Photoacoustic Imaging: A Novel Tool for Detecting Carotid Artery Thrombosis in Mice

Li, Bing; Cong, Fengyu; Ma, Genshan; Fan, Quli; Yao, Yuyu

doi:10.1159/000477631

Cited by 12 publications

(6 citation statements)

References 26 publications

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“…Two or three drops of saline were applied to the vessel to avoid drying up. A piece of filter paper saturated with 20% (w/v) FeCl 3 solution was placed on the artery and incubated for 3 min (Liang et al, 2015b ; Li et al, 2017 ). After incubation the artery was rinsed with saline.…”

Section: Methodsmentioning

confidence: 99%

VAMP3 and SNAP23 as Potential Targets for Preventing the Disturbed Flow-Accelerated Thrombus Formation

Zhu

Jiang

Liu

et al. 2020

Front. Cell Dev. Biol.

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Disturbed blood flow has been recognized to promote platelet aggregation and thrombosis via increasing accumulation of von Willebrand factor (VWF) at the arterial post-stenotic sites. The mechanism underlying the disturbed-flow regulated endothelial VWF production remains elusive. Here we described a mouse model, in which the left external carotid artery (LECA) is ligated to generate disturbed flow in the common carotid artery. Ligation of LECA increased VWF accumulation in the plasma. Carotid arterial thrombosis was induced by ferric chloride (FeCl 3) application and the time to occlusion in the ligated vessels was reduced in comparison with the unligated vessels. In vitro, endothelial cells were subjected to oscillatory shear (OS, 0.5 ± 4 dynes/cm 2) or pulsatile shear (PS, 12 ± 4 dynes/cm 2). OS promoted VWF secretion as well as the cell conditioned media-induced platelet aggregation by regulating the intracellular localization of vesicle-associated membrane protein 3 (VAMP3) and synaptosomal-associated protein 23 (SNAP23). Disruption of vimentin intermediate filaments abolished the OS-induced translocation of SNAP23 to the cell membrane. Knockdown of VAMP3 and SNAP23 reduced the endothelial secretion of VWF. Systemic inhibition of VAMP3 and SNAP23 by treatment of mice with rapamycin significantly ameliorated the FeCl 3-induced thrombogenesis, whereas intraluminal overexpression of VAMP3 and SNAP23 aggravated it. Our findings demonstrate VAMP3 and SNAP23 as potential targets for preventing the disturbed flow-accelerated thrombus formation.

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Section: Methodsmentioning

confidence: 99%

VAMP3 and SNAP23 as Potential Targets for Preventing the Disturbed Flow-Accelerated Thrombus Formation

Zhu

Jiang

Liu

et al. 2020

Front. Cell Dev. Biol.

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“…In (38), OA was employed to detect thrombosis of the carotid artery in a corresponding animal model (Figure 4A). Carotid artery thrombosis was induced by an iatrogenic chemical injury via the application of a filter paper soaked with 20% FeCl 3 around the vessel wall after the surgical exposure of the carotid artery.…”

Section: Small Animal Modelsmentioning

confidence: 99%

“…The white arrows indicate the locations of the thrombi in the FeCl3-treated mice. Adapted with permission from(38). (B) Optoacoustic (1,064 nm)-ultrasound overlays of the carotid arteries/plaques of a mouse before (up) and 24 h after (middle and down) the intravenous injection of a molecular probe (PBD-CD36 NP) targeting inflammation.…”

mentioning

confidence: 99%

Optoacoustic biomarkers of lipids, hemorrhage and inflammation in carotid atherosclerosis

Karlas,

Fasoula,

Kallmayer

et al. 2023

Front. Cardiovasc. Med.

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Imaging plays a critical role in exploring the pathophysiology and enabling the diagnostics and therapy assessment in carotid artery disease. Ultrasonography, computed tomography, magnetic resonance imaging and nuclear medicine techniques have been used to extract of known characteristics of plaque vulnerability, such as inflammation, intraplaque hemorrhage and high lipid content. Despite the plethora of available techniques, there is still a need for new modalities to better characterize the plaque and provide novel biomarkers that might help to detect the vulnerable plaque early enough and before a stroke occurs. Optoacoustics, by providing a multiscale characterization of the morphology and pathophysiology of the plaque could offer such an option. By visualizing endogenous (e.g., hemoglobin, lipids) and exogenous (e.g., injected dyes) chromophores, optoacoustic technologies have shown great capability in imaging lipids, hemoglobin and inflammation in different applications and settings. Herein, we provide an overview of the main optoacoustic systems and scales of detail that enable imaging of carotid plaques in vitro, in small animals and humans. Finally, we discuss the limitations of this novel set of techniques while investigating their potential to enable a deeper understanding of carotid plaque pathophysiology and possibly improve the diagnostics in future patients with carotid artery disease.

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“…By measuring the optical absorption of hemoglobin, Li et al reported the detection of artery thrombosis in mice. 134 Red blood cells (RBCs) in fresh clot formation generate higher PA signal than old clots. As the clot ages, it retracts forcing RBCs and other content out of the clot 135 resulting in a reduced PA signal.…”

Section: Vascular Imagingmentioning

confidence: 99%

Photoacoustic Imaging in Tissue Engineering and Regenerative Medicine

Shrestha

DeLuna

Anastasio

et al. 2020

Tissue Engineering Part B: Reviews

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Several imaging modalities are available for investigation of the morphological, functional, and molecular features of engineered tissues in small animal models. While research in tissue engineering and regenerative medicine (TERM) would benefit from a comprehensive longitudinal analysis of new strategies, researchers have not always applied the most advanced methods. Photoacoustic imaging (PAI) is a rapidly emerging modality that has received significant attention due to its ability to exploit the strong endogenous contrast of optical methods with the high spatial resolution of ultrasound methods. Exogenous contrast agents can also be used in PAI for targeted imaging. Applications of PAI relevant to TERM include stem cell tracking, longitudinal monitoring of scaffolds in vivo, and evaluation of vascularization. In addition, the emerging capabilities of PAI applied to the detection and monitoring of cancer and other inflammatory diseases could be exploited by tissue engineers. This article provides an overview of the operating principles of PAI and its broad potential for application in TERM.

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Photoacoustic Imaging: A Novel Tool for Detecting Carotid Artery Thrombosis in Mice

Cited by 12 publications

References 26 publications

VAMP3 and SNAP23 as Potential Targets for Preventing the Disturbed Flow-Accelerated Thrombus Formation

VAMP3 and SNAP23 as Potential Targets for Preventing the Disturbed Flow-Accelerated Thrombus Formation

Optoacoustic biomarkers of lipids, hemorrhage and inflammation in carotid atherosclerosis

Photoacoustic Imaging in Tissue Engineering and Regenerative Medicine

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