Karyotype Description of Two Andean Species of the guarani Group of Drosophila (Díptera: Drosophilidae) and Cytological Notes

Abstract

The guarani group of Drosophila genus (Diptera: Drosophilidae) is formed by 24 species however the relationship of these species is not clear. In the present study are described the karyotypes of Drosophila sachapuyu Peñafiel and Rafael, 2018 and Drosophila zamorana Peñafiel and Rafael, 2018, two Andean species members of the guarani group. Mitotic chromosomes from cerebral ganglia of third stand larval were obtained by thermal shock and cell suspension techniques. The karyotype of D. sachapuyu, presents 2n = 10 (4R, 1V; X = R, Y = R) while D. zamorana exhibits karyotype 2n = 12 (5R, 1V; X = V, Y = R).

Metaphase chromosomes of five Neotropical species of the genus Drosophila (Diptera, Drosophilidae)

“…1A, B ). A similar 2n = 10 karyotype was reported in other species of this group: D. guaraja King, 1947 ( King 1947 ), D. butantan ( Ratcov et al 2017 ) and D. sachapuyu ( Vela and Villavicencio 2021 ). The karyotype of D. valenteae is 2n = 8 (Fig.…”

“…Most of the available cytological data about Neotropical species of Drosophila were reported in the past century ( Metz and Moses 1923 ; Patterson and Wheeler 1942 ; Wharton 1943 ; Burla et al 1949 ; Clayton and Wasserman 1957 ; Clayton and Wheeler 1975 ). In the most recent cytological studies of Neotropical species of Drosophila karyotypes of ten species from four sibling species groups have been described: D. chorlavi Céspedes et Rafael, 2012, D. mesophragmatica Duda, 1927 and D. rucux Céspedes et Rafael, 2012 from the D. mesophragmatica group ( Mafla 2012 ), D. butantan Ratcov, Vilela et Goñi, 2017, D. sachapuyu Peñafiel-Vinueza et Rafael, 2018, and D. zamorana Peñafiel-Vinueza et Rafael, 2018 from the D. guarani group ( Ratcov et al 2017 ; Vela and Villavicencio 2021 ), D. huancavilcae Rafael et Arcos, 1989, D. inca Dobzhansky et Pavan, 1943, and D. yangana Rafael et Vela,;2003 from the D. repleta group ( Mafla 2005 , 2008 ), and D. montevidensis Goñi et Vilela, 2016 from the D. tripunctata group ( Goñi and Vilela 2016 ).…”