Phospholipids of<i>Trichophyton rubrum</i>

Das, Sumantra; Banerjee, Ayan

doi:10.1080/00362177485380411

Cited by 22 publications

(5 citation statements)

References 11 publications

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“…Yeast isolates were more active for the production of the two enzymes. Another study [9] reported that over 75% of strains of M. gypseum, M. canis, E.fEoccosum and T. mentagrophytes are lipase producers, while only one strain of T. rubrum secreted both enzymes [8]. The role of these enzymes is suggested to maintain the function of the fungal cell membrane and to aid in the invasion of host tissue [8].…”

Section: ~~-mentioning

confidence: 99%

Extracellular enzyme activities of dermatophytes and yeast isolates on solid media

Muhsin

Aubaid

Al-Duboon

1997

Mycoses

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A total of 123 isolates of 14 species of dermatophytes and yeasts were screened for the activity of five extracellular enzymes including elastase, keratinase, protease (gelatinase), lipase and phospholipase, by using solid media. The optimal production and activity of each enzyme was determined with regard to different pH and temperatures. Keratinase activity was high with all the tested fungi with exception of Malassezia furfur. Protease (gelatinase) was produced only by dermatophytes. Elastase was secreted by three dermatophytes viz. Microsporum gypseum, Trichophyton mentagrophytes var. mentagrophytes and T. verrucosum, whereas lipase and phospholipase were detected in all the species except T. violaceum.

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Section: ~~-mentioning

confidence: 99%

Extracellular enzyme activities of dermatophytes and yeast isolates on solid media

Muhsin

Aubaid

Al-Duboon

1997

Mycoses

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“…An isolate of T. rubrum, obtained from the School of Tropical Medicine, Calcutta [5] was maintained on Sabouraud's agar slants. For enzyme studies the fungus was grown in 50 ml glucose-peptone broth (glucose, 40 g l-~; Bacteriological peptone, Oxoid L37; 10 gl-~; pH 5-4) in 250 ml Erlenmeyer flasks without agitation at 34°C_+ I°C.…”

Section: Fungus and Growth Mediamentioning

confidence: 99%

Purification and partial characterization of an exocellular proteinase fromTrichophyton rubrum

Sanyal

Das

Banerjee³

1985

Med Mycol

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An exocellular proteinase produced by Trichophyton rubrum in a glucose-peptone broth was purified from lyophilized and dialysed culture filtrate of the dermatophyte by Sephadex G-100 gel filtration and preparative polyacrylamide gel electrophoresis. The purified enzyme was a homogeneous protein of molecular weight 34 700 and it could hydrolyse azoalbumin, casein, bovine serum albumin, a-N-benzoyl-L-arginine ethyl ester and p-toluenesulfonyl-L-arginine methyl ester but not N-benzoyl-L-tyrosine ethyl ester, a-N-benzoyl-DL-arginine-p-nitroanilide and keratin. The enzyme showed an alkaline pH optimum and was not activated by divalent metal ions but inhibited strongly by phenylmethylsulfonylfluoride. Thus the enzyme was identified as an alkaline serine proteinase. Exocellular and mycelial proteinases occur commonly in dermatophytes of the genus Trichophyton, including T. rubrum. These enzymes could play a prominent role not only in growth and multiplication (ensuring nutrient supply from the proteinaceous components of the skin, hair or nail) but also in the infection of the host tissue. Minocha et al. [18] reported on the role of proteolytic enzymes in the manifestation of inflammatory reactions in hosts with dermatophyte infections. Exo-or endocellular proteinases have been purified to homogeneity for detailed enzymatic studies from different dermatophytes, e.g.T, granulosum [8], T. mentagrophytes [32,33,34], Microsporum gypseum [11,24] and M. canis [25].Several groups of workers demonstrated the presence of proteinases and peptidases in the cells or culture filtrate of T. rubrum [2,3,4,17,26]. The exo-and endocellular proteinase activity of this fungus showed multiple pH optima and probably represented a mixture of closely related enzymes. Accumulation of proteolytic activity in the culture filtrate of T. rubrum was stimulated if keratin was added as growth substrate [2,17] and suppressed by glucose, various carbohydrates and amino acids [17]. However, no successful attempts were made for the purification of these enzymes from T. rubrum though a limited degree of purification has been reported by Chattaway et al. [2] and Meevootisom & Niederpruem [17].

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“…Extracellular DNase activity was described as required for the normal progression of Group A Streptococcus infection by enhancing evasion of the innate immune system 25 . The highest values of elastase and gelatinase activity registered in T. mentagrophytes and the highest lipase activity in M. canis isolates from animals with skin lesions suggest that these enzymes might play a role in the occurrence of lesions on rabbits as previously reported for the same dermatophytes in both humans and pet animals 15,16,21,26 …”

Section: Discussionmentioning

confidence: 70%

“…25 The highest values of elastase and gelatinase activity registered in T. mentagrophytes and the highest lipase activity in M. canis isolates from animals with skin lesions suggest that these enzymes might play a role in the occurrence of lesions on rabbits as previously reported for the same dermatophytes in both humans and pet animals. 15,16,21,26 Finally, our data indicate that T. mentagrophytes and M. canis isolated from rabbit hair coat produce enzymes with different levels of expression depending upon factors related to the host. The relationship between each enzyme and the occurrence of skin lesions varies on whether the infection is caused by T. mentagrophytes or M. canis.…”

Section: Discussionmentioning

confidence: 72%

Enzymatic activity of Microsporum canis and Trichophyton mentagrophytes from breeding rabbits with and without skin lesions

Cafarchia

Figueredo

Coccioli

et al. 2011

Mycoses

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Microsporum canis and Trichophyton mentagrophytes are zoophilic dermatophytes which can cause skin infections in animals and humans. The clinical expression of this infection strongly varies depending on host, fungal species as well as enzyme production. No comparative studies are available on the enzymatic activities of M. canis and T. mentagrophytes isolated from breeding rabbits. Thus, the aim of this work was to assess the capability of M. canis and T. mentagrophytes isolated from rabbits both with and without lesions in producing different enzymes. The relationship of dermatophyte enzymatic activities and presence/absence of skin lesions has also been investigated. A total of 260 isolates of T. mentagrophytes and 25 isolates of M. canis sampled both from healthy and lesioned skin of rabbits, as well as from air samples of positive farms were examined. The results showed that T. mentagrophytes and M. canis from rabbits produce different enzymes. However, only elastase and gelatinase were linked to the appearance of lesions in T. mentagrophytes infections, whereas lipase in those by M. canis.

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Phospholipids ofTrichophyton rubrum

Cited by 22 publications

References 11 publications

Extracellular enzyme activities of dermatophytes and yeast isolates on solid media

Extracellular enzyme activities of dermatophytes and yeast isolates on solid media

Purification and partial characterization of an exocellular proteinase fromTrichophyton rubrum

Enzymatic activity of Microsporum canis and Trichophyton mentagrophytes from breeding rabbits with and without skin lesions

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