Technical note: use of internal transcribed spacer for ruminal yeast identification in dairy cows

Vargas‐Bello‐Pérez, Einar; Cancino‐Padilla, Nathaly; Romero, Jaime

doi:10.1017/s1751731116000768

Cited by 3 publications

(4 citation statements)

References 34 publications

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“…Pichia kudriavzevii was isolated from all herds evaluated and corresponded to the largest number of isolates. This species has been isolated by other authors in lactating cows, dry cows and heifers (Lund ; Almeida et al ; Sirisan et al ; Vargas‐Bello‐Pérez et al ) and represents 84·21% of yeasts isolated from the rumen fluid of animals evaluated by Almeida et al (). Pichia kudriavzevii was reported as being the predominant species in corn and sugarcane silages (Santos et al ; Carvalho et al ).…”

Section: Discussionmentioning

confidence: 65%

“…Yeast populations of 3·54 and 6·69 log 10 CFU per ml of rumen fluid were found in animals raised in tropical conditions and fed with or without supplementation respectively (Almeida et al ). Previous studies indicate that yeasts are generally introduced into the rumen along with ingested feeds (Vargas‐Bello‐Pérez et al ) and the composition of the ruminal microbiota varies with diet (Almeida et al ; Henderson et al ) and geographical region (Sundset et al ).…”

Section: Discussionmentioning

confidence: 99%

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Identification and characterization of yeasts from bovine rumen for potential use as probiotics

et al. 2019

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Aims:The aim was to isolate, identify and characterize yeasts present in rumen fluid and to select strains showing potential as probiotics. Methods and Results: Rumen fluid was sampled from 4 herds of dairy and beef cattle and 77 yeast isolates were identified. Initial screening was based on the capacity to maintain viability in a medium with different ruminal conditions. A second screening in fresh rumen fluid to assess the growth of inoculated yeasts and evaluate in vitro neutral detergent fibre digestibility (NDF-D), pH and acid accumulation was conducted. The yeast population ranged from 3Á84 to 6Á76 log 10 CFU per ml. The main species of yeast found were Pichia kudriavzevii, Candida rugosa, C. pararugosa, C. ethanolica and Magnusiomyces capitatus. Strains CCMA 933 (C. rugosa) and CCMA 970 (C. pararugosa) showed greater ability to survive in ruminal fluid and stimulated the production of acids. Isolate CCMA 967 (C. ethanolica) survived and improved the NDF-D. Conclusion: Pichia kudriavzevii was the dominant yeast found in the cattle herds. Strains CCMA 933, CCMA 970 and CCMA 967 showed properties that could be useful as potential probiotics for cattle. Significance and Impact of the Study: This study was the first to select yeasts from the rumen fluid, with the potential to be used as probiotic, based on the ruminal conditions. the contact of the cell wall with the intestinal lumen triggers an immune response (Dhama et al. 2015).Yeasts also appear to stimulate the growth and activity of fibrolytic ruminal bacteria by providing vitamins and growth factors (Moya et al. 2017). However, contradictory results are reported regarding interactions of diets, animals and environment (Chung et al. 2011). There are reports in the literature that the presence of some species Journal of Applied Microbiology 127, 845--855

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Section: Discussionmentioning

confidence: 65%

Section: Discussionmentioning

confidence: 99%

Identification and characterization of yeasts from bovine rumen for potential use as probiotics

et al. 2019

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“…Even though some yeast species may survive in the rumen and enter there with animal food, they are frequently regarded as transient, nonfunctional microorganisms in this ecosystem. The primary rumen environment conditions include pH values between 5.3 and 7.1, a temperature between 38°C and 40°C, a concentration of volatile fatty acids between 39 and 190 mmol l −1 , and a humidity range between 82% and 90% ( Vargas-Bello-Perez et al ., 2016 ; Fernandes et al ., 2019 ). By modifying the ruminal environment to make it more hospitable for microorganisms, yeast can operate as a growth booster.…”

Section: Introductionmentioning

confidence: 99%

Effect of laser radiation on the growth of Rhodotorula mucilaginosa isolated from rumen fluid of cows in the Nineveh, Iraq

Abedi,

Kasha,

Zaker

et al. 2023

Open Vet J

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Background: The fungi Rhodotorula species are widespread airborne contaminants and are thought to be natural occupants of human skin, lungs, urine, and feces. Rhodotorula mucilaginosa, R. minuta, and R. glutinis are the three most prevalent species. Aim: The purpose of this study is to isolate Rhodotorula mucilaginosa from cow rumen fluid in the province of Mosul, as well as to determine how laser light irradiation affects the growth and morphological traits of this organism. Methods: From the fluid rumen of AL-restaki and AL-karadi cows, the Rhodotorula mucilaginosa was isolated. Using a traditional approach and the ID-Yst card system Vitek 2, isolated colonies were purified, recognized, and described. A semiconductor laser system with a power of 50 mW and a wavelength of 450 nm was used in the experiment to evaluate the light laser irradiation effects on the direct culture growth of Rhodotorula mucilaginosa under two light irradiation conditions of 30 and 60 minutes. Results: According to traditional methods and the ID-Yst card system Vitek 2, Rhodotorula mucilaginosa predominated 7/30 (23.3%), and these strains effectively thrived on medium containing dextrose agar as evidenced by the carotenoid pigments that gave their colonies a salmon-pink to coral-red hue. When compared to a control group where no laser was used, the impact of light laser irradiation was assessed 24 hours after the irradiation using a biomass (dry weight measuring yeast cell content in suspension) and microscopic analysis using Gram stain. The irregular shape of the cells and their link to one another were shown by macroscopic and microscopic examinations. The irradiated culture subculture on sabouraued dextrose agar and incubation at 37 C for 3 days demonstrated inhibited growth in 4/7 (57.1%) isolates. Also, there was no discernible difference between the control group and the Rhodotorula mucilaginosa biomass concentration under light irradiation circumstances (30 and 60 min). Conclusion: This was the first study identifying Rhodotorula mucilaginosa in Iraqi cow rumen fluid.

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“…Internal Transcribed Spacer (ITS) is a technique to obtain information on unknown fungi species by sequencing ITS DNA and then comparing the sequenced ITS sequences with known fungal ITS sequences. It is a method for analyzing the diversity of fungi (Vargas-Bello-Pérez et al, 2016). This study used ITS to investigate the effects of MOS on rumen microbial diversity in dairy cows focusing on the perspective of the diversity of rumen fungi.…”

Section: Introductionmentioning

confidence: 99%

Study on Role of Kisspeptin in Reproductive Development and Inter-Relationship With Endocrine Markers for Nili-Ravi Buffalo Bulls (Bubalus Bubalis)

2021

THE JAPS

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To study the effects of mannan oligosaccharides (MOS) on rumen fungal flora of dairy cows, four lactating Chinese Holstein dairy cows were randomly divided into two groups in the two-stage 2 × 2 cross-over design. The experimental group (MT) was fed with a basal ration and MOS were perfused orally 60 g/(d head). The control group (CK) was fed with a basal ration. The rumen fluid was collected through the oral cavity of dairy cows using a negative pressure suction device. 50ml rumen fluid of every cow was collected each time respectively before feeding (0 h) and 2h, 4h and 6h after feeding in a day. The rumen fungi were tested by ITS sequencing technology. The results showed that four phyla were identified in CK group and five in MT group. The dominant phyla in both groups were Ascomycota, Basidiomycota, Neocallimastigomycota, whose abundances were more than 90% of the total rumen fungi. At the level of genus, 74 genera were identified in CK group, while 91 genera in MT group. Several cellulolytic fungi genera showed a decreasing trend or decreased significantly, such as Neocallimastigaceae-NA. The previous experiments that shows the addition of mannan oligosaccharides promoted the proliferation of rumen bacteria, while the growth of rumen fungi was inhibited in this result, which may be due to the antagonism between rumen bacteria and fungi. The growth of cellulose-degrading fungi was inhibited to some extend after the addition of MOS in the diet. Candida and Zopfiella, closely related to mycotic mastitis and immunosuppression respectively, had decreasing trends in rumen with supplemental MOS in the diets of the dairy cows, indicating that MOS might play a positive role in inhibiting harmful fungi. The experimental result shows that mannan oligosaccharides in the diet of dairy cows inhibited the growth of cellulolytic fungi and the proliferation of harmful fungi. In addition, the results showed that there might be the antagonistic effect between rumen fungi and bacteria potentially, which should be focused on for further study.

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Technical note: use of internal transcribed spacer for ruminal yeast identification in dairy cows

Cited by 3 publications

References 34 publications

Identification and characterization of yeasts from bovine rumen for potential use as probiotics

Identification and characterization of yeasts from bovine rumen for potential use as probiotics

Effect of laser radiation on the growth of Rhodotorula mucilaginosa isolated from rumen fluid of cows in the Nineveh, Iraq

Study on Role of Kisspeptin in Reproductive Development and Inter-Relationship With Endocrine Markers for Nili-Ravi Buffalo Bulls (Bubalus Bubalis)

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