Photoelectrochemical determination for acid phosphatase activity based on an electron inhibition strategy

Huang, Mingjuan; Tian, Jianhua; Zhou, Chunhong; Bai, Ping; Lu, Jianzhong

doi:10.1016/j.snb.2020.127654

Cited by 18 publications

(6 citation statements)

References 59 publications

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“…Previous studies on the covalent grafting of diazonium salt on graphitic carbon nitride (g-C 3 N 4 ) have shown a decrease in photocurrent generation with respect to the bare electrode material. , In this study, all the covalently modified electrodes show a much higher photocurrent output compared to the control sample (bare FTO and FTO/SLG), confirming the great potential of the diazonium-derived wires, especially in the case of FTO/SLG electrodes (Figure ). From −100 mV to the anodic region, both metal-based wires show roughly similar photocurrent output.…”

Section: Resultssupporting

confidence: 78%

Diazonium-Based Covalent Molecular Wiring of Single-Layer Graphene Leads to Enhanced Unidirectional Photocurrent Generation through the p-doping Effect

et al. 2022

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Development of robust and cost-effective smart materials requires rational chemical nanoengineering to provide viable technological solutions for a wide range of applications. Recently, a powerful approach based on the electrografting of diazonium salts has attracted a great deal of attention due to its numerous technological advantages. Several studies on graphene-based materials reveal that the covalent attachment of aryl groups via the above approach could lead to additional beneficial properties of this versatile material. Here, we developed the covalently linked metalorganic wires on two transparent, cheap, and conductive materials: fluorine-doped tin oxide (FTO) and FTO/single-layer graphene (FTO/SLG). The wires are terminated with nitrilotriacetic acid metal complexes, which are universal molecular anchors to immobilize His6-tagged proteins, such as biophotocatalysts and other types of redox-active proteins of great interest in biotechnology, optoelectronics, and artificial photosynthesis. We show for the first time that the covalent grafting of a diazonium salt precursor on two different electron-rich surfaces leads to the formation of the molecular wires that promote p-doping of SLG concomitantly with a significantly enhanced unidirectional cathodic photocurrent up to 1 μA cm–2. Density functional theory modeling reveals that the exceptionally high photocurrent values are due to two distinct mechanisms of electron transfer originating from different orbitals/bands of the diazonium-derived wires depending on the nature of the chelating metal redox center. Importantly, the novel metalorganic interfaces reported here exhibit minimized back electron transfer, which is essential for the maximization of solar conversion efficiency.

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Section: Resultssupporting

confidence: 78%

Diazonium-Based Covalent Molecular Wiring of Single-Layer Graphene Leads to Enhanced Unidirectional Photocurrent Generation through the p-doping Effect

et al. 2022

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“…Compared to other detection methods (Table S1 in ESM), the method is competitive to detect ACP as low as 0.04 mU/mL (372 nm) and 0.38 mU/mL (614 nm) (signal/noise = 3) [38], and the linear ranges are 0.13-5.00 mU/mL and 1.25-20.00 mU/mL, respectively, lower than that reported with C-QDs [39] and with proton nuclear magnetic resonance [40]. Although the detection limit is higher than electrochemistry assay [41], the proposed method is more feasible without tedious preparation. Compared to the fluorescence assay based on N-CDs [42], the proposed method exhibits a shorter hydrolysis time.…”

Section: Application Of Acp Assaymentioning

confidence: 67%

A dual-signal fluorescent probe for detection of acid phosphatase

Wang

et al. 2021

Anal Bioanal Chem

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Acid phosphatase has become a significant indicator of prognostic and medical diagnosis, and its dysfunction may lead to a series of diseases. A novel dual-signal fluorescence method for acid phosphatase detection based on europium polymer (europiumpyridine dicarboxylicacid-adenine) and pyridoxal phosphate (PLP) was proposed. PLP coordinated with europium polymer via Eu 3+ and P-O bonds, and the fluorescence of europium polymer was quenched due to the photoinduced electron transfer (PET) effect between aldehyde and europium polymer. Upon addition of acid phosphatase, the PLP was transformed to phosphate (Pi) and pyridoxal (PL). The PL was released from the surface of europium polymer, and the blue emission was enhanced due to the formation of internal hemiacetal, while the fluorescence of europium polymer recovered. The blue (PL) and red emission (Eu 3+ ) were positively correlated with acid phosphatase activity; thus the sensitive assay of acid phosphatase was effectively achieved. The two signals were applied to determine the acid phosphatase with limits of detection (LOD) of 0.04 mU/mL and 0.38 mU/mL, and the linear ranges were 0.13-5.00 mU/mL and 1.25-20.00 mU/mL, respectively. The probe can be used to trace the acid phosphatase in biological systems and holds promise for use in clinical diagnosis and early prevention.

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“…Huang et al. developed a novel PEC sensor‐based platform for determining acid phosphatase (ACP), [ 117 ] which is a common hydrolase in human tissues and fluids and catalyzes the hydrolysis of phosphate monoesters, which plays a critical role in several important processes. [ 118 ] Abnormal expression of ACP in the body is a vital indicator of many diseases, such as prostate cancer, Gaucher's disease, hyperparathyroidism, multiple myeloma.…”

Section: Applications Of G‐c3n4 In Signal‐transducing Agent For Sensomentioning

confidence: 99%

“…Huang and co‐workers have developed a TNA/g‐C 3 N 4 /3,5‐diamino‐1,2,4‐triazole (DAT) sensor to utilize the electron inhibition performance of DAT and the coordination of Fe(II) with DAT (Figure 9A). [ 117 ] This work involved electrochemically grafting DAT diazonium salt onto the surface of the TNA/g‐C 3 N 4. The results were that the PEC signal response decreased significantly because of the electron hindrance effect of the DAT films.…”

Section: Applications Of G‐c3n4 In Signal‐transducing Agent For Sensomentioning

confidence: 99%

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Emerging tri‐s‐triazine‐based graphitic carbon nitride: A potential signal‐transducing nanostructured material for sensor applications

et al. 2020

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Today, tris‐s‐triazine based graphitic carbon nitride (g‐C3N4) is a new research hot topic. It has a unique electronic band structure, high physicochemical stability, large surface area, and is “earth‐abundant.” These and other properties have made it a highly researched material especially for visible light photocatalysis and photodegradation applications and as the starting material from which to develop novel electrochemical sensing platforms. In this review, the state‐of‐the‐art technologies utilizing tris‐s‐triazine graphitic carbon nitride as a tailorable signal‐transducing nanostructured material for sensing applications is presented in detail. Initially, the electronic structure of g‐C3N4, morphologies, doping, heterojunctions, its combination with other carbon materials, and defect formation, is described, which is followed by a discussion on its role in electrochemiluminescence, photoelectrochemical, fluorescence sensors and gas sensors as a signal transducer with appropriate examples. This review concludes with a discussion summarizing state‐of‐the‐art and both future perspectives and challenges at the cutting edge of this research.

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Photoelectrochemical determination for acid phosphatase activity based on an electron inhibition strategy

Cited by 18 publications

References 59 publications

Diazonium-Based Covalent Molecular Wiring of Single-Layer Graphene Leads to Enhanced Unidirectional Photocurrent Generation through the p-doping Effect

Diazonium-Based Covalent Molecular Wiring of Single-Layer Graphene Leads to Enhanced Unidirectional Photocurrent Generation through the p-doping Effect

A dual-signal fluorescent probe for detection of acid phosphatase

Emerging tri‐s‐triazine‐based graphitic carbon nitride: A potential signal‐transducing nanostructured material for sensor applications

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