Photodynamic therapy using methylene blue in lung adenocarcinoma xenograft and hamster cheek pouch induced squamous cell carcinoma

Obstoy, Bérengère; Bohn, Pierre; Veresezan, Liana; Sesboüé, Richard; Thiberville, Luc

doi:10.1016/j.pdpdt.2016.03.003

Cited by 15 publications

(15 citation statements)

References 18 publications

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“…MB loaded H1 and H2 were degraded for 6 h in PBS and then both dark toxicity, impact of laser irradiation on the viability of untreated cells and the influence of PDT on cell viability were tested in a dose dependent manner using A549 cell lines. For this part, a lung cancer cell line was preferred because the lung is an appropriate target organ for phototherapies which can be performed via endoscopic methods [44,63]. Free MB ( 16µM) was also applied to cells at the highest concentration of MB in H1 and H2 degradation products.…”

Section: In Vitro Cytotoxicity and Photodynamic Therapymentioning

confidence: 99%

One-Step Injectable and Bioreducible Poly(β-Amino Ester) Hydrogels as Controlled Drug Delivery Platforms

Bingol

Altuncu

Duman

et al. 2019

ACS Appl. Polym. Mater.

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A one-step synthesis strategy based on aza-Michael reaction of poly(ethylene glycol) diacrylate (PEGDA) or PEGDA/1,6-hexanediol diacrylate (HDDA) mixture and cystamine was employed to fabricate injectable, biocompatible and degradable novel poly(-amino ester) (PBAE) hydrogels.The gelation was monitored by real-time dynamic rheological measurements in order to follow formation of the PBAE hydrogel networks. The obtained hydrogels were responsive to both pH and redox state which enabled the control of swelling, degradation and release properties by external triggers. Degradation products of the hydrogels were shown to have no significant cytotoxicity on A549 adenocarcinomic human alveolar basal epithelial cells and MCF-7 human breast cancer cells. The hydrogels were loaded with a photosensitizer, methylene blue (MB), as a model compound by simply adding the MB molecules into the precursor mixture. The activity of released MB was assessed by in vitro photodynamic therapy (PDT) studies conducted with A549 cells.

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Section: In Vitro Cytotoxicity and Photodynamic Therapymentioning

confidence: 99%

One-Step Injectable and Bioreducible Poly(β-Amino Ester) Hydrogels as Controlled Drug Delivery Platforms

Bingol

Altuncu

Duman

et al. 2019

ACS Appl. Polym. Mater.

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“…MB, as we also observed, is relative unselective, for better targeting several research groups try to pack it into nanoparticles as published by Usacheva and coauthors [ 48 ]. MB as a PDT agent for the treatment of respiratory tract cancer in animal models was described to be efficient [ 71 ]. Further studies are needed to assess the safety and efficacy of MB-associated PDT for the treatment of cancer in humans.…”

Section: Discussionmentioning

confidence: 99%

Photodynamic Effect of Methylene Blue and Low Level Laser Radiation in Head and Neck Squamous Cell Carcinoma Cell Lines

Kofler

Romani

Pritz

et al. 2018

IJMS

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Photodynamic therapy (PDT) is suggested to have an impact on the treatment of early stage head and neck cancers (HNSCC). We investigated the effect of PDT with methylene blue (MB) and a diode laser (660 nm) as the laser source on HNSCC cell lines as an in vitro model of surface oral squamous cell carcinoma. Cell-cultures were exposed to 160 µM MB for 4 min and to laser light for 8 min. Viability was proven via cell viability assay and clonogenic survival via clone counting assay. The combination of MB and diode laser evidenced high efficient loss of cell viability by 5% of the control, while treatment with the same concentration of MB for 4 min alone showed a viability of 46% of the control. In both SCC-25 and Detroit 562 HNSCC cells, MB combined with the laser allowed a significant abrogation of clonogenic growth (p < 0.01), especially in the case of Detroit 562 cells less than 1% of the suspension plated cells were able to grow tumor cell nests. Multiresistant (Detroit 562) HNSCC cells expressing cancer stem cell markers are sensitive to MB/red laser combined PDT.

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“…Upon light exposure, the photosensitizer (PS) can undergo 1 of 2 types of reaction, type I or type II reaction, 53 resulting in the generation of free radicals and reactive oxygen species, which cause oxidative stress and cell damage. There are many PS, such as phthalocyanine, 54 hypericin, 55 methylene blue (MB), 56 and pheophorbide‐a, 57 that can be used in PDT. However, most PS have low solubility, poor cell/tissue penetration, and lack of selectivity, leading to low PDT efficacy.…”

Section: Applications As Delivery Vehicles In Cancer Therapymentioning

confidence: 99%

Tetrahedral DNA nanostructures as drug delivery and bioimaging platforms in cancer therapy

2020

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Structural DNA nanotechnology enables DNA to be used as nanomaterials for novel nanostructure construction with unprecedented functionalities. Artificial DNA nanostructures can be designed and generated with precisely controlled features, resulting in its utility in bionanotechnological and biomedical applications. A tetrahedral DNA nanostructure (TDN), the most popular DNA nanostructure, with high stability and simple synthesis procedure, is a promising candidate as nanocarriers in drug delivery and bioimaging platforms, particularly in precision medicine as well as diagnosis for cancer therapy. Recent evidence collectively indicated that TDN successfully enhanced cancer therapeutic efficiency both in vitro and in vivo. Here, we summarize the development of TDN and highlight various aspects of TDN applications in cancer therapy based on previous reports, including anticancer drug loading, photodynamic therapy, therapeutic oligonucleotides, bioimaging platforms, and other molecules and discuss a perspective in opportunities and challenges for future TDN‐based nanomedicine.

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Photodynamic therapy using methylene blue in lung adenocarcinoma xenograft and hamster cheek pouch induced squamous cell carcinoma

Abstract: Intratumoral MB appears efficient as PDT agent for cancer treatment in animal models. Further studies are needed to assess the safety and efficacy of MB-associated PDT for the treatment of lung cancer in humans.

Cited by 15 publications

References 18 publications

One-Step Injectable and Bioreducible Poly(β-Amino Ester) Hydrogels as Controlled Drug Delivery Platforms

One-Step Injectable and Bioreducible Poly(β-Amino Ester) Hydrogels as Controlled Drug Delivery Platforms

Photodynamic Effect of Methylene Blue and Low Level Laser Radiation in Head and Neck Squamous Cell Carcinoma Cell Lines

Tetrahedral DNA nanostructures as drug delivery and bioimaging platforms in cancer therapy

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