Phylogenetic analysis of kinetoplast DNA: kDNA of Leishmania tropica in Thi-Qar province, Iraq

Flaih, Mohammed Hassan; Al-Abady, Fadhil Abbas; Hussein, Khwam R

doi:10.1016/j.cimid.2021.101696

Cited by 6 publications

(3 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Al-Obaidi et al [25] have conducted a survey based on Iraqi CDC database (2008-2015), 17,001 cases recorded in all parts Iraq. Flaih et al, [26] recorded 247 cases of CL in Thi-Qar.…”

Section: Prevalence Of CL and Geographical Distributionmentioning

confidence: 99%

Geographical Distribution of Cutaneous Leishmaniasis and Pathogenesis

Flaih¹

2022

Leishmaniasis - General Aspects of a Stigmatized Disease

View full text Add to dashboard Cite

Leishmaniasis is still considered to be a global health problem, which spreads in most countries in the world. Leishmania is an intracellular obligate protistan parasite that causes different clinical symptoms in infected humans and other animals. There are clinically different types of the disease including: visceral, cutaneous or muco-cutaneous leishmaniasis. Approximately, two million new infections occurring annually; 0.7 to 1.2 million cases are recorded with cutaneous leishmaniasis and 200,000–400,000 cases return for visceral leishmaniasis. However, Cutaneous leishmaniasis considers one of uncontrolled wobbling endemic diseases, especially in Iraq, which occurs at the skin to cause a dermal lesion. Usually, the lesion is spontaneously healed to leave a colorless depressed scar and permanent immunity.

show abstract

“…Al-Obaidi et al [25] have conducted a survey based on Iraqi CDC database (2008-2015), 17,001 cases recorded in all parts Iraq. Flaih et al, [26] recorded 247 cases of CL in Thi-Qar.…”

Section: Prevalence Of CL and Geographical Distributionmentioning

confidence: 99%

Geographical Distribution of Cutaneous Leishmaniasis and Pathogenesis

Flaih¹

2022

Leishmaniasis - General Aspects of a Stigmatized Disease

View full text Add to dashboard Cite

show abstract

“…Leishmania species are protozoa that belong to the phylum Sarcomastigophora, class Zoomastigophorea, order Kinetoplastida, and family Trypanosomatidae ( 4 ). Leishmania parasites can cause leishmaniasis, with the main hosts being vertebrates, including mainly rodents, Canidae species, and humans ( 38 ). To date, more than 88 countries in the world have reported cases of Leishmania infection, with 12 million people infected ( 39 ).…”

Section: Phylum Sarcomastigophora Class Zoomastigophoreamentioning

confidence: 99%

The State of Art of Extracellular Traps in Protozoan Infections (Review)

2021

View full text Add to dashboard Cite

Protozoan parasite infection causes severe diseases in humans and animals, leading to tremendous economic and medical pressure. Natural immunity is the first line of defence against parasitic infection. Currently, the role of natural host immunity in combatting parasitic infection is unclear, so further research on natural host immunity against parasites will provide a theoretical basis for the prevention and treatment of related parasitic diseases. Extracellular traps (ETs) are an important natural mechanism of immunity involving resistance to pathogens. When immune cells such as neutrophils and macrophages are stimulated by external pathogens, they release a fibrous network structure, consisting mainly of DNA and protein, that can capture and kill a variety of extracellular pathogenic microorganisms. In this review, we discuss the relevant recently reported data on ET formation induced by protozoan parasite infection, including the molecular mechanisms involved, and discuss the role of ETs in the occurrence and development of parasitic diseases.

show abstract

“…For the abovementioned purposes, molecular techniques are among the best options [12,13]. Several nuclear DNA markers, including cytochrome b (CytB), the internal transcribed spacer (ITS), glycoprotein 63 (gp63), and kinetoplast DNA (kDNA), have been used to identify species and study genetic diversity in Leishmania [14][15][16][17]. The last one was reported as the most sensitive option to detect the parasite DNA in low parasite load samples [18].…”

Section: Introductionmentioning

confidence: 99%

Detection and phylogenetic analysis of kinetoplast DNA of Leishmania infantum infected humans, domestic dogs and sandflies in Northwest Iran

Behniafar,

Taghipour,

Spotin

et al. 2024

PLoS ONE

View full text Add to dashboard Cite

Leishmaniasis refers to a disease with a wide range of manifestations; and there are three main forms of disease, cutaneous, mucocutaneous, and visceral. Leishmaniasis is one of the diseases with a protozoan agent which is vector-borne. Visceral leishmaniasis (VL) is the most severe form that can be fiercely life-threatening if left untreated. VL can be caused by members of Leishmania donovani complex, in Iran, Leishmania infantum is considered the primary causative agent of VL, resulting in a zoonotic form of VL. The two main goals of our work, which followed our prior sero-epidemiological and entomological survey, were to characterize and conduct a phylogenetic analysis of the Leishmania species that infect people, dogs, and sandflies. The samples were collected throughout 2017, from January to December, so blood samples were collected from humans and dogs, while sandfly samples were collected with sticky traps. DNA extracted from all seropositive samples of humans and dogs, 10% of sero-negative human samples, and all collected sandflies were subjected to kDNA-nested-PCR for tracing parasites. A total of 30 samples, including 20 human samples, 8 dog samples, and 2 sandfly samples, were found positive for the kDNA gene of L. infantum. Sequences were evaluated to study the genetic diversity among the six discovered L. infantum. Based on kDNA, the phylogenetic study of L. infantum demonstrated a high level of genetic variety and a relationship between the host, the parasite’s geographic origin, and its genetic diversity.

show abstract

Phylogenetic analysis of kinetoplast DNA: kDNA of Leishmania tropica in Thi-Qar province, Iraq

Cited by 6 publications

References 31 publications

Geographical Distribution of Cutaneous Leishmaniasis and Pathogenesis

Geographical Distribution of Cutaneous Leishmaniasis and Pathogenesis

The State of Art of Extracellular Traps in Protozoan Infections (Review)

Detection and phylogenetic analysis of kinetoplast DNA of Leishmania infantum infected humans, domestic dogs and sandflies in Northwest Iran

Contact Info

Product

Resources

About