Photodissociation of laser-desorbed ions as a structure determination tool

Abstract

Laser desorption Fourier transform mass spectrometry (LD/FTMS) of porphyrins, metalloporphyrins, and alkaloids is used to investigate XeCl excimer laser photodissociation of trapped ions as an alternative to collision-induced dissociation for structure analysis purposes. It is shown that the presence of an appropriate metal enhances photodissociation and that in situ metal attachment during the laser desorption event may be a useful analytical strategy. Iron-, manganese-, and chromium-attached species are examined in order to assess the effect of metal upon the propensity for photodissociation with 308-nm excitation.

Efficiency of collisionally-activated dissociation and 193-nm photodissociation of peptide ions in fourier transform mass spectrometry

“…The energy deposited in photon abs orption is independent of mass. Laser photodissociation of ions is much more effective with stored ions [11,13,15,22) th an with high-velocity ions [23], and also with highenergy photons (e.g., 193 nm-6.4 eV), for obtaining sequence information for large peptides [11,13,15]. Surface-induced dissociation, pioneer ed by Mabud et a1.…”

Analysis of low molecular weight compounds by MALDI-FTICR-MS

“…In the last decades, LDI-MS has emerged as one of the most useful methods for analysis of non-volatile organic compounds. The improvements of attainable mass range and resolution for high mass molecules of LDI-FTICR-MS further extend its analytical applications [97], LDI-FTICR-MS has been applied in many fields, such as detection of polycyclic aromatic hydrocarbon (PAH) [98], division of isomers [99], polymer analysis [100], capture of radical ions [101], analysis of biological sample like plasma [102] and studies of some chemical process including gas-phase host-guest effects [103], photo-dissociation [104], and self-assembling complexes [105].…”

Knockout driven fragmentation of porphyrins

“…Several studies on MTPPs have been performed in order to investigate their stabilities under different forms of energetic processing, such as electron-impact (EI), [12][13][14][15][16] collisions with ions or atoms [17][18][19] or photoinduced dissociation (PID). 20,21 This type of experiments may contribute to a better understanding of damage to biomolecules e.g. in heavy ion therapy.…”