Photo-patterning PEG-based hydrogels for neuronal engineering

Berkovitch, Yulia; Yelin, Dvir; Seliktar, Dror

doi:10.1016/j.eurpolymj.2015.07.014

Cited by 21 publications

(29 citation statements)

References 46 publications

(61 reference statements)

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“…Thiolation of gelatin (Gelatin A, Porcine skin, Sigma) was accomplished using succinimidylacetyl‐thioacetate (SATA, Pierce, Illinois, USA) based on the protocols described by Chen, Noah, Pallua and Steffens () and further modified according to Berkovitch et al (). Briefly, gelatin was dissolved in 150‐mM PBS with 8‐M urea at a concentration of 7 mg/ml.…”

Section: Methodsmentioning

confidence: 99%

“…All treatments were compared with saline and unoperated (wild‐type) controls (Groups VII and VIII, respectively). To fabricate the NGCs, each hydrogel precursor solution was injected into a long silicone tube mold with a 1.5‐ or 2‐mm inner diameter, and cross‐linked according to methods that were previously described (Berkovitch et al, ).…”

Section: Methodsmentioning

confidence: 99%

“…Microchannels were ablated within the hydrogels using a commercial laser microdissector (P.A.L.M. MicroBeam system, Carl Zeiss MicroImaging GmbH, Bernried, Germany) set with a 20× objective and motorized microscope stage (Berkovitch et al, ; Sarig‐Nadir et al, ). Using a 1.35‐mm beam diameter, the irradiance of the pulse train (355 nm wavelength, 1 ns pulse duration, 3pJ per pulse, and 100 Hz repetition rate) at the 2.6 μm diameter focal spot was approximately 4.5 × 10 4 W/cm 2 .…”

Section: Methodsmentioning

confidence: 99%

“…The synthetic PEG component provides control over mechanical properties and degradation characteristics (Berdichevski, Shachaf, Wechsler, & Seliktar, ; Berkovitch & Seliktar, ; Dikovsky, Bianco‐Peled, & Seliktar, ; Gonen‐Wadmany, Goldshmid, & Seliktar, ). In addition, microchannels were inscribed using a photo‐ablation technique in order to target reinnervation with limiting dispersion of regenerating axons (Berkovitch, Yelin, & Seliktar, ; Sarig‐Nadir et al, ). The combination of controlled bioactivity, controlled physical characteristics, and uniaxial spatial patterns may ultimately provide us with an ideal NGC for regenerating peripheral nerves.…”

Section: Introductionmentioning

confidence: 99%

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Hydrogel composition and laser micropatterning to regulate sciatic nerve regeneration

Berkovitch

Cohen

Peled

et al. 2018

J Tissue Eng Regen Med

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Treatment of peripheral nerve injuries has evolved over the past several decades to include the use of sophisticated new materials endowed with trophic and topographical cues that are essential for in vivo nerve fibre regeneration. In this research, we explored the use of an advanced design strategy for peripheral nerve repair, using biological and semi-synthetic hydrogels that enable controlled environmental stimuli to regenerate neurons and glial cells in a rat sciatic nerve resection model. The provisional nerve growth conduits were composed of either natural fibrin or adducts of synthetic polyethylene glycol and fibrinogen or gelatin. A photo-patterning technique was further applied to these 3D hydrogel biomaterials, in the form of laser-ablated microchannels, to provide contact guidance for unidirectional growth following sciatic nerve injury. We tested the regeneration capacity of subcritical nerve gap injuries in rats treated with photo-patterned materials and compared these with injuries treated with unpatterned hydrogels, either stiff or compliant. Among the factors tested were shear modulus, biological composition, and micropatterning of the materials. The microchannel guidance patterns, combined with appropriately matched degradation and stiffness properties of the material, proved most essential for the uniform tissue propagation during the nerve regeneration process.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Hydrogel composition and laser micropatterning to regulate sciatic nerve regeneration

Berkovitch

Cohen

Peled

et al. 2018

J Tissue Eng Regen Med

Self Cite

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show abstract

“…However, neither gel supported rapid infi ltration into the guidance channels like the fi brinogenbased hydrogel. This was attributed to the contact guidance and opportunities for cellular adhesion associated with fi brinogen (Berkovitch et al 2015 ).…”

Section: Photoablationmentioning

confidence: 99%