Phylogenetic analysis of genotype VII of new castle disease virus in Indonesia

Abstract

Newcastle disease (ND) is a very contagious disease in chickens and turkeys and one of the most important diseases of poultry in the world. The infection causes sudden death with high mortality. In Indonesia, Newcastle disease in recent years showed symptoms slightly different from previous symptoms of this disease. NDV infection of genotipe VII has been reported to cause this outbreak in several commercial poultry farms in Indonesia. This study aimed to isolate and identify the recent ND virus and determine group of genotype of ND in Indonesia for the development of seed of ND vaccines expected to be more effective in the control of Newcastle disease in the field. The method used in this study includes the collection of samples from the field, ND virus isolation, RT-PCR and DNA sequencing of the F and HN genes of ND viruses. Our result showed that six isolates belong to genotype VII of ND viruses, one isolate belong to genotype VI and the other isolate belong to genotype I. The prediction of pathotypes of amino acids sequence on F gene of NDV indicated that seven isolates have motif R-R-R-K-R and R-R-Q-K-R which is a marker for pathotype for velogenic of ND viruses. The cleavage site of amino acid sequences from one isolate (RIVS isolate) has G-K-Q-G-R-L which is lentogenic pathotype of NDV. This study indicated that genotype VII viruses were predominant virus circulating in the field and we suggested the update of master seed vaccine of ND in Indonesia.

In vitro expression of the recombinant fusion protein of Newcastle disease virus from local Indonesian isolates by using a cell-free protein expression system

“…An outbreak of ND can cause a devastating effect as it causes almost 100% mortality in susceptible poultry (Alexander et al 2012). ND was also reported to have a significant economic effect on the poul try industry in Indonesia (Dharmayanti et al 2014). New castle disease is caused by avian paramyxovirus serotipe1 (APMV1) virus, which is also known as Newcastle dis ease virus or NDV (Alexander 2003).…”

“…Newcastle disease outbreak was reported in 2009 2010 in poultry farms in Indonesia, which caused death on 7080% of the total number of chickens (Dharmayanti et al 2014). Analysis on the amino acid identity of F and HN protein sequences from eight isolates NDV, which caused the outbreak indicated antigenic differences from La Sota and B1 vaccine strains (Xiao et al 2012).…”

Pathotypic and Phylogenetic Analysis of Newcastle Disease Virus Isolated from Vaccinated Chicken in West Java, Indonesia

“…In 2009 and 2010, outbreaks of ND occurred in commercial chickens in Indonesia, causing up to 70% to 80% mortality (Xiao et al 2012;OIE 2012). Recently, NDV infection of genotype VII has been reported to cause outbreaks in several commercial poultry farm in Indonesia (Xiao et al 2012;Dharmayanti et al 2014). West Java is one of the provinces in Indonesia have experienced recurrent outbreak of ND because high density of poultry population in these areas.…”

Review: Strategi Praktis Penanganan Egg Drop Syndrome pada Ayam Petelur

“…Golongan lainnya adalah vaksin inaktif merupakan vaksin yang didalamnya merupakan agen infeksi yang telah dimatikan tetapi masih mampu menstimulasi pembentukan antibody, golongan vaksin ini termasuk killed vaccine dan inactivated vaccine (Dharmayanti et al, 2014) . Kencana et al (2017) menjelaskan bahwa vaksin kombinasi inaktif ND-IB-EDS menunjukkan ayam petelur titer antibodi EDS yang tidak protektif, hanya protektif terhadap ND.…”