Phosphorus Metabolites in Different Muscles of the Rat Leg by31P Image-SelectedIn Vivo Spectroscopy

Madhu, B; Lagerwall, K.; Soussi, B.

doi:10.1002/(sici)1099-1492(199612)9:8<327::aid-nbm437>3.0.co;2-o

Cited by 11 publications

References 9 publications

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Metabolism and Metabolomics by MRS

Chung

Madhu

Griffiths

2015

eMagRes

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The main use of MRS is for the assessment of metabolism in vivo; that is because it has the unique ability to monitor metabolism noninvasively without the use of ionizing radiation. In recent years MRS has also become widely used for metabolomics, the science that aspires to monitor the metabolomethe totality of small-molecule metabolites in an organism, a cell or a disease. This chapter discusses the properties of MRS that make it suitable for metabolomic studies and the reasons why they are mainly performed ex vivo, as well as the methods used for preparing samples, performing the MRS and analyzing the resulting data. Further sections discuss MRS detection of metabolic aspects of the common cellular processes apoptosis, necrosis and autophagy. A final section summarizes metabolic studies of some diseases by MRS: cancer, cardiovascular disease, neurological and neurodegenerative diseases, and muscle disease.

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Metabolism and Metabolomics by MRS

Chung

Madhu

Griffiths

2015

eMagRes

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Mapping fiber orientation in human muscle by proton MR spectroscopic imaging

Vermathen

Boesch

Kreis

2003

Magnetic Resonance in Med

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Proton magnetic resonance spectroscopic imaging ( 1 H-MRSI) was used to determine muscle fiber orientations in human calf muscles. The method is based on the fact that some resonances show orientation-dependent dipolar splitting, caused by incomplete motional averaging. This leads to proton spectra that depend strongly on the angle between muscle fibers and the magnetic field B 0 . The orientation-dependent dipolar splittings were mapped using a fit with a basis set of predefined coupling patterns reflecting the fiber orientation. The fitted coupling patterns were displayed as images and assigned to different muscles based on segmented MR images. They showed gross differences in fiber orientation between some muscles, including m. soleus and m. tibialis anterior, for all subjects. In addition, smaller but significant differences between subjects were detected, which could be due to localization differences or real interindividual differences. Since dipolar splitting affects metabolite intensities, it is important to take this effect into account when calculating metabolite concentrations from MR spectra in muscle tissue. Spatial maps of the MR signals of trimethyl-ammonium groups and creatine/ phosphocreatine revealed significant differences in intensity between muscles. Magn Reson Med 49:424 -432, 2003.

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