Photodynamic Effect of Methylene Blue and Low Level Laser Radiation in Head and Neck Squamous Cell Carcinoma Cell Lines

Kofler, Barbara; Romani, Angela; Pritz, Christian; Steinbichler, Teresa Bernadette; Schartinger, Volker Hans; Riechelmann, Herbert; Dudás, József

doi:10.3390/ijms19041107

Cited by 56 publications

(28 citation statements)

References 65 publications

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“…One-thousand cells from all samples were plated in 75-cm 2 cell culture flasks grown in serum-supplemented medium for 3 weeks and the growing colonies were stained with gentian violet and were counted. The number of growing colonies were related to plated cell numbers (1000) [57].…”

Section: Methodsmentioning

confidence: 99%

Brain-Derived Neurotrophin and TrkB in Head and Neck Squamous Cell Carcinoma

Dudás

Riml

Tuertscher

et al. 2019

IJMS

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We hypothesized that in head and neck squamous cell carcinoma (HNSCC), the neurotrophin brain-derived neurotrophic factor (BDNF) and its high affinity receptor TrkB regulate tumor cell survival, invasion, and therapy resistance. We used in situ hybridization for BDNF and immunohistochemistry (IHC) for TrkB in 131 HNSCC samples. Brain-derived neurotrophic factor was highly expressed in normal mucosa in HNSCC tissue and in cell lines, whereas only 42.74% of HNSCC tissue was TrkB+. One fourth of HNSCC cases was human papilloma virus (HPV)− positive, but the TrkB IHC frequency was not different in HPV-positive (HPV+) and negative cases. The UPCI-SCC090 cells expressed constitutive levels of TrkB. Transforming-growth-factor-β1 (1 ng/mL TGF-β1) induced TrkB in a subpopulation of SCC-25 cells. A single 10-µg/mL mitomycin C treatment in UPCI-SCC090 cells induced apoptosis and BDNF did not rescue them. The SCC-25 cells were resistant to the MMC treatment, and their growth decreased after TGF-β1 treatment, but was restored by BDNF if it followed TGF-β1. Taken together, BDNF might be ineffective in HPV+ HNSCC patients. In HPV− HNSCC patients, tumor cells did not die after chemotherapeutic challenge and BDNF with TGF-β1 could improve tumor cell survival and contribute to worse patient prognosis.

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Section: Methodsmentioning

confidence: 99%

Brain-Derived Neurotrophin and TrkB in Head and Neck Squamous Cell Carcinoma

Dudás

Riml

Tuertscher

et al. 2019

IJMS

Self Cite

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“…Mouse Gardner lymphoma, sarcoma 180, Ehrlich ascites, and mammary adenocarcinoma have all been successfully treated using MB in animal models [82]. Likewise, some human cancer cells have been reported as responders of MB-mediated light therapy, including HeLa cervical adenocarcinoma cells, early stage head and neck squamous cell carcinoma (HNSCC), and lung adenocarcinoma cells [83][84][85]. Although some studies have indicated that the cytotoxicity of MB can be improved by chemical modifications, these studies are beyond the scope of this review, particularly considering the functionality of the safe, proven molecules in the treatment of a variety of cancers using MB-mediated light therapy.…”

Section: In-vitro Observation Of Methylene Blue (Mb)-mediated Pdt In Cancer Treatmentmentioning

confidence: 99%

Methylene Blue-Based Nano and Microparticles: Fabrication and Applications in Photodynamic Therapy

Lim

2021

Polymers

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Methylene blue (MB) has been used in the textile industry since it was first extracted by the German chemist Heinrich Caro. Its pharmacological properties have also been applied toward the treatment of certain diseases such as methemoglobinemia, ifosfamide-induced encephalopathy, and thyroid conditions requiring surgery. Recently, the utilization of MB as a safe photosensitizer in photodynamic therapy (PDT) has received attention. Recent findings demonstrate that photoactivated MB exhibits not only anticancer activity but also antibacterial activity both in vitro and in vivo. However, due to the hydrophilic nature of MB, it is difficult to create MB-embedded nano- or microparticles capable of increasing the clinical efficacy of the PDT. This review aims to summarize fabrication techniques for MB-embedded nano and microparticles and to provide both in vitro and in vivo examples of MB-mediated PDT, thereby offering a future perspective on improving this promising clinical treatment modality. We also address examples of MB-mediated PDT in both cancer and infection treatments. Both in-vitro and in-vivo studies are summarized here to document recent trends in utilizing MB as an effective photosensitizer in PDT. Lastly, we discuss how developing efficient MB-carrying nano- and microparticle platforms would be able to increase the benefits of PDT.

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“…To quantify the relative photoactivity of MPPa and NMPi in the absence of tumor cells, 1 O 2 photogeneration was measured by 1,3-diphenylisobenzofuran (DPBF) as a selective 1 O 2 acceptor ( Figure 5 b). NMPi presented slightly better 1 O 2 photogeneration than MPPa, and NMPi was almost comparable to methylene blue (MB) as a standard 1 O 2 sensitizer [ 39 ]. This result was consistent with the cell viability results shown in Figure 2 and Figure 3 .…”

Section: Resultsmentioning

confidence: 99%

Tumor Size-Dependent Anticancer Efficacy of Chlorin Derivatives for Photodynamic Therapy

Chang

Liu

Lee

et al. 2018

IJMS

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Photodynamic therapy (PDT) with a suitable photosensitizer molecule is a promising anticancer treatment. We evaluated two chlorin molecules as potential photosensitizers, methyl pyropheophorbide a (MPPa) and N-methoxyl purpurinimide (NMPi), against A549 human lung adenocarcinoma cells in vitro as well as in A549 tumor-bearing mice in vivo. Cell viability, microscopy, and fluorescence-activated cell sorting (FACS) analyses were performed for the in vitro studies. MPPa and NMPi showed high phototoxicity in vitro, which was dependent on the concentration of the photosensitizers as well as the light irradiation time. In the animal study, tumor volume change, tumor surface alterations, and hematoxylin & eosin (H&E) and terminal deoxyribonucleotidyl transferse-mediated dUTP nick-end labelling (TUNEL) staining analyses were performed and compared between small (tumor volume of <50 mm3) and large (tumor volume of >50 mm3) size of initial tumors. MPPa and NMPi showed high anticancer efficacy against small-size tumors, indicating that early treatment with PDT is effective. Especially, repeated two times PDT with NMPi allowed almost complete eradication against small-size tumors. However, MPPa and NMPi were not effective against large-size tumors. In conclusion, the two chlorin derivatives, MPPa and NMPi, show good anticancer efficacy as promising photosensitizers for PDT in vitro and in vivo. Moreover, their activity in vivo was significantly dependent on the initial tumor size in mice, which confirms the importance of early cancer treatment.

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Photodynamic Effect of Methylene Blue and Low Level Laser Radiation in Head and Neck Squamous Cell Carcinoma Cell Lines

Cited by 56 publications

References 65 publications

Brain-Derived Neurotrophin and TrkB in Head and Neck Squamous Cell Carcinoma

Brain-Derived Neurotrophin and TrkB in Head and Neck Squamous Cell Carcinoma

Methylene Blue-Based Nano and Microparticles: Fabrication and Applications in Photodynamic Therapy

Tumor Size-Dependent Anticancer Efficacy of Chlorin Derivatives for Photodynamic Therapy

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