Phylogenetic analysis of bacterial communities in the shrimp and sea cucumber aquaculture environment in northern China by culturing and PCR–DGGE

Li, Qiufen; Zhang, Y.; Juck, David; Fortin, Nathalie; Greer, Charles W.; Tang, Qisheng

doi:10.1007/s10499-009-9316-9

Cited by 13 publications

(7 citation statements)

References 19 publications

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“…However, although the juveniles and adults of C. corteziensis and C. gigas were collected in warm water, which has a positive effect on the abundance of Vibrios, these bacteria were not found. In recent years, some authors have suggested that Vibrio is not necessarily predominant in the microbiota of healthy oysters [29,42]. We analyzed samples from healthy and depurated oysters, which may explain why we did not find Vibrios to be a component of the microbiota of C. gigas and C. corteziensis.…”

Section: Discussionmentioning

confidence: 82%

Molecular Analysis of Bacterial Microbiota Associated with Oysters (Crassostrea gigas and Crassostrea corteziensis) in Different Growth Phases at Two Cultivation Sites

et al. 2012

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Section: Discussionmentioning

confidence: 82%

Molecular Analysis of Bacterial Microbiota Associated with Oysters (Crassostrea gigas and Crassostrea corteziensis) in Different Growth Phases at Two Cultivation Sites

et al. 2012

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“…Seasonal pattern of bacterioplankton community composition has been reported for aquaculture systems 32 , river 33 , lake 34 , coastal water 35 and the open ocean 36 . However, these previous studies were mostly based on fingerprinting methods, which only include the most abundant members of the community.…”

Section: Discussionmentioning

confidence: 98%

Bacterial abundance and diversity in pond water supplied with different feeds

Qin

Hou

Deng

et al. 2016

Sci Rep

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The abundance and diversity of bacteria in two types of ponds were investigated by quantitative PCR and Illumina MiSeq sequencing. The results revealed that the abundance of bacterial 16S rRNA genes in D ponds (with grass carp fed sudan grass) was significantly lower than that in E ponds (with grass carp fed commercial feed). The microbial communities were dominated by Proteobacteria, Cyanobacteria, Bacteroidetes, and Actinobacteria in both E and D ponds, while the abundance of some genera was significantly different between the two types of ponds. Specifically, some potential pathogens such as Acinetobacter and Aeromonas were found to be significantly decreased, while some probiotics such as Comamonadaceae unclassified and Bacillales unclassified were significantly increased in D ponds. In addition, water quality of D ponds was better than that of E ponds. Temperature, dissolved oxygen and nutrients had significant influence on bacterial communities. The differences in bacterial community compositions between the two types of ponds could be partially explained by the different water conditions.

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“…Understanding bacterial diversity becomes important to improve the application of microbial products as the native bacterial community can sway the abundance and function of any microbes added to the system. The microbial composition has been studied in the water column and sediment of shrimp ponds (Li, Zhang, Juck, Fortin, Greer & Tang ; Tang, Tao, Tan, Mu, Peng, Yang, Tong & Chen ; Zhang, Sun, Liu, Xuan, Jiang, Pan, Zhang, Gong, Lu, Yu, Kumar, Hu, Cao, Xue & Gong ), freshwater crab ponds (Liu, Zhou, He, Yao & Ringo ), seawater fish ponds (Zeng, Ma, Wei, Jiao, Tang, Wu & Jian ; Pereira, Salvador, Arrojado, Silva, Santos, Cunha, Gomes & Almeida ) and lobster tanks (Bourne, Young, Webster, Payne, Salmon, Demel & Hall ; Payne, Hall, Sly & Bourne ). However, knowledge in bacterial diversity in freshwater polyculture and integrated ponds is scarce (Zhou, Wang, Tang & Dai ; Huang, Liu, Li & Wang ).…”

Section: Introductionmentioning

confidence: 99%

Bacterial composition, abundance and diversity in fish polyculture and mussel-fish integrated cultured ponds in China

et al. 2016

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Bacterial community and abiotic environmental parameters in twelve freshwater aquaculture ponds were analysed. According to the major component of stocked animals, the ponds were grouped into four types: black carp Mylopharyngodon piceus, largemouth bass Micropterus salmoides, yellow catfish Pelteobagrus fulvidraco and pearl mussel Hyriopsis cumingii ponds. Each type of pond was stocked with three species of Chinese carps (silver carp, bighead carp and gibel carp) to form a unique mode of fish polyculture or mussel–fish integrated culture. The bacterial composition was identified using 16S rDNA sequencing. Totally, 3701 and 11 150 operational taxonomic units (OTUs) were identified from the water and sediment samples respectively. The number of OTUs, abundance‐based coverage estimator, Chao1 index and Shannon diversity index were lower in the water column than in the sediment, suggesting that diversity and stability of bacterial community were higher in the sediment. In the water column, Proteobacteria, Actinobacteria and Bacteroidetes dominated at the phylum level, and 26 dominant genera were identified. In the sediment, Proteobacteria, Chloroflexi, Bacteroidetes, Acidobacteria and Nitrospirae dominated at the phylum level, and 25 dominant genera were identified. Bacterial compositions between the ponds with different aquaculture modes were similar at the phylum levels, but varied at the genus levels. The bacterial composition in the ponds was correlated with hardness, ammonia and total nitrogen in the water column. This study indicates that the type of aquaculture mode is a factor regulating the microbial community, which provides an insight towards microbial management through probiotic manipulation in pond culture.

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Phylogenetic analysis of bacterial communities in the shrimp and sea cucumber aquaculture environment in northern China by culturing and PCR–DGGE

Cited by 13 publications

References 19 publications

Molecular Analysis of Bacterial Microbiota Associated with Oysters (Crassostrea gigas and Crassostrea corteziensis) in Different Growth Phases at Two Cultivation Sites

Molecular Analysis of Bacterial Microbiota Associated with Oysters (Crassostrea gigas and Crassostrea corteziensis) in Different Growth Phases at Two Cultivation Sites

Bacterial abundance and diversity in pond water supplied with different feeds

Bacterial composition, abundance and diversity in fish polyculture and mussel-fish integrated cultured ponds in China

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