Photoacoustic Imaging in Tissue Engineering and Regenerative Medicine

Shrestha, Binita; DeLuna, Frank; Anastasio, Mark A.; Ye, Jing Yong; Brey, Eric M.

doi:10.1089/ten.teb.2019.0296

Cited by 30 publications

(14 citation statements)

References 281 publications

(308 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In the future, novel imaging technologies, such as multi-photon fluorescence microscopy [ 105 , 106 ] and photoacoustic imaging [ 107 , 108 ], may markedly improve our knowledge of the functionality of the microvascular networks in non-unions by the direct measurement of oxygen saturation within the callus tissue. In combination with advanced immunohistochemical staining methods and molecular biological approaches it may be possible to identify and investigate potential growth factors and mediators involved in the pathology of vascular dysfunction during non-union formation.…”

Section: Future Perspectivesmentioning

confidence: 99%

The vascularization paradox of non-union formation

Menger

Laschke

Nüssler³

et al. 2022

Angiogenesis

View full text Add to dashboard Cite

Despite major research efforts to elucidate mechanisms of non-union formation, failed fracture healing remains a common complication in orthopedic surgery. Adequate vascularization has been recognized as a crucial factor for successful bone regeneration, as newly formed microvessels guarantee the supply of the callus tissue with vital oxygen, nutrients, and growth factors. Accordingly, a vast number of preclinical studies have focused on the development of vascularization strategies to stimulate fracture repair. However, recent evidence suggests that stimulation of blood vessel formation is an oversimplified approach to support bone regeneration. This review discusses the role of vascularization during bone regeneration and delineates a phenomenon, for which we coin the term “the vascularization paradox of non-union-formation”. This view is based on the results of a variety of experimental studies that suggest that the callus tissue of non-unions is indeed densely vascularized and that pro-angiogenic mediators, such as vascular endothelial growth factor, are sufficiently expressed at the facture site. By gaining further insights into the molecular and cellular basis of non-union vascularization, it may be possible to develop more optimized treatment approaches or even prevent the non-union formation in the future.

show abstract

Section: Future Perspectivesmentioning

confidence: 99%

The vascularization paradox of non-union formation

Menger

Laschke

Nüssler³

et al. 2022

Angiogenesis

View full text Add to dashboard Cite

show abstract

“…Аналогичный подход был опробован на композитных каркасах с одностенными углеродными нанотрубками (ОУНТ). Показано, что комбинация PLGA/ОУНТ приводила к еще более высокой экспрессии генов и пролиферации клеток для формирования новой мышечной ткани, по сравнению с композитным носителем из PLGA и МУНТ [77]. Предполагается, что такая клеточная активность является следствием повышенной экспрессии трансмембранных клеточных рецепторов -интегринов, что может быть вызвано топографическими особенностями ОУНТ.…”

Section: композитные каркасы с углеродными нанотрубкамиunclassified

“…Предполагается, что такая клеточная активность является следствием повышенной экспрессии трансмембранных клеточных рецепторов -интегринов, что может быть вызвано топографическими особенностями ОУНТ. Такая активность является ключевым элементом для лучшего взаимодействия полимерного каркаса с биологическими компонентами [77].…”

Section: композитные каркасы с углеродными нанотрубкамиunclassified

New-generation osteoplastic materials based on biological and synthetic matrices

Lykoshin

Зайцев

Костромина

et al. 2021

Fine Chem. Technol.

View full text Add to dashboard Cite

Objectives. The purpose of this analytical review is to evaluate the market for osteoplastic materials and surgical implants, as well as study the features of new-generation materials and the results of clinical applications.Methods. This review summarizes the volumes of research articles presented in the electronic database PubMed and eLIBRARY. A total of 129 scientific articles related to biological systems, calcium phosphate, polymer, and biocomposite matrices as carriers of pharmaceutical substances, primary recombinant protein osteoinductors, antibiotics, and biologically active chemical reagents were analyzed and summarized. The search depth was 10 years.Results. Demineralized bone matrix constitutes 26% of all types of osteoplastic matrices used globally in surgical osteology, which includes neurosurgery, traumatology and orthopedics, dentistry, and maxillofacial and pediatric surgery. Among the matrices, polymer and biocomposite matrices are outstanding. Special attention is paid to the possibility of immobilizing osteogenic factors and target pharmaceutical substances on the scaffold material to achieve controlled and prolonged release at the site of surgical implantation. Polymeric and biocomposite materials can retard the release of pharmaceutical substances at the implantation site, promoting a decrease in the toxicity and an improvement in the therapeutic effect. The use of composite scaffolds of different compositions in vivo results in high osteogenesis, promotes the initialization of biomineralization, and enables the tuning of the degradation rate of the material.Conclusions. Osteoplastic materials of various compositions in combination with drugs showed accelerated regeneration and mineralization of bone tissue in vivo, excluding systemic side reactions. Furthermore, although some materials have already been registered as commercial drugs, a plethora of unresolved problems remain. Due to the limited clinical studies of materials for use on humans, there is still an insufficient understanding of the toxicity of materials, time of their resorption, speed of drug delivery, and the possible long-term adverse effects of using implants of different compositions.

show abstract

“…With recent advances in high-speed scanning, PAM systems with B-scan rates as high as a few hundred hertz have been actively explored [21] , [22] , [23] , [24] . PAM’s ability to perform high-speed and high-resolution imaging (within a few seconds per cubic millimeters and a resolution of a few micrometers) has enabled hemodynamic monitoring studies such as those on external stimulation, drug responses, vascular diseases, and regenerative medicine [25] , [26] , [27] , [28] .…”

Section: Introductionmentioning

confidence: 99%