Analysis of low molecular weight compounds by MALDI-FTICR-MS

Wang, Haoyang; Xu, Can; Zhao, Zhixiong; He, Xin; Guo, Yinlong

doi:10.1016/j.jchromb.2011.03.037

Cited by 55 publications

(26 citation statements)

References 197 publications

(186 reference statements)

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“…The inhibition of enzyme activity by CSCs was analyzed by MALDI-FTMS. This method has been well-established by our laboratory (Xu et al 2008;Xu et al 2010;Wang et al 2011;Cai et al 2011), and this study is an expansion of its application. An important reason for using MALDI-FTMS to investigate the inhibition effect of CSCs on AChE activity is that CSCs are rather complex and dirty samples and the cell lysis solution is complex as well.…”

Section: Resultsmentioning

confidence: 99%

“…The method for determining enzyme activity based on MALDI-FTMS has been previously established by our laboratory (Xu et al 2008;Xu et al 2010;Wang et al 2011). Experiments were conducted using an Ionspec 4.7 T HisRes MALDI-FTMS (Ionspec, Irvine, CA, USA).…”

Section: Maldi-ftms Conditions and Analysismentioning

confidence: 99%

“…With various ionization techniques to monitor the contents of substrate and product, mass spectrometry offers great facilities for studying enzyme system (Northen et al 2008;Yamazaki and Takao 2008;Chen et al 2010;Vovk et al 2011). Several reports on determining enzyme activity by MALDI-FTMS have been made from our laboratory (Xu et al 2008;Xu et al 2010;Wang et al 2011;Cai et al 2011). MALDI-FTMS has obvious advantages to analyze enzyme inhibitor in complex raw and biological samples, attributed to its high speed, salt tolerance and low sample consumption.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Study of Effects of Cigarette Smoke Condensates on Acetylcholinesterase Activity in Human Lung Epithelial Cells by Matrix-Assisted Laser Desorption/Ionization-Fourier Transform Mass Spectrometry

Zhang

Guo

et al. 2012

Analytical Letters

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Matrix-Assisted Laser Desorption/Ionization-Fourier Transform Mass Spectrometry (MALDI-FTMS) is an emerging method for kinetic measurements and inhibitor screening of acetylcholinesterase (AChE). This method is accurate, reproducible, and was successfully applied to the analysis of various biosamples. Recent studies showed the important biological functions of acetylcholinesterase in many kinds of tumors caused by smoking; however, the effect of cigarette smoke condensates (CSCs) on AChE was unclear. Herein, the effects of CSCs on AChE activity in human lung epithelial cells were studied by MALDI-FTMS. The results showed that AChE activity in human lung epithelial cells could be inhibited by CSCs, and inhibition degree on AChE activity depended on the amount of CSCs. Owing to the close relationship of AChE to lung cancer caused by smoking, such findings provide information regarding the relation between the aggressiveness of lung tumor and cigarette smoking.

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Section: Resultsmentioning

confidence: 99%

Section: Maldi-ftms Conditions and Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Study of Effects of Cigarette Smoke Condensates on Acetylcholinesterase Activity in Human Lung Epithelial Cells by Matrix-Assisted Laser Desorption/Ionization-Fourier Transform Mass Spectrometry

Zhang

Guo

et al. 2012

Analytical Letters

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“…High resolution mass spectrometry has become an important tool in the qualitative and quantitative analysis of multiple compounds in complex matrices including herbal components [1,2], endogenous metabolites [3], peptides [4], and lipids [5]. However, a great challenge normally encountered in data processing is the detection of untargeted components as the ions of potential interest are largely masked by background noise.…”

Section: Introductionmentioning

confidence: 99%

Post acquisition data processing techniques for lipid analysis by quadrupole time-of-flight mass spectrometry

Xie

Liang

et al. 2012

Journal of Chromatography B

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“…Certain analogies between reaction mechanisms in the gas phase and solution have been known for a long time [1][2][3][4][5][6][7][8][9][10]19]. The reactivities and behaviors of ionic species in the gas phase and in solution can be correlated [12,[20][21][22], and there is a significant association between gas phase reactions and the analogous reactions in solution. Tandem mass spectrometry does not require large quantities of organic solvents and chemicals, and could be used as a high-throughput and green method to predict the chemical transformations of reactive compounds in solution.…”

Section: Introductionmentioning

confidence: 99%

Gas Phase Decarbonylation and Cyclization Reactions of Protonated N-Methyl-N-Phenylmethacrylamide and Its Derivatives Via an Amide Claisen Rearrangement

Wang

Zhu

et al. 2012

J. Am. Soc. Mass Spectrom.

Self Cite

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Gas phase decarbonylation and cyclization reactions of protonated N-methyl-N-phenylmethacrylamide and its derivatives (M·H + ) were studied by electrospray ionization-tandem mass spectrometry (ESI-MS/MS). MS/MS experiments of M·H + showed product ions were formed by loss of CO, which could only occur with an amide Claisen rearrangement. Mechanisms for the gas phase decarbonylation and cyclization reactions were proposed based on the accurate m/z measurements and MS/MS experiments with deuterated compounds. Theoretical computations showed the gas phase Claisen rearrangement was a major driving force for initiating gas phase decarbonylation and cyclization reactions of M·H + . Finally, the influence of different phenyl substituents on the gas phase Claisen rearrangement was evaluated. Electron-donating groups at the para-position of the phenyl moiety promoted the gas phase Claisen rearrangement to give a high abundance of fragment ions [M − CO + H] + . By contrast, electron-withdrawing groups on the phenyl moiety retarded the Claisen rearrangement, but gave a fragment ion at m/z 175 by loss of neutral radicals of substituents on the phenyl, and a fragment ion at m/z 160 by further loss of a methyl radical.

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Analysis of low molecular weight compounds by MALDI-FTICR-MS

Cited by 55 publications

References 197 publications

Study of Effects of Cigarette Smoke Condensates on Acetylcholinesterase Activity in Human Lung Epithelial Cells by Matrix-Assisted Laser Desorption/Ionization-Fourier Transform Mass Spectrometry

Study of Effects of Cigarette Smoke Condensates on Acetylcholinesterase Activity in Human Lung Epithelial Cells by Matrix-Assisted Laser Desorption/Ionization-Fourier Transform Mass Spectrometry

Post acquisition data processing techniques for lipid analysis by quadrupole time-of-flight mass spectrometry

Gas Phase Decarbonylation and Cyclization Reactions of Protonated N-Methyl-N-Phenylmethacrylamide and Its Derivatives Via an Amide Claisen Rearrangement

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