Phylogenetic analysis of the ballistoconidium-forming yeast genus Sporobolomyces based on 18S rDNA sequences.

Abstract

The 18S rDNA nucleotide sequences of 25 Sporobolomyces species and five Sporidiobolus species were determined. Those of Sporobolomyces dimmenae JCM 8762 T , Sporobolomyces ruber JCM 6884 T , Sporobolomyces sasicola JCM 5979 T and Sporobolomyces taupoensis JCM 8770 T showed the presence of intron-like regions with lengths of 1586, 324, 322 and 293 nucleotides, respectively, which were presumed to be group I introns. A total of 63 18S rDNA nucleotide sequences was analysed, including 33 published reference sequences. Sporobolomyces species and the other basidiomycetes species were distributed throughout the phylogenetic tree. The resulting phylogeny indicated that Sporobolomyces is polyphyletic. Sporobolomyces species were mainly divided into four groups within the Urediniomycetes. The groups are designated as the Sporidiales, Agaricostilbum/Bensingtonia, Erythrobasidium and subbrunneus clusters. The last group, comprising four species, Sporobolomyces coprosmicola, Sporobolomyces dimmenae , Sporobolomyces linderae and Sporobolomyces subbrunneus, forms a new and distinct cluster in the phylogenetic tree in this study.

Phylogenetic relationship within the Erythrobasidium clade: Molecular phylogenies, secondary structure, and intron positions inferred from partial sequences of ribosomal RNA and elongation factor-1α genes

“…Formerly published phylogenies for the Erythrobasidium clade (Bai et al, 2001;Fell et al, 1995Fell et al, , 2000Hamamoto and Nakase, 2000;Hamamoto et al, 2002;Nagahama et al, 2001;Scorzetti et al, 2002) all agreed that the members of this clade were divided into the same subgroups, with the exception of S. gracilis. Based on this agreement, we defined the six subgroups as Aurantiaca, Bannoa, Erythrobasidium, Marina, Occultifur, and Sakaguchia (Table 1).…”

“…Only the subgroup Erythrobasidium tended not to be monophyletic in its 18S rDNA or ITS sequences, but the other subgroups were robust through all the previous phylogenetic analyses. Sporobolomyces gracilis was clustered within subgroup Aurantiaca based on 18S rDNA analysis (Bai et al, 2001;Hamamoto and Nakase, 2000;Hamamoto et al, 2002), whereas it was within subgroup Marina based on ITS or 26S rDNA analysis (Fell et al, 2000;Nagahama et al, 2001;Scorzetti et al, 2002). Rhodotorula mucilaginosa was selected as a common outgroup for all phylogenetic analyses.…”

Emendation of Dioszegia with redescription of Dioszegia hungarica and two new combinations, Dioszegia aurantiaca and Dioszegia crocea.

“…ballistoconidium-forming yeasts (Fell et al, 2000;Hamamoto and Nakase, 2000;Takashima and Nakase, 1999). During phylogenetic analyses, we found that hymenomycetous yeast species were phylogenetically divided into several lineages and that these orange-colored species occurred in the Cryptococcus luteolus lineage with other Bullera and Cryptococcus species (Takashima and Nakase, 1999).…”

Bensingtonia changbaiensis sp. nov. and Bensingtonia sorbi sp. nov., novel ballistoconidium-forming yeast species from plant leaves

“…In order to confirm the phylogenetic positions of the two groups, 18S rDNA sequences of the representative strains CB 286 T and CB 346 T were determined and aligned with those of reference taxa, including all described Bensingtonia species and related urediniomycetous yeast taxa. The two strains clustered together with five Bensingtonia and one Kondoa species in the Agaricostilbum/Bensingtonia lineage (Hamamoto & Nakase, 2000) with 100 % bootstrap support. CB 286 T was located with Bensingtonia miscanthi, whereas CB 346 T showed a close relationship to B. yuccicola.…”

“…L-Arabinose 2 2 2 + 2 2 L 2 W 2 2 2 2 2 Nitrate L 2 + + 2 + 2 + + 2 2 + 2 + genera, has been demonstrated by rRNA gene sequence analyses (Takashima et al, 1995;Fell et al, 2000;Hamamoto & Nakase, 2000;Scorzetti et al, 2002), redefinitions of these genera would be premature at present. Relationships among lineages and clades of these yeasts should become clearer as novel species are added to the phylogenetic trees, which is hoped to make the demarcation of genera more reliable.…”