Photoprotection by Punica granatum seed oil nanoemulsion entrapping polyphenol-rich ethyl acetate fraction against UVB-induced DNA damage in human keratinocyte (HaCaT) cell line

Baccarin, Thaisa; Mitjans, Montserrat; Ramos, David; Lemos‐Senna, Elenara; Vinardell, M. Pilar

doi:10.1016/j.jphotobiol.2015.09.005

Cited by 64 publications

(31 citation statements)

References 47 publications

(50 reference statements)

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“…There are few reports showing PSO nanoemulsions preparation, employing different methods as sonication (Mizrahi et al 2014), self-nanoemulsifying (Lu et al 2015), ultrasonic emulsification/solvent evaporation (Baccarin & Lemos-Senna 2014;Baccarin et al 2015aBaccarin et al , 2015b. Recently, our group developed PSO nanoemulsion by nanoemulsification, employing pullulan as stabilizer (Ferreira et al 2015).…”

Section: Discussionmentioning

confidence: 99%

Pomegranate seed oil nanoemulsions with selective antiglioma activity: optimization and evaluation of cytotoxicity, genotoxicity and oxidative effects on mononuclear cells

Ferreira

Gehrcke

Cervi

et al. 2016

Pharmaceutical Biology

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(2016) Pomegranate seed oil nanoemulsions with selective antiglioma activity: optimization and evaluation of cytotoxicity, genotoxicity and oxidative effects on mononuclear cells, Pharmaceutical Biology, 54:12, 2968-2977, DOI: 10.1080/13880209.2016 Context: Glioma is a malignant brain tumor with rapid proliferation, infiltrative growth, poor prognosis and it is chemoresistent. Pomegranate seed oil (PSO) has antioxidant, anti-inflammatory and antitumor properties. This study showed the optimization of PSO nanoemulsions (NEs) as an alternative for glioma treatment.Objective: The study aimed to evaluate PSO NEs cytotoxicity on human blood cells and antiglioma effects against C6 cells. Materials and methods: NEs were prepared by the spontaneous emulsification method, using PSO at 1.5 and 3.0%, and were evaluated regarding their physical stability and antioxidant activity. Toxicity evaluations in human blood cells were performed in terms of cell viability, genotoxicity, lipid peroxidation, protein carbonylation, catalase activity and hemolysis at 0.1, 0.25 and 0.5 mg/mL PSO, after a 72-h incubation period. In vitro antitumor effect was determined against glioma cells after 24 and 48 h, and astrocytes were used as a non-transformed cell model. Results: Formulations presented droplet size below 250 nm, low polydispersity index, negative zeta potential and pH in the acid range. NEs and PSO had scavenging capacity around 30% and promoted a proliferative effect in mononuclear cells, increasing about 50% cell viability. No genotoxic and oxidative damage was observed in lipid peroxidation, protein carbonylation and catalase activity evaluations for NEs. Hemolysis study showed a hemolytic effect at high concentrations. Moreover, formulations reduced only tumor cell viability to 47%, approximately. Discussion and conclusion: Formulations are adequate and safe for intravenous administration. Besides, in vitro antitumor activity indicates that NEs are promising for glioma treatment.ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 99%

Pomegranate seed oil nanoemulsions with selective antiglioma activity: optimization and evaluation of cytotoxicity, genotoxicity and oxidative effects on mononuclear cells

Ferreira

Gehrcke

Cervi

et al. 2016

Pharmaceutical Biology

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“…Regarding pomegranate, the fruit has several medicinal properties and different parts of the fruit (peel, juice etc) can be used. Its main active compounds would be phenolic compounds [16]. Salvia species have also been used in wound treatment in folk medicine.…”

Section: Introductionmentioning

confidence: 99%

FTIR analysis and quantification of phenols and flavonoids of five commercially available plants extracts used in wound healing

et al. 2016

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Natural products are used in wound healing in order to prevent infection. Propolis is a well known antimicrobial with phenolic compounds and flavonoid content which vary according to the propolis origin. Besides propolis (from both Brazilian and UK sources), pomegranate, dragon's blood and sage are possible antimicrobials to be used in biomaterials. The goal of this work was to analyze the amount of phenols and flavonoid compounds in these natural products, their antioxidant activities and the bonds present by FTIR. The FTIR analysis revealed the presence of active compounds in all drug samples. The phenols quantification showed that Brazilian propolis was rich in phenols compared to the other drugs, followed by pomegranate and UK propolis. UK propolis was the most rich in flavonoids, which is expected on account of its origin. Pomegranate, UK propolis and Dragon's blood presented the highest antioxidant activity. All samples presented antioxidant activity > 82%.

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“…Increase in IL‐8 leads to angiogenesis, chemotaxis, tumorigenesis and cell proliferation . UVB‐induced increase in IL‐8 was inhibited by pretreatment of HaCaT cells with pomegranate seed oil nanoemulsions .…”

Section: Pomegranate Fruit Extractmentioning

confidence: 99%

Phytochemicals for the Prevention of Photocarcinogenesis

Pearlman

McClees

et al. 2017

Photochem & Photobiology

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Ultraviolet (UV) exposure has an array of damaging effects and is the main cause of skin cancer in humans. Non-melanoma skin cancer (NMSC), including basal cell carcinoma and squamous cell carcinoma, is the most common type of cancer. Incidence of NMSC has increased due to greater UV radiation, increased life expectancy, and other changes in lifestyle; the annual cost of skin cancer treatment in the United States has increased concurrently to around eight billion dollars. Because of these trends, novel approaches to skin cancer prevention have become an important area of research to decrease skin cancer morbidity and defray the costs associated with treatment. Chemoprevention aims to prevent or delay the development of skin cancer through the use of phytochemicals. Use of phytochemicals as chemopreventive agents has gained attention due to their low toxicity and anti-carcinogenic properties. Phytochemicals also exhibit antioxidant, anti-inflammatory, and anti-proliferative effects which support their use as chemopreventive agents, particularly for skin cancer. Preclinical and human studies have shown that phytochemicals decrease UV-induced skin damage and photocarcinogenesis. In this review article, we discuss the selected phytochemicals that may prevent or delay UV-induced carcinogenesis and highlights their potential use for skin protection.

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Photoprotection by Punica granatum seed oil nanoemulsion entrapping polyphenol-rich ethyl acetate fraction against UVB-induced DNA damage in human keratinocyte (HaCaT) cell line

Abstract: There has been an increase in use of botanicals as skin photoprotective agents.

Cited by 64 publications

References 47 publications

Pomegranate seed oil nanoemulsions with selective antiglioma activity: optimization and evaluation of cytotoxicity, genotoxicity and oxidative effects on mononuclear cells

Pomegranate seed oil nanoemulsions with selective antiglioma activity: optimization and evaluation of cytotoxicity, genotoxicity and oxidative effects on mononuclear cells

FTIR analysis and quantification of phenols and flavonoids of five commercially available plants extracts used in wound healing

Phytochemicals for the Prevention of Photocarcinogenesis

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