Photosensitive protein patterning with electrophoretic deposition

Takamatsu, Seiichi; Hoshino, Kazunori; Matsumoto, Kiyoshi; Miyasaka, Tsutomu; Shimoyama, Isao

doi:10.1587/elex.7.779

Cited by 3 publications

(2 citation statements)

References 7 publications

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Section: Other Active Biomoleculesmentioning

confidence: 99%

“…After deposition, the multilayers can be removed from the array and can be used for assembling with other nanomaterial blocks or layers, for example, the structures can be used as compact functional layers for biosensor applications. Takamatsu et al 87 patterned the surface of ITO glass slides with a protein by EPD to get a photosensor array. They used the photosensitive protein of bacteriorhodopsin (bR) for future development of a bioelectronics based image sensor.…”

Section: Biomacromoleculesmentioning

confidence: 99%

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Electrophoretic Deposition of Biological Macromolecules, Drugs, And Cells

Seuss

Boccaccını

2013

Biomacromolecules

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The use of biological entities in biotechnology and the biomedical field is of great interest as the biocompatibility and the functionality of naturally occurring is usually higher compared to other biomaterials, for example, synthetic polymers. Processing of natural biomolecules, including proteins like collagen and also living cells and bacteria, to develop medical devices, bioactive coatings, functionalized implants, tissue scaffolds, or biosensors, is however challenging. Electrophoretic deposition, a technique that takes advantage of the presence of charged particles or molecules in suitable solvents, is a low-temperature process suitable for manipulating a wide range of biomolecules and biological entities preserving their bioactivity, which could be otherwise lost by processing at high temperatures. Another advantage of EPD is the possibility to use aqueous suspensions to process biological entities given that organic solvents also could lead to degradation of biomolecules. This paper gives an overview of the available literature on the application of EPD to process different biomolecules and biological entities, like proteins, bacteria cells, hyaluronic acid, and therapeutic drugs, aiming at using such biomaterials in numerous applications ranging from biosensors to orthopedic implants, tissue scaffolds, and drug delivery devices.

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Section: Other Active Biomoleculesmentioning

confidence: 99%

Section: Biomacromoleculesmentioning

confidence: 99%

Electrophoretic Deposition of Biological Macromolecules, Drugs, And Cells

Seuss

Boccaccını

2013

Biomacromolecules

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Fabrication of a Retinal‐protein Photoreceptor Array

et al. 2012

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A pixel‐architecture film of retinal proteins was prepared by an approach combining chemical, physical and biological technologies. Oriented multilayers of purple membrane composed of bacteriorhodopsin (BR) and lipids were patterned on an array of gold electrode pixels. In order to improve stability and resolution, the gene engineering technique was employed to make a mutant of the protein BR by replacing the 36th amino acid residue from aspartic acid to cysteine with a thiol end group ready to react with gold; electric sedimentation was used to guarantee the high probability of formation of the Au‐S bond and meanwhile to orient BR; further chemical crosslinking was introduced among layers of purple membranes to significantly enhance photoelectrical signals while keeping high stability. The non‐bound BR region was eventually washed out by detergent, and the remaining BR pixels were thus detergent resistant due to chemical crosslinking among BR layers and covalent binding between the multilayer and the substrate. The protein array was confirmed to keep photoelectrical activity.

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The photo charge of a bacterioRhodopsin electrochemical cells measured by a charge amplifier

Takamatsu

Hoshino

Matsumoto

et al. 2011

IEICE Electron. Express

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Abstract:We propose that electrochemical cells based on bacterioRhodopsin (bR), a photosensitive protein, generate electric charges under illuminated light and that a suitable signal detection apparatus for this cell type is a charge amplifier. By using charge amplifiers, we observed bR-photocell electric charges that were proportional to the intensity of incident light, which ranged from 0 to 8 mW/cm 2 . Then, the 4 × 4 micro-patterned bR photosensor array was connected to charge amplifiers to form an image sensor system. Static and moving images were detected with the constructed image sensor system. Keywords: photosensitive protein, bacterioRhodopsin, charge amplifier Classification: Micro-or nano-electromechanical systems

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Photosensitive protein patterning with electrophoretic deposition

Cited by 3 publications

References 7 publications

Electrophoretic Deposition of Biological Macromolecules, Drugs, And Cells

Electrophoretic Deposition of Biological Macromolecules, Drugs, And Cells

Fabrication of a Retinal‐protein Photoreceptor Array

The photo charge of a bacterioRhodopsin electrochemical cells measured by a charge amplifier

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