Photobiomodulation therapy combined with carvedilol attenuates post-infarction heart failure by suppressing excessive inflammation and oxidative stress in rats

Abstract

The post-myocardial infarction heart failure (HF) still carries a huge burden since current therapy is unsuccessful to abrogate poor prognosis. Thus, new approaches are needed, and photobiomodulation therapy (PBMt) may be a way. However, it is not known whether PBMt added to a standard HF therapy provides additional improvement in cardiac remodeling in infarcted rats. This study sought to determine the combined carvedilol-drug and PBMt with low-level laser therapy value in HF. Rats with large infarcts were treated for 30 days. The functional fitness was evaluated using a motorized treadmill. Echocardiography and hemodynamic measurements were used for functional evaluations of left ventricular (LV). ELISA, Western blot and biochemical assays were used to evaluate inflammation and oxidative stress in the myocardium. Carvedilol and PBMt had a similar action in normalizing pulmonary congestion and LV end-diastolic pressure, attenuating LV dilation, and improving LV systolic function. Moreover, the application of PBMt to carvedilol-treated rats inhibited myocardial hypertrophy and improved +dP/dt of LV. PBMt alone prevented inflammation with a superior effect than carvedilol. Carvedilol and PBMt normalized 4-hydroxynonenal (a lipoperoxidation marker) levels in the myocardium. However, importantly, the addition of PBMt to carvedilol attenuated oxidized protein content and triggered a high activity of the anti-oxidant catalase enzyme. In conclusion, these data show that the use of PBMt plus carvedilol therapy results in a significant additional improvement in HF in a rat model of myocardial infarction. These beneficial effects were observed to be due, at least in part, to decreased myocardial inflammation and oxidative stress.

Post-resistance exercise photobiomodulation therapy has a more effective antioxidant effect than pre-application on muscle oxidative stress

“…The study protocol was approved by the Institutional Research Ethics Committee at the Uninove University, São Paulo-SP, Brazil (process: 8868250615). Based on rats' availability in our animal's facility, several protocols were performed using female rats that have been published [54,55]. Moreover, the landscape of female athletics has changed considerably in the last decades, in which female athletic participation in competitions has significantly increased worldwide [56].…”

“…2.6 Protein oxidationMeasurement of carbonyl groups added into proteins by oxidative reactions was evaluated with ABCAM kit AB178020 (Abcam, Cambridge, MA, USA) for an equal protein load (20 μg)[58,59]. Samples were loaded onto SDS-PAGE gels and DNP conjugated proteins were detected by western blotting using primary DNP antibody and HRP conjugated secondary antibody.…”

A common oral pathogen Porphyromonas gingivalis induces myocarditis in rats

“…The oxidized protein content was analysed by Western blot with kit from Abcam (ref. : ab178020, Abcam, Cambridge, MA) in a homogenate of the LV containing 10 μg of protein (de Oliveira et al, 2018; Grandinetti et al, 2019). Samples were submitted to electrophoresis in SDS‐polyacrylamide (12%) and then the proteins were transferred to polyvinylidene fluoride membranes.…”

Pharmacodynamic Evaluation of Shenfu Injection in Rats With Ischemic Heart Failure and Its Effect on Small Molecules Using Matrix-Assisted Laser Desorption/Ionization–Mass Spectrometry Imaging

“…The surviving rats with ligation of the left anterior descending artery were randomly divided into the model (MOD), CAR [10 mg/kg (Grandinetti et al, 2019), 6 ml/kg], and Shenfu injection groups [L, M, and H groups receiving Shenfu injection at concentrations of 3, 6, and 12 ml/kg, respectively (corresponding to clinical dosages of 36, 72, and 144 ml/70 kg, respectively)] 48 h after surgery. The CAR group (n = 9) received the drug intragastrically, whereas the L, M, and H groups received Shenfu injection intraperitoneally.…”