Photodynamic inactivation of planktonic cultures and Streptococcus mutans biofilms for prevention of white spot lesions during orthodontic treatment: An in vitro investigation

Esper, Maria Ângela Lacerda Rangel; Junqueira, Juliana Campos; Fernandes, Adjaci Uchôa; Bresciani, Eduardo; Rastelli, Alessandra Nara de Souza; Navarro, Ricardo Scarparo; Gonçalves, Sérgio Eduardo de Paiva

doi:10.1016/j.ajodo.2018.03.027

Cited by 20 publications

(15 citation statements)

References 50 publications

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“…The systematic review showed that among the cariogenic microorganisms listed in the selected studies, the most studied microorganism was Streptococcus mutans (82%) [12,14,15,16,17,18,19,20,21,23,24,25,26,27,28,29,31,32,33,34,35,37,39,40,41,42,43,45].…”

Section: Resultsmentioning

confidence: 99%

“…The success of PDT depends on factors such as the administrated dose of light in the target cells and the time of exposure to light [46]. Considering these factors, we found that the most widely used light source was the red LED (32%) with wavelengths ranging from 625 to 670 nm [12,15,16,17,18,21,24,29,30,32,36,40], the most commonly used PS was methylene blue [13,16,18,21,23,24,27,28,29,30,32,33,36,38,39,42], and the most widely used pre-irradiation time was 5 min [12,14,15,16,17,19,20,21,22,27,28,30,31,32,33,39,42,43,45].…”

Section: Resultsmentioning

confidence: 99%

“…For a substance to be considered as a bactericide, it is necessary for a total inhibition of microorganism growth or ≤99.9% decrease in the initial inoculum (3-log 10 reduction in colony forming units [cfu]/mL) in the subculture [68]. From the 34 articles analyzed, only 11 presented a reduction greater than or equal to 99.9% [14,16,18,23,24,29,34,39,41,43,45]. This fact proves that eliminating these microorganisms is a great challenge, especially when they are in the biofilm.…”

Section: Discussionmentioning

confidence: 99%

“…Among the vitro studies, the biofilm models were more frequent [12,13,15,18,19,20,21,24,27,28,31,32,33,34,35,37,38,39,40,43,44,45] than the suspensions, which confirm the fidelity of the data, since in biofilms, the microorganisms interact with each other and are more resistant to the antimicrobial agents when compared to the microorganisms in suspension [75]. Many studies have used monotypic biofilms of S. mutans , but this model is less representative of the oral environment and underestimates the complexity of the dental biofilm [76], so we emphasize the importance of studies with multispecies biofilms.…”

Section: Discussionmentioning

confidence: 99%

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Effect of Photodynamic Therapy on Microorganisms Responsible for Dental Caries: A Systematic Review and Meta-Analysis

Oliveira

Ferrisse

Marques

et al. 2019

IJMS

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The aim of this study was to perform a systematic review of the literature followed by a meta-analysis about the efficacy of photodynamic therapy (PDT) on the microorganisms responsible for dental caries. The research question and the keywords were constructed according to the PICO strategy. The article search was done in Embase, Lilacs, Scielo, Medline, Scopus, Cochrane Library, Web of Science, Science Direct, and Pubmed databases. Randomized clinical trials and in vitro studies were selected in the review. The study was conducted according the PRISMA guideline for systematic review. A total of 34 articles were included in the qualitative analysis and four articles were divided into two subgroups to perform the meta-analysis. Few studies have achieved an effective microbial reduction in microorganisms associated with the pathogenesis of dental caries. The results highlight that there is no consensus about the study protocols for PDT against cariogenic microorganisms, although the results showed the PDT could be a good alternative for the treatment of dental caries.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of Photodynamic Therapy on Microorganisms Responsible for Dental Caries: A Systematic Review and Meta-Analysis

Oliveira

Ferrisse

Marques

et al. 2019

IJMS

View full text Add to dashboard Cite

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“…It can be excited by the light between 550 nm and 700 nm to produce photodynamic e®ect. [8][9][10] It is cheap, easy to produce and widely used in the treatment of periodontal and periimplant in°ammation. However, the e®ect of plaque bio¯lm is still controversial.…”

Section: Introductionmentioning

confidence: 99%

Effect of antibacterial photodynamic therapy on Streptococcus mutans plaque biofilm in vitro

Liang

Zou

et al. 2020

J. Innov. Opt. Health Sci.

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The main objective of this study is to evaluate the antibacterial effect of antibacterial photodynamic therapy (aPDT) on Streptococcus mutans (S. mutans) biofilm model in vitro. The selection of photosensitizers is the key step for the efficacy of photodynamic therapy (PDT). However, no studies have been conducted in the oral field to compare the functional characteristics and application effects of PDT mediated by various photosensitizers. In this research, the antibacterial effect of Methylene blue (MB)/650[Formula: see text]nm laser and Hematoporphyrin monomethyl ether (HMME)/532[Formula: see text]nm laser on S. mutans biofilm was compared under different energy densities to provide experimental reference for the clinical application of the two PDT. The yield of lactic acid was analyzed by Colony forming unit (CFU) and spectrophotometry, and the complete biofilm activity was measured by Confocal Laser Scanning Microscopy (CLSM) to evaluate the bactericidal effect on each group. Based on the results of CFU, the bacterial colonies formed by 30.4[Formula: see text]J/cm2 532[Formula: see text]nm MB-aPDT group and 30.4[Formula: see text]J/cm2 532[Formula: see text]nm HMME-aPDT group were significantly less than those in other groups, and the bacterial colonies in HMME-aPDT group were less than those in HMME-aPDT group. Lactic acid production in all treatment groups except the photosensitizer group was statistically lower than that in the normal saline control group. The activity of bacterial plaque biofilm was significantly decreased in the two groups treated with 30.4[Formula: see text]J/cm2 aPDT. Therefore, aPDT suitable for energy measurement can kill S. mutans plaque biofilm, and MB-aPDT is better than HMME-aPDT.

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The effect of photodynamic therapy in controlling the oral biofilm: A comprehensive overview

Aghili,

Jahangirnia,

Alam

et al. 2023

J Basic Microbiol

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Several resistance mechanisms are involved in dental caries, including oral biofilms. An accumulation of bacteria on the surface of teeth is called plaque. Periodontitis and gingivitis are caused by dental plaque. In this review article, we aimed to review the studies associated with the application of photodynamic therapy (PDT) to prevent and treat various microbial biofilm‐caused oral diseases in recent decades. There are several studies published in PubMed that have described antimicrobial photodynamic therapy (APDT) effects on microorganisms. Several in vitro and in vivo studies have demonstrated the potential of APDT for treating endodontic, periodontal, and mucosal infections caused by bacteria as biofilms. Reactive oxygen species (ROS) are activated in the presence of oxygen by integrating a nontoxic photosensitizer (PS) with appropriate wavelength visible light. By causing irreversible damage to microorganisms, ROS induces some biological and photochemical events. Testing several wavelengths has been conducted to identify potential PS for APDT. A standard protocol is not yet available, and the current review summarizes findings from dental studies on APDT.

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Photodynamic inactivation of planktonic cultures and Streptococcus mutans biofilms for prevention of white spot lesions during orthodontic treatment: An in vitro investigation

Cited by 20 publications

References 50 publications

Effect of Photodynamic Therapy on Microorganisms Responsible for Dental Caries: A Systematic Review and Meta-Analysis

Effect of Photodynamic Therapy on Microorganisms Responsible for Dental Caries: A Systematic Review and Meta-Analysis

Effect of antibacterial photodynamic therapy on Streptococcus mutans plaque biofilm in vitro

The effect of photodynamic therapy in controlling the oral biofilm: A comprehensive overview

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