“…Molecular phylogeny of guarani group supports the monophyly only for the guarani subgroup ( D. subbadia and D. ornatifrons ) while the guarani group is not a monophyletic group ( Robe et al 2002 , 2005 , Izumitani et al 2016 ). In two molecular studies has been proposed a tripunctata lineage cluster which includes species of guarani ( D. ornatifrons , D. griseolineata , and D. maculifrons ), tripunctata , and cardini groups ( Robe et al 2002 , Yotoko et al 2003 ). Also, a molecular phylogeny of tripunctata radiation, based on mitocondrial genes, suggested the existence of four clades, one of them is the clade tripunctata-guaramunu lineage conformed by the species D. griseolineata , D. maculifrons ( guarani group), D. frotapessoai ( Vilela and Bachli 1990 ), and D. paramediostriata ( Townsend and Wheeler 1955 ) (karyotype 2 n = 12; 5R, 1D) ( tripunctata group) ( Hatadani et al 2009 ).…”