Phospholipid profiles of Penicillium chrysogenum in different scales of fermentations

Abstract

Optimizing industrial‐scale fermentation for Penicillium chrysogenum is of significance for increasing commercial production of antibiotics. Lipidomics could be a valuable tool for the investigation in the behaviors of P. chrysogenum in realistic industrial environments. In this work, the phospholipid (PL) profiles of an industrial strain of P. chrysogenum were compared in different scale processes. PL was performed on LC/ESI/MSn system. It was found that industrial P. chrysogenum cells absorbed significant amounts of exogenous saturated and (poly) unsaturated fatty acids (PUFAs) from feedstock and incorporated them into their cell membranes during two fermentations. Results showed that the PL species that contain the PUFAs, that is, linolenic acid and hexadecadienoic acid, were quite variable between pilot and industrial scales of fermentations. Higher levels of PUFA‐containing PLs in rapid‐ and linear‐growth stages during industrial fermentation implied the occurrence of dramatic variations in cell membrane fluidity during these periods. It was speculated that this behavior was due to impacts of multiple physical and chemical factors present in the fermentation environment during this large‐scale fed‐batch process. The identified PUFA‐containing PLs could be used as valuable biomarkers for optimizing industrial‐scale fermentation for P. chrysogenum.

Metabolômica Microbiana: Inovações E Aplicações

“…79 Análises de perfis de fosfolípidios de Penicillium chrysogenum foram empregadas para avaliar essa variação em diferentes condições de cultivo do micro--organismo. 80 A GC-MS também foi empregada na análise de ácidos graxos submetidos à derivatização 81,82 tanto para avaliações de perfil lipídico 82,83 quanto para identificações específicas. [84][85][86][87] O estudo da produção metabólica em diferentes meios de cultura foi realizado para os micro-organismos Escherichia coli e Saccharomyces cerevisiae onde GC-TOF-MS (do inglês, Gas Chromatography -Time of Flight Mass Spectrometry) foi utilizada na identificação e quantificação dos metabólitos intracelulares.…”

Lipidomic Analysis: From Archaea to Mammals

“…The lipidomic profile of Penicillium chrysogenum was determined by LC–ESI‐MS n (Qiao, Lu, Cao, Chen, & Yuan, ) in industrial‐scale fermentations. During the fermentation, cells were shown to absorb significant amounts of exogenous saturated FA and polyunsaturated fatty acids (PUFA are FA with methylene‐interrupted double bonds, that is, with two or more double bonds of the cis‐configuration separated by a single methylene group) from the feedstock, and incorporate them into their cell membranes.…”

Industrial biotechnology in Asia — recent advances in biofuels and biorefinery

“…Therefore, this special issue starts with these two reviews on both in vitro and in vivo bioproduction systems. They are followed by seven original research articles with topics ranging from dynamic control of biosynthesis by using synthetic imolecular biosensors (Teo et al) ; engineering of industrial Saccharomyces cerevisiae strain for a more efficient production of ethanol over analysis of industrial scale (Yuan et al) ; fermentation by phosphalipid profiling using lipidomics (Qiao et al 2013) ; downstream processing for chemicals (Peng et al and Song et al) , fuels (Sun et al) , and proteins (Yoshimoto et al) . As highlighted in this issue, the work of Teo et al well demonstrates the potential of synthetic biology in engineering bioproduction systems.…”