FTIR analysis and quantification of phenols and flavonoids of five commercially available plants extracts used in wound healing

Honey and propolis are natural substances produced by Apis mellifera that contain flavonoids, phenolic acids, and several other phytochemicals. The aim of this study was to phytochemically characterize three different types of honey and propolis, both separately and mixed, and to evaluate their wound-healing activity. Total phenolic compounds and flavonoids were determined using the Folin-Ciocalteu's and aluminum chloride colorimetric methods, respectively. The antioxidant activity was evaluated by both the DPPH free radical scavenging assay and β-carotene bleaching test, and the anti-inflammatory activity was determined by a protein denaturation method. To evaluate the wound-healing activity of the samples, NHDF cells were subjected to a wound scratch assay. The obtained results showed that dark-brown honey presents a higher concentration of phenolic compounds and flavonoids, as well as higher antioxidant and anti-inflammatory activities. Propolis samples had the highest concentrations in bioactive compounds. Examining the microscopic images, it was possible to verify that the samples promote cell migration, demonstrating the wound-healing potential of honey and propolis.

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“…The results now obtained are in agreement with other previously published results for other honey and propolis samples [20,21].…”

Section: Ftir Analysis Of the Samples Of Honey Propolis And Propolisupporting

confidence: 93%

Evaluation of the In Vitro Wound-Healing Activity and Phytochemical Characterization of Propolis and Honey

et al. 2020

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“…Polyvinyl alcohol—PVA ( M W = 85,000–124,000, 99+% hydrolized) and sodium carboxymethyl cellulose—NaCMC (average M W ∼250,000) were purchased from Sigma‐Aldrich. A Brazilian green propolis alcoholic extract without wax (minimum of 11% propolis), previously characterized by our group , was purchased from Uniflora ® .…”

Section: Methodsmentioning

confidence: 99%

Absorbent polyvinyl alcohol–sodium carboxymethyl cellulose hydrogels for propolis delivery in wound healing applications

Oliveira

Moreira

Thiré

et al. 2017

Polymer Engineering & Sci

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Second degree burns require dressings to heal. The ideal dressing should keep a moist environment, have low cost, be elastic and have a bactericidal effect. The potential of PVA-NaCMC (sodium carboxymethyl cellulose) gels to combine the mechanical and swelling properties of PVA with the flexibility and high water uptake of NaCMC and the antimicrobial characteristics of propolis is investigated. The freeze-thawed gels were characterized by FTIR and DSC. Their swelling behavior, their mechanical response, the delivery of active compounds and their antimicrobial properties were also determined. The main findings of the FTIR analysis were that no chemical bonding occurred between the materials. The DSC analysis revealed that the addition of NaCMC to PVA lowered the PVA crystallinity, as did the addition of propolis, leading to a more deformable gel (as can be observed from the tensile tests results). This also resulted in a higher rate of delivery of active compounds, higher weight loss and higher fluid uptake than comparable PVA-propolis systems, as shown by the swelling tests and by the propolis delivery tests. Samples with 15% propolis content or more inhibited S. aureus colonies with 80% reduction, and are therefore highly absorbent and compliant antimicrobial gels for wound healing.

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“…The characteristic bands and vibration modes from literature for alginate, neomycin, and propolis are given in Supporting Information. All samples' spectra show a wide band in 3250 cm −1 characteristic of the presence of ─OH groups in their structure . In addition, this band could also be relative to the presence of water in the samples that correspond to their manufacture .…”

Section: Resultsmentioning

confidence: 99%

“…All samples' spectra show a wide band in 3250 cm −1 characteristic of the presence of ─OH groups in their structure. 27,60,61 In addition, this band could also be relative to the presence of water in the samples that correspond to their manufacture. 62,63 After the second stage of cross-linking, the samples were dried at room temperature, which was not capable of removing all residual water present in the samples.…”

Section: Microstructural Analysismentioning

confidence: 99%

Alginate hydrogels incorporating neomycin or propolis as potential dressings for diabetic ulcers: Structure, swelling, and antimicrobial barrier properties

Candido

Conceição

Moreira

et al. 2019

Polymers for Advanced Techs

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Alginate hydrogels have many attractive characteristics for potential use as wound dressing materials. However, they are not considered to possess any intrinsic activity against microbial infection, often present in neuropathic wounds. To overcome this, the effect of incorporating neomycin or propolis in alginate hydrogels was investigated, both by direct blending alone and also by further addition of loaded alginate microparticles prepared by the extrusion dripping method. The morphological, microstructural, thermal, mechanical, and swelling properties of each film were evaluated, as well as particle size distribution and antimicrobial penetration analysis. Microparticle size was considered suitable for drug delivery applications and incorporation in hydrogel films. The presence of neomycin and propolis, in both blended and microparticle form, interfered with film properties leading to hydrogels with different characteristics. All samples showed swelling degrees up to 100% and mechanical and thermal properties suitable for application as wound dressings. In addition, all samples acted as barriers to microbial penetration.

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FTIR analysis and quantification of phenols and flavonoids of five commercially available plants extracts used in wound healing

Cited by 257 publications

References 41 publications

Evaluation of the In Vitro Wound-Healing Activity and Phytochemical Characterization of Propolis and Honey

Evaluation of the In Vitro Wound-Healing Activity and Phytochemical Characterization of Propolis and Honey

Absorbent polyvinyl alcohol–sodium carboxymethyl cellulose hydrogels for propolis delivery in wound healing applications

Alginate hydrogels incorporating neomycin or propolis as potential dressings for diabetic ulcers: Structure, swelling, and antimicrobial barrier properties

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