A dual-signal fluorescent probe for detection of acid phosphatase

Abstract

Acid phosphatase has become a significant indicator of prognostic and medical diagnosis, and its dysfunction may lead to a series of diseases. A novel dual-signal fluorescence method for acid phosphatase detection based on europium polymer (europiumpyridine dicarboxylicacid-adenine) and pyridoxal phosphate (PLP) was proposed. PLP coordinated with europium polymer via Eu 3+ and P-O bonds, and the fluorescence of europium polymer was quenched due to the photoinduced electron transfer (PET) effect between aldehyde and europium polymer. Upon addition of acid phosphatase, the PLP was transformed to phosphate (Pi) and pyridoxal (PL). The PL was released from the surface of europium polymer, and the blue emission was enhanced due to the formation of internal hemiacetal, while the fluorescence of europium polymer recovered. The blue (PL) and red emission (Eu 3+ ) were positively correlated with acid phosphatase activity; thus the sensitive assay of acid phosphatase was effectively achieved. The two signals were applied to determine the acid phosphatase with limits of detection (LOD) of 0.04 mU/mL and 0.38 mU/mL, and the linear ranges were 0.13-5.00 mU/mL and 1.25-20.00 mU/mL, respectively. The probe can be used to trace the acid phosphatase in biological systems and holds promise for use in clinical diagnosis and early prevention.

Colorimetric and Fluorescent Dual-Modality Sensing Platform Based on Fluorescent Nanozyme

“…For the detection using a fluorescence signal channel, a linear detection range of 10∼5 mU/ ml with an LOD of 4.6 μU/ml (S/N 3) is obtained. The LOD is lower than that obtained using N-CDs, N-CDs-MnO 2 nanocomposites, or Eu 3+ -coordination polymer (Supplementary Table S1 in SI) (Zhu et al, 2018;Zhu et al, 2019;Li et al, 2021). To investigate the specificity of ACP detection, the detection system was, respectively, treated with ACP, bovine serum albumin, trypsin, glucose oxidase, pepsin, or lysozyme.…”