Efficiency of collisionally-activated dissociation and 193-nm photodissociation of peptide ions in fourier transform mass spectrometry

Williams, Evan R.; Furlong, Jorge; McLafferty, Fred W.

doi:10.1016/1044-0305(90)85003-5

Cited by 107 publications

(114 citation statements)

References 34 publications

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“…This feature is currently being evaluated among larger oligosaccharides derived from acidic proteoglycans. The advantage of combining UVPD or EPD with CID in MS n experiments for extensive structural analysis of such oligosaccharides, which has already proven to be successful for peptides [12][13][14][15], DNA [17,18], and glycans with higher energy photons [20 -22, 27], will also have to be evaluated. …”

Section: Resultsmentioning

confidence: 99%

“…UV and IR gas-phase photodissociation (i.e., ultraviolet photodissociation (UVPD) and infrared multiphoton dissociation (IRMPD) of positive and negative precursor ions also appears to be a very promising activation method for providing new or complementary structural information in CID. These techniques have been used for the analysis of biomolecules, such as proteins [6 -11], peptides [12][13][14][15][16], and nucleic acids [17][18][19]. In the case of permethylated and fixed-charge derivatized oligosaccharides, the 157 nm UVPD spectra yield intense cross ring fragment ions corresponding to a high-energy dissociation pathway,…”

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confidence: 99%

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Wavelength-tunable ultraviolet photodissociation (UVPD) of heparin-derived disaccharides in a linear ion trap

Racaud

Antoine

Joly

et al. 2009

J. Am. Soc. Mass Spectrom.

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A set of three heparin-derived disaccharide deprotonated ions was isolated in a linear ion trap and subjected to UV laser irradiation in the 220 -290 nm wavelength range. The dissociation yields of the deprotonated molecular ions were recorded as a function of laser wavelength. They revealed maximum absorption at 220 nm for the nonsulfated disaccharide, but centered at 240 nm for the sulfated species. The comparison of the fragmentation patterns between ultraviolet photodissociation (UVPD) at 240 nm and CID modes showed roughly the same distribution of fragment ions resulting from glycosidic bond cleavages. Interestingly, UVPD favored additional cross ring cleavages of A and X type ion series enabling easier sulfate group location. It also reduced small neutral losses ( T he major advances achieved in mass spectrometry have revolutionized the structural analysis of proteins and led to the emergence of proteomic strategies. Although software-assisted de novo sequencing of peptides resulting from unknown proteins has become a routine task, the structural assignment of complex N-and O-glycans is much less trivial. Unlike proteins accurately translated from a unique template that follows a universal code, a similar set of glycosyl transferases may, across different species, build oligosaccharides of different structure according to an a priori unpredictable chronological catalysis activity.Compared with N-and O-linked glycans, the issue of structural complexity is continuing to increase in the world of glycosaminoglycans. Glycosaminoglycans are linear polymerized disaccharidic repeating units containing hexuronic acids and hexosamines. Their essential roles in governing numerous biologic functions [1] have led many groups to focus their efforts on defining generic strategies based on mass spectrometry, to obtain the structural definition of these acidic oligosaccharides. In the case of oligosaccharides derived from heparin or heparane sulfate, both glucuronic and iduronic acids and glucosamine may be sulfated at their OH and NH 2 groups. The fragmentation behavior of heparin-derived oligosaccharides has proven to be difficult to predict. Sulfate groups often impede access to exhaustive structural information by collision induced dissociation (CID)-MS/MS. Indeed, when the charge state of the selected molecular ion is low, neutral SO 3 losses most often predominate in comparison with glycosidic backbone cleavages [2]. This situation can be improved by combining MS/MS data resulting from different charge states.Very recently, Amster and coworkers published outstanding results showing how promising the electron detachment dissociation (EDD) of deprotonated sulfated oligosaccharides can be in providing informative fragment ions [3]. In particular, they documented the discrimination between the C5 epimeric glucuronic and iduronic acids based on specific fragment ions [4]. As in CID mode, they also observed that SO 3 loss can be dramatically reduced when EDD is performed on the charge states where the carboxylate group is ...

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Section: Resultsmentioning

confidence: 99%

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confidence: 99%

Wavelength-tunable ultraviolet photodissociation (UVPD) of heparin-derived disaccharides in a linear ion trap

Racaud

Antoine

Joly

et al. 2009

J. Am. Soc. Mass Spectrom.

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“…Absorption of UV/vis photons by ions occurs by resonant photon absorption from the ground to an excited electronic state and commonly results in photodissociation of the excited ion. Photodissociation of peptides and small proteins has been achieved using 193 nm (6.42 eV) photons (Bowers et al, 1984;Bowers, Delbert, & McIver, 1986;Williams, Furlong, & McLafferty, 1990;Beu et al, 1993). However, photodissociation of larger proteins (ubiquitin ions, 8.6 kDa) resulted in formation of small, uninformative fragment ions (Beu et al, 1993).…”

Section: Multiphoton Dissociationmentioning

confidence: 99%

Activation of large lons in FT‐ICR mass spectrometry

Laskin

Futrell

2004

Mass Spectrometry Reviews

177

146

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The advent of soft ionization techniques, notably electrospray and laser desorption ionization methods, has enabled the extension of mass spectrometric methods to large molecules and molecular complexes. This both greatly extends the applications of mass spectrometry and makes the activation and dissociation of complex ions an integral part of these applications. This review emphasizes the most promising methods for activation and dissociation of complex ions and presents this discussion in the context of general knowledge of reaction kinetics and dynamics largely established for small ions. We then introduce the characteristic differences associated with the higher number of internal degrees of freedom and high density of states associated with molecular complexity. This is reflected primarily in the kinetics of unimolecular dissociation of complex ions, particularly their slow decay and the higher energy content required to induce decomposition-the kinetic shift (KS). The longer trapping time of Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) significantly reduces the KS, which presents several advantages over other methods for the investigation of dissociation of complex molecules. After discussing general principles of reaction dynamics related to collisional activation of ions, we describe conventional ways to achieve single-and multiple-collision activation in FT-ICR MS. Sustained off-resonance irradiation (SORI)-the simplest and most robust means of introducing the multiple collision activation process-is discussed in greatest detail. Details of implementation of this technique, required control of experimental parameters, limitations, and examples of very successful application of SORI-CID are described. The advantages of high mass resolving power and the ability to carry out several stages of mass selection and activation intrinsic to FT-ICR MS are demonstrated in several examples. Photodissociation of ions from small molecules can be effected using IR or UV/vis lasers and generally requires tuning lasers to specific wavelengths and/ or utilizing high flux, multiphoton excitation to match energy levels in the ion. Photodissociation of complex ions is much easier to accomplish from the basic physics perspective. The quasi-continuum of vibrational states at room temperature makes it very easy to pump relatively large amounts of energy into complex ions and infrared multiphoton dissociation (IRMPD) is a powerful technique for characterizing large ions, particularly biologically relevant molecules. Since both SORI-CID and IRMPD are slow activation methods they have many common characteristics. They are also distinctly different because SORI-CID is intrinsically selective (only ions that have a cyclotron frequency close to the frequency of the excitation field are excited), whereas IRMPD is not (all ions that reside on the optical path of the laser are excited). There are advantages and disadvantages to each technique and in many applications they complement each other. In contrast wi...

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“…A biomolecule sequence is generally obtained by tandem mass spectrometry (MS/MS) [5], in which a parent ion is first isolated from other ions of different mass-to-charge (m/z) ratio and then dissociated into sequence-specific fragment ions. The fragmentation technique (e.g., collision induced dissociation (CID) [6], infrared multiphoton dissociation (IRMPD) [7,8], blackbody infrared radiative dissociation (BIRD) [9,10], surface induced dissociation (SID) [11], or ultraviolet photodissociation [12,13]) as well as the nature of the parent ion charge (singly versus multiply charged, protonation versus metal cation attachment, cation versus anion, etc.) play key roles in determining the major fragmentation processes.…”

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confidence: 99%

Electron capture dissociation and infrared multiphoton dissociation of oligodeoxynucleotide dications

Håkansson

Hudgins

Marshall

et al. 2003

J. Am. Soc. Mass Spectrom.

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We report electron capture dissociation (ECD) and infrared multiphoton dissociation (IRMPD) of doubly protonated and protonated/alkali metal ionized oligodeoxynucleotides. Mass spectra following ECD of the homodeoxynucleotides polydC, polydG, and polydA contain w or d "sequence" ions. For polydC and polydA, the observed fragments are even-electron ions, whereas radical w/d ions are observed for polydG. Base loss is seen for polydG and polydA but is a minor fragmentation pathway in ECD of polydC. We also observe fragment ions corresponding to w/d plus water in the spectra of polydC and d(GCATGC). Although the structure of these ions is not clear, they are suggested to proceed through a pentavalent phosphorane intermediate. The major fragment in ECD of d(GCATGC) is a d ion. Radical a-or z-type fragment ions are observed in most cases. IRMPD primarily results in base loss, but backbone fragmentation is also observed. IRMPD provides more sequence information than ECD, but the spectra are more complex due to extensive base and water losses. It is proposed that the smaller degree of sequence coverage in ECD, with fragmentation mostly occurring close to the ends of the molecules, is a consequence of a mechanism in which the electron is captured at a P¢O bond, resulting in a negatively charged phosphate group. Consequently, at least two protons (or alkali metal cations) must be present to observe a w or d fragment ion, a requirement that is less likely for small fragments. , in which a parent ion is first isolated from other ions of different mass-to-charge (m/z) ratio and then dissociated into sequence-specific fragment ions. The fragmentation technique (e.g., collision induced dissociation (CID) [6], infrared multiphoton dissociation (IRMPD) [7,8], blackbody infrared radiative dissociation (BIRD) [9,10], surface induced dissociation (SID) [11], or ultraviolet photodissociation [12,13]) as well as the nature of the parent ion charge (singly versus multiply charged, protonation versus metal cation attachment, cation versus anion, etc.) play key roles in determining the major fragmentation processes.To date most attention has focused on the fragmentation reactions of even-electron ions, but electrospray ionization in combination with ion-ion reactions, ionelectron reactions, or ion-neutral reactions have opened up new opportunities to examine the fragmentation reactions of odd-electron ions. Examples include electron capture dissociation (ECD) of [M ϩ nH] nϩ ions of peptides and proteins [14,15], polymers [16,17] [24], and CID of a Cu(II)-amine-peptide complex [25]. In each case, the formation of the radical cation (or anion) results in new fragmentation pathways, which are complementary to those observed for even-electron fragmentation. For example, disulfide bond cleavage is observed as a major fragmentation pathway in ECD [26], whereas such cleavage is not observed in CID or IRMPD. In other instances, novel fragmentation chem-

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Efficiency of collisionally-activated dissociation and 193-nm photodissociation of peptide ions in fourier transform mass spectrometry

Cited by 107 publications

References 34 publications

Wavelength-tunable ultraviolet photodissociation (UVPD) of heparin-derived disaccharides in a linear ion trap

Wavelength-tunable ultraviolet photodissociation (UVPD) of heparin-derived disaccharides in a linear ion trap

Activation of large lons in FT‐ICR mass spectrometry

Electron capture dissociation and infrared multiphoton dissociation of oligodeoxynucleotide dications

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