Phylogenetic Distribution of Extraintestinal Virulence‐Associated Traits in<i>Escherichia coli</i>

Johnson, James R.; Delavari, Parissa; Kuskowski, Michael A.; Stell, Adam L.

doi:10.1086/317656

Cited by 342 publications

(345 citation statements)

References 42 publications

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“…However, this distribution is very different from that observed in the entire E. coli Reference Collection (ECOR collection) where only 3 % of the strains are ibeA + (Johnson et al, 2001a). The reason for this might be that strains from the ECOR collection are mainly of human faecal origin and that ibeA is less likely to be found in such isolates (Bingen et al, 1997;Johnson et al, 2001a). Our analysis also allowed comparison of the prevalence of ibeA with the serogroup of strains.…”

Section: Discussionmentioning

confidence: 73%

“…The prevalence of ibeA in APEC strains (26 %) is not very different from the frequencies observed in isolates associated with neonatal meningitis (40-33 %) or vaginal isolates (32 %) (Johnson et al, 2001a;Obata-Yasuoka et al, 2002). However, this distribution is very different from that observed in the entire E. coli Reference Collection (ECOR collection) where only 3 % of the strains are ibeA + (Johnson et al, 2001a). The reason for this might be that strains from the ECOR collection are mainly of human faecal origin and that ibeA is less likely to be found in such isolates (Bingen et al, 1997;Johnson et al, 2001a).…”

Section: Discussionmentioning

confidence: 76%

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ibeA, a virulence factor of avian pathogenic Escherichia coli

et al. 2005

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The presence of ibeA, a gene encoding a known virulence factor of Escherichia coli strains responsible for neonatal meningitis in humans, was investigated in the genome of 213 avian pathogenic E. coli (APEC) strains and 55 non-pathogenic E. coli strains of avian origin. Fifty-three strains were found to be ibeA + , all of which belonged to the APEC group. The ibeA gene is therefore positively linked to the pathogenicity of strains (P<0?0001). Analysis of the serogroup of strains revealed a positive association of ibeA with serogroups O18, O88 and O2. On the contrary, only 1/59 O78 strains are ibeA + , indicating a negative association of ibeA with this serogroup (P<0?0001). The role of ibeA in the virulence of the APEC strain BEN 2908 was investigated by constructing an ibeA mutant. Challenge assays on 3-week-old chickens showed a reduced virulence for the ibeA mutant. Furthermore, the APEC strain BEN 2908 was able to invade brain microvascular epithelial cells, this invasion being significantly reduced upon inactivation of ibeA. Altogether, these results suggest a role of ibeA in the pathogenicity of some APEC strains and confirm the close relationship between APEC and other human extraintestinal pathogenic E. coli isolates.

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Section: Discussionmentioning

confidence: 73%

Section: Discussionmentioning

confidence: 76%

ibeA, a virulence factor of avian pathogenic Escherichia coli

et al. 2005

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“…Of the four E. coli phylotype groups (A, B1, B2, D), phylotype B2 has the highest presence of extra-intestinal virulence factors and has been suggested to have the greatest resistance to antibiotics (e.g., Johnson et al 2001;Skurnik et al 2005). Typically phylotype B2 is dominant in E. coli strains isolated from human and omnivorous terrestrial Australian mammals, while B1 is the most abundant phylotype in carnivorous species (Gordon and Cowling 2003;Escobar-Páramo et al 2006).…”

Section: Discussionmentioning

confidence: 99%

MOLECULAR DETECTION OF ANTIBIOTIC-RESISTANCE DETERMINANTS INESCHERICHIA COLIISOLATED FROM THE ENDANGERED AUSTRALIAN SEA LION (NEOPHOCA CINEREA)

Delport

Harcourt

Beaumont

et al. 2015

Journal of Wildlife Diseases

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ABSTRACT:Greater interaction between humans and wildlife populations poses significant risks of anthropogenic impact to natural ecosystems, especially in the marine environment. Understanding the spread of microorganisms at the marine interface is therefore important if we are to mitigate adverse effects on marine wildlife. We investigated the establishment of Escherichia coli in the endangered Australian sea lion (Neophoca cinerea) by comparing fecal isolation from wild and captive sea lion populations. Fecal samples were collected from wild colonies March 2009-September 2010 and from captive individuals March 2011-May 2013. Using molecular screening, we assigned a phylotype to E. coli isolates and determined the presence of integrons, mobile genetic elements that capture gene cassettes conferring resistance to antimicrobial agents common in fecal coliforms. Group B2 was the most abundant phylotype in all E. coli isolates (n537), with groups A, B1, and D also identified. Integrons were not observed in E. coli (n521) isolated from wild sea lions, but were identified in E. coli from captive animals (n516), from which class I integrases were detected in eight isolates. Sequencing of gene cassette arrays identified genes conferring resistance to streptomycin-spectinomycin (aadA1) and trimethoprim (dfrA17, dfrB4). Class II integrases were not detected in the E. coli isolates. The frequent detection in captive sea lions of E. coli with resistance genes commonly identified in human clinical cases suggests that conditions experienced in captivity may contribute to establishment. Identification of antibiotic resistance in the microbiota of Australian sea lions provides crucial information for disease management. Our data will inform conservation management strategies and provide a mechanism to monitor microorganism dissemination to sensitive pinniped populations.

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“…Several studies have investigated the similarities of UPEC and APEC in their serogroups, virulence genotypes and assignments to phylogenetic groups (Johnson et al, 1998(Johnson et al, , 2001aKaper et al, 2004;Mokady et al, 2005;Johnson et al, 2006a;Moulin-Schouleur et al, 2006). It has been proposed that poultry may be a candidate vehicle for E. coli capable of causing human urinary tract disease, based on research showing transmission of avian E. coli from poultry to humans or similarities between avian E. coli and UPEC (Levy et al, 1976;Linton et al, 1977;Ojeniyi, 1989;van den Bogaard et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Comparison of virulence factors and expression of specific genes between uropathogenic Escherichia coli and avian pathogenic E. coli in a murine urinary tract infection model and a chicken challenge model

et al. 2009

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Avian pathogenic Escherichia coli (APEC) and uropathogenic E. coli (UPEC) establish infections in extraintestinal habitats of different hosts. As the diversity, epidemiological sources and evolutionary origins of extraintestinal pathogenic E. coli (ExPEC) are so far only partially defined, in the present study,100 APEC isolates and 202 UPEC isolates were compared by their content of virulence genes and phylogenetic groups. The two groups showed substantial overlap in terms of their serogroups, phylogenetic groups and virulence genotypes, including their possession of certain genes associated with large transmissible plasmids of APEC. In a chicken challenge model, both UPEC U17 and APEC E058 had similar LD 50 , demonstrating that UPEC U17 had the potential to cause significant disease in poultry. To gain further information about the similarities between UPEC and APEC, the in vivo expression of 152 specific genes of UPEC U17 and APEC E058 in both a murine urinary tract infection (UTI) model and a chicken challenge model was compared with that of these strains grown statically to exponential phase in rich medium. It was found that in the same model (murine UTI or chicken challenge), various genes of UPEC U17 and APEC E058 showed a similar tendency of expression. Several iron-related genes were upregulated in the UTI model and/or chicken challenge model, indicating that iron acquisition is important for E. coli to survive in blood or the urinary tract. Based on these results, the potential for APEC to act as human UPEC or as a reservoir of virulence genes for UPEC should be considered. Further, this study compared the transcriptional profile of virulence genes among APEC and UPEC in vivo.

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Phylogenetic Distribution of Extraintestinal Virulence‐Associated Traits inEscherichia coli

Cited by 342 publications

References 42 publications

ibeA, a virulence factor of avian pathogenic Escherichia coli

ibeA, a virulence factor of avian pathogenic Escherichia coli

MOLECULAR DETECTION OF ANTIBIOTIC-RESISTANCE DETERMINANTS INESCHERICHIA COLIISOLATED FROM THE ENDANGERED AUSTRALIAN SEA LION (NEOPHOCA CINEREA)

Comparison of virulence factors and expression of specific genes between uropathogenic Escherichia coli and avian pathogenic E. coli in a murine urinary tract infection model and a chicken challenge model

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