Bensingtonia changbaiensis sp. nov. and Bensingtonia sorbi sp. nov., novel ballistoconidium-forming yeast species from plant leaves

Abstract

Six ballistoconidium-forming yeast strains that were isolated from plant leaves collected on Changbai Mountain, north-east China, were assigned to the genus Bensingtonia Ingold emend. Nakase & Boekhout due to the formation of asymmetrical ballistoconidia, cream-coloured colonies and Q-9 as the major ubiquinone. Two separate groups, representing two novel Bensingtonia species, were recognized among these yeasts by 26S rDNA D1/D2 domain, internal transcribed spacer (ITS) region and 18S rDNA sequence analyses. The names Bensingtonia changbaiensis sp. nov. (type strain, CB 346 T =AS 2.2310 T =CBS 9497 T ) and Bensingtonia sorbi sp. nov. (type strain, CB 286 T =AS 2.2303 T =CBS 9498 T ) are proposed for these two species.

Naganishia floricola sp. nov., a novel basidiomycetous yeast species isolated from flowers of Sorbaria sorbifolia

“…After purification, the PCR products were directly sequenced with the forward primers ITS1 and NL1 and the reverse primers ITS4 and NL4. The SSU sequences were obtained following methods in previous literature [3, 27, 28]. Primers for amplification of SSU were P1F and U3R.…”

“…Primers for sequencing included four forward primers: P1F, 570F, 934F and 1272F; and three reverse primers: U1R, U2R and U3R [3]. The amplification reaction for SSU was performed using the following parameters: 94 °C for 3 min, followed by 33 cycles of denaturation at 94 °C for 30 s, denaturation at 55 °C for 1 min, extension at 72 °C for 2.5 min, with a final extension at 72 °C for 10 min [27, 28]. PCR and sequencing primers for RPB1 , RPB2 and TEF1 were described previously [29].…”

Bensingtonia pseudonaganoensis sp. nov., a novel ballistoconidium-forming yeast species isolated from plant leaves

“…This strain has been deposited Physiologically, B. pseudonaganoensis sp. nov. is similar to another species from China Bensingtonia changbaiensis (Wang et al 2003). They differed in the assimilation results for melibiose, soluble starch and nitrite.…”

“…The species of the genus are scattered in different lineages or clades of basidiomycetous yeasts. Bensingtonia naganoensis is the only other previously described Bensingtonia species that clustered together with the type species of the genus, Bensingtonia ciliata, in a separate branch (Fell et al 2000;Scorzetti et al 2002;Wang et al 2003).…”

Naumovozyma baii sp. nov., an ascomycetous yeast species isolated from rotten wood in a tropical forest

“…The ITS region and 26S rRNA gene D1/D2 domain sequences were determined using the methods described by Bai et al (2002). PCR for amplification of the 18S rRNA gene and cycle sequencing using PCR products were performed as described by Wang et al (2003). The BLAST search program (Altschul et al, 1990) was used to compare the sequences generated in this study and those of other yeasts in GenBank.…”