Molecular Analysis of Bacterial Microbiota Associated with Oysters (Crassostrea gigas and Crassostrea corteziensis) in Different Growth Phases at Two Cultivation Sites

Trabal, Natalia; Mazón‐Suástegui, José Manuel; Vázquez-Juárez, Ricardo; Asencio-Valle, Felipe; Morales-Bojórquez, Enrique; Romero, Jaime

doi:10.1007/s00248-012-0039-5

Cited by 91 publications

(96 citation statements)

References 65 publications

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“…Tissues of healthy marine invertebrates usually harbor species-rich microbial communities (Prieur et al, 1990;Gomez-Gil et al, 1998;Romero et al, 2002;Gomez-Gil et al, 2010;King et al, 2012;Trabal et al, 2012;Wegner et al, 2013). This also applies to the hemolymph (Olafsen et al, 1993;Garnier et al, 2007, Wendling et al, 2014-the functional analog of blood in vertebrates (Bachere et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…Many temperature-dependent disease outbreaks have been linked to various Vibrio species (Lacoste et al, 2001;Lee et al, 2001;Garnier et al, 2007;Cervino et al, 2008;Elston et al, 2008) that are commonly isolated from healthy marine animals (Prieur et al, 1990;Olafsen et al, 1993;Gomez-Gil et al, 1998;Iida et al, 2000;Vega Thurber et al, 2009;Trabal et al, 2012;Zhao et al, 2012). The effects of temperature on Vibrio spp.…”

Section: Introductionmentioning

confidence: 99%

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Hemolymph microbiome of Pacific oysters in response to temperature, temperature stress and infection

2014

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Microbiota provide their hosts with a range of beneficial services, including defense from external pathogens. However, host-associated microbial communities themselves can act as a source of opportunistic pathogens depending on the environment. Marine poikilotherms and their microbiota are strongly influenced by temperature, but experimental studies exploring how temperature affects the interactions between both parties are rare. To assess the effects of temperature, temperature stress and infection on diversity, composition and dynamics of the hemolymph microbiota of Pacific oysters (Crassostrea gigas), we conducted an experiment in a fully-crossed, three-factorial design, in which the temperature acclimated oysters (8 or 22 1C) were exposed to temperature stress and to experimental challenge with a virulent Vibrio sp. strain. We monitored oyster survival and repeatedly collected hemolymph of dead and alive animals to determine the microbiome composition by 16s rRNA gene amplicon pyrosequencing. We found that the microbial dynamics and composition of communities in healthy animals (including infection survivors) were significantly affected by temperature and temperature stress, but not by infection. The response was mediated by changes in the incidence and abundance of operational taxonomic units (OTUs) and accompanied by little change at higher taxonomic levels, indicating dynamic stability of the hemolymph microbiome. Dead and moribund oysters, on the contrary, displayed signs of community structure disruption, characterized by very low diversity and proliferation of few OTUs. We can therefore link short-term responses of host-associated microbial communities to abiotic and biotic factors and assess the potential feedback between microbiota dynamics and host survival during disease.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hemolymph microbiome of Pacific oysters in response to temperature, temperature stress and infection

2014

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“…The amplified PCR products were separated by electrophoresis in 5% polyacrylamide gels in 1× TBE (tris-borate-EDTA) buffer at 150 V for 50 min and visualized by 30 min of incubation with SYBR Green at room temperature (Molecular Probes, Eugene, OR, USA). The band sizes were estimated by comparison against 100-bp DNA ladders and excised from the gel on a UV-transilluminator using a sterile scalpel as described by Trabal et al (2012). The resulting slices were placed separately in 0.6 ml Eppendorf tube with 50 µl of nuclease-free water and incubated overnight at 4°C for elution of the DNA.…”

Section: Samplesmentioning

confidence: 99%

Technical note: use of internal transcribed spacer for ruminal yeast identification in dairy cows

Vargas‐Bello‐Pérez

Cancino‐Padilla

Romero

2016

animal

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Molecular techniques are important tools for microbiological studies in different habitats, and the internal transcribed spacer (ITS) has been proved to be useful for analyzing fungal diversity. The aim of this study was to use the ITS region to generate ruminal yeast profile and to identify ruminal yeast. DNA from ruminal digesta was extracted to amplify the ribosomal ITS region. The profile from the PCR products was visualized and the excised bands from the profile were identified as the genera Millerozyma, Pichia, Rhizomucor and Hyphopichia. Overall, the ITS resulted to be a simple, fast and sensitive approach that allowed profiling and identification of ruminal yeast that have not been previously described (Millerozyma and Hyphopichia) in the rumen microbial community.

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“…Therefore, the microbiological quality of oysters is closely related to the sanitary conditions of water, and can be seriously affected by untreated effluents from urban or rural areas (Souza et al, 2012). The anthropic impact and water pollution destabilize the microorganism population (La Valley et al, 2009), which is essential for the maintenance of health by inhibiting the pathogen colonization in oysters (Trabal et al, 2012). The misbalance of the microbial communities can negatively influence the aquaculture activities, leading to a possible increase in human pathogens (Forrest et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Microbiological quality and bacterial diversity of the tropical oyster Crassostrea rhizophorae in a monitored farming system and from natural stocks

et al. 2015

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ABSTRACT. Microbiological evaluation is one of the most important parameters for analyzing the viability of an oyster farming system, which addresses public health and ecological concerns. Here, the microbiological quality of the oyster Crassostrea rhizophorae cultivated in a monitored environment and from natural beds in Bahia, northeastern Brazil, was determined. Bacterial diversity in oysters was measured by polymerase chain reaction-denaturing gradient gel electrophoresis. Sequence analysis revealed that most bacterial species showed similarity with uncultured or unidentified bacteria from environmental samples, and were clustered into the phylum Proteobacteria. Diverse bacteria from cultivated (monitored) oyster samples were grouped in the same cluster with a high similarity (2015) index (above 79%). Microbiological analyses revealed that these oysters did not contain pathogens. These results reflect the natural balance of the microbial communities essential to the maintenance of health and in inhibiting pathogen colonization in the oyster. On the other hand, bacterial diversity of samples from native stocks in extractive areas displayed a similarity index varying between 55 and 77%, and all samples were clustered separately from each other and from the cluster of samples derived from the cultivation area. Microbiological analyses showed that oysters from the extractive area were not fit for human consumption. This reflected a different composition of the microbial community in this area, probably resulting from anthropic impact. Our study also demonstrated that low temperatures and high rainfall limits the bacterial concentration in tropical oysters. This is the first study analyzing the total bacterial community profiles of the oyster C. rhizophorae.

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Molecular Analysis of Bacterial Microbiota Associated with Oysters (Crassostrea gigas and Crassostrea corteziensis) in Different Growth Phases at Two Cultivation Sites

Cited by 91 publications

References 65 publications

Hemolymph microbiome of Pacific oysters in response to temperature, temperature stress and infection

Hemolymph microbiome of Pacific oysters in response to temperature, temperature stress and infection

Technical note: use of internal transcribed spacer for ruminal yeast identification in dairy cows

Microbiological quality and bacterial diversity of the tropical oyster Crassostrea rhizophorae in a monitored farming system and from natural stocks

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