Phylogenetic relationships of Puccinia horiana and other rust pathogens of Chrysanthemum×morifolium based on rDNA ITS sequence analysis

Alaei, Hossein; Backer, Mathias De; Nuytinck, Jorinde; Maes, Martine; Höfte, Monica; Heungens, Kurt

doi:10.1016/j.mycres.2009.02.003

Cited by 46 publications

(25 citation statements)

References 53 publications

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“…Thus, in powdery mildew research, ITS sequences should be used as species or sub-species barcodes only if determined in this way, especially when closely related taxa are to be distinguished from each other. In rust fungi (the Uredinales), for example, cloning before sequencing the ITS region has already become a general protocol (Rossman 2007) because variations in the ITS sequences of individual samples were shown in many species (e.g., Alaei et al 2009). …”

Section: Resultsmentioning

confidence: 99%

Variation in the nrDNA ITS sequences of some powdery mildew species: do routine molecular identification procedures hide valuable information?

2011

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During the past years, nrDNA ITS sequences have supported the identification of many powdery mildew fungi because comprehensive analyses showed that differences in these sequences have always correlated with the delimitation of different species and formae speciales of the Erysiphales. Published data, obtained using direct sequencing of the PCR products, suggested that even one to five nucleotide differences in the ITS sequences delimit different, albeit closely related, species, and/or indicate differences in host range patterns. Here we show that such differences in the ITS sequences can be detected even in a single sample of a powdery mildew fungus. We sequenced the ITS region in 17 samples, representing six powdery mildew species, both directly and after cloning the PCR products. Among these, samples of O. longipes exhibited two or three, samples of O. neolycopersici three or four, those of an Oidium sp. from Chelidonium majus up to seven, and a sample of another Oidium sp. from Passiflora caerulea two different ITS types determined after cloning. No ITS nucleotide polymorphisms were found in samples of O. lycopersici and Erysiphe aquilegiae. This suggests that some powdery mildew taxa are more variable at the ITS level than others. Thus, although the ITS sequences determined by direct sequencing represent robust data useful in delimitation and phylogenetic analysis of distinct species of the Erysiphales, these need to be used with precaution, and preferably determined after cloning, especially when dealing with closely related taxa at species and sub-species levels. With this method a hitherto undetected genetic diversity of powdery mildews can be revealed.

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Section: Resultsmentioning

confidence: 99%

Variation in the nrDNA ITS sequences of some powdery mildew species: do routine molecular identification procedures hide valuable information?

2011

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“…Diseased plant material was used to inoculate fresh cuttings of the susceptible cvs Medonia, Taliedo or cv. 29 (see "Cultivars" section) as described by Alaei et al (2009). Single pustule cultures of each isolate were established before conducting pathotype tests.…”

Section: Fungal Isolatesmentioning

confidence: 99%

“…Single pustule cultures of each isolate were established before conducting pathotype tests. All the isolates were maintained by tri-weekly transfer onto fresh and rust-free cuttings of cvs Medonia and Taliedo as described by Alaei et al (2009), except the Colombian isolates, which were maintained on cv. 29, but using the same transfer schedule.…”

Section: Fungal Isolatesmentioning

confidence: 99%

“…Given these criteria and the need for leaf wetness and a high humidity for successful infection, we developed an assay that could be conducted on relatively small plants inside closed plastic containers, several of which should easily fit in a controlled environment such as a growth chamber. Expanding on previously-described inoculation methods (Firman and Martin 1968;Yamaguchi 1981;Takatsu et al 2000;Alaei et al 2009), different inoculation techniques (suspending infected leaves from the lid of the container versus spraying of macerated leaves over the plants), different ways of suspending leaves (with tape versus with agar), . Glass plates cut to size were used as covers during inoculum production.…”

Section: Bioassaymentioning

confidence: 99%

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Identification and characterization of pathotypes in Puccinia horiana, a rust pathogen of Chrysanthemum x morifolium

Backer¹,

Alaei

Bockstaele

et al. 2011

Eur J Plant Pathol

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Puccinia horiana is the causal agent of chrysanthemum white rust or Japanese rust. This microcyclic autoecious rust has a quarantine status and can cause major damage in the commercial production of Chrysanthemum x morifolium. Given the international and often trans-continental production of planting material and cut flowers of chrysanthemum and the decreasing availability of registered fungicides in specific regions, breeding for resistance against P. horiana will gain importance and will need to involve the appropriate resistance genes for the pathotypes that may be present. As pathotypes have not been well characterized in this system, the main objective was to build an international collection of isolates and screen these on a large collection of cultivars to identify different pathotypes. Using a robust and high throughput bioassay, we tested 36 selected cultivars with 22 individual single-pustule isolates of P. horiana. The isolates originated from three different continents over 4 different collection years and included some isolates from cultivars previously reported as resistant. In most cases the bioassays resulted in a clear scoring of interaction phenotypes as susceptible or resistant, while in several cases consistent intermediate phenotypes were found, often on specific cultivars. Twenty-four of the cultivars gave a differential interaction phenotype profile. All isolates produced a unique profile, infecting a minimum of 4 and a maximum of 19 differential cultivars. Based on the Person analysis of these profiles, this pathosystem contains at least seven resistance genes (and seven avirulence genes), demonstrating the highly complex race structure in this pathosystem.

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“…This provides abundant genetic information for research in mycology and fungal identification (Agarwal et al, 2008). Along with the development of molecular biology techniques, ITS sequence analysis has been widely used in taxonomy and identification of fungal species because of its good stability and sequencing convenience (Li et al, 2008;Geml et al, 2009;Alaei et al, 2009;Tedersoo et al 2009). …”

Section: Introductionmentioning

confidence: 99%

Separation and Identification of Two Fungal Strains in Stored Maize

Yue¹,

McLandsborough²,

Zou³

et al. 2017

American Journal of Biochemistry and Biotechnology

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Abstract:The microbiological spoilage of stored grain is important for human health and economic loss. Two major predominant grain spoilage fungi (M15 and M16) were isolated from maize from a granary in China. Morphological observation of the fungal isolates, analysis of their ITS sequences and construction of their phylogenetic trees were conducted on these two strains, which were identified as Fusarium solani (M15) and Aspergillus sclerotiorum (M16). This method was shown to be a feasible, accurate and convenient way to identify fungi in stored grain.

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Phylogenetic relationships of Puccinia horiana and other rust pathogens of Chrysanthemum×morifolium based on rDNA ITS sequence analysis

Cited by 46 publications

References 53 publications

Variation in the nrDNA ITS sequences of some powdery mildew species: do routine molecular identification procedures hide valuable information?

Variation in the nrDNA ITS sequences of some powdery mildew species: do routine molecular identification procedures hide valuable information?

Identification and characterization of pathotypes in Puccinia horiana, a rust pathogen of Chrysanthemum x morifolium

Separation and Identification of Two Fungal Strains in Stored Maize

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