidus .…”

Section: Structure Of Hemocyaninmentioning

confidence: 99%

“…In this species the major hemolymph form of hemocyanin is the dodecamer but some hexamer is also present (Ellerton et al, 1970). Callianiassa hemolymph normally contains 24 subunit and hexamer forms, but the hexamer form is incompetent to associate to a higher state.…”

Section: Structure Of Hemocyaninmentioning

confidence: 99%

“…The main structural consequence of pH changes on aggregation state of pH changes is denaturation at a threshold pH, usually around 10 for crustaceans such as H. americanus (Pickett eta!. , 1966), Cancer magister (Ellerton et al, 1970), and Panulirus (Makino and Kimuro, 1988). At lower pHs, previously dissociated material often reassociates to the hexameric level (Makino and Kimuro, 1988).…”

Section: Coope Rativity Cooperativitymentioning

confidence: 99%

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Developmental changes in the structure and function of lobster hemocyanin

Olson¹

1991

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Physical studies of hemocyanins. V. Characterization and subunit structure of the hemocyanin of Cancer magister

Cited by 82 publications

References 15 publications

The quarternary structure of an unusual high-molecular-weight intracellular haemoglobin from the bivalve mollusc Barbatia reeveana

The quarternary structure of an unusual high-molecular-weight intracellular haemoglobin from the bivalve mollusc Barbatia reeveana

Developmental Changes in Hemocyanin Expression in the Dungeness Crab, Cancer magister

Developmental changes in the structure and function of lobster hemocyanin

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