Phospholipid composition of blood plasma, erythrocytes, and "ghosts" in sickle cell disease.

Schwarz, Hans‐Peter; Dahlke, Marion; Dreisbach, L.

doi:10.1093/clinchem/23.9.1548

Cited by 30 publications

(10 citation statements)

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“…The total concentration of all phospholipids in human plasma is about 1.6–3.0 mg/mL 15. PC is the most abundant class of phospholipids in human plasma, accounting for about 60–70% of the total phospholipids16, 17 and the other abundant classes are SM, PE, PI, lysoPC, PS and PG listed in the order of decreasing concentration 17…”

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confidence: 99%

Phospholipids in liquid chromatography/mass spectrometry bioanalysis: comparison of three tandem mass spectrometric techniques for monitoring plasma phospholipids, the effect of mobile phase composition on phospholipids elution and the association of phospholipids with matrix effects

Xia

Jemal

2009

Rapid Comm Mass Spectrometry

195

113

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Because plasma phospholipids may cause matrix effects in bioanalytical liquid chromatography/tandem mass spectrometry (LC/MS/MS) methods, it is important to establish optimal mass spectrometric techniques to monitor the fate of phospholipids during method development and application. We evaluated three MS/MS techniques to monitor phospholipids using positive and negative electrospray ionization (ESI). The first technique is based on using positive precursor ion scan of m/z 184, positive neutral loss scan of 141 Da and negative precursor ion scan of m/z 153. The second technique is based on using class-specific positive and negative selected reaction monitoring (SRM) transitions to monitor class-representative phospholipids. The third technique, previously reported, utilizes in-source collision-induced dissociation (CID)-based positive SRM of m/z 184 --> 184. We recommend the all-inclusive technique 1 for use in qualitative assessment of all classes of phospholipids and technique 2 for use in quantitative assessment of class-representative phospholipids. Secondly, we evaluated the elution behaviors of the plasma phospholipids under different reversed-phase mobile phase conditions. The phospholipid-eluting strength of a mobile phase was mainly dependent on the type and amount (%) of the organic eluent and the strength increased in the order of methanol, acetonitrile and isopropyl alcohol. Under the commonly used gradient and isocratic elution schemes in LC/MS/MS bioanalysis, not all the phospholipids are eluted off the column. Thirdly, we investigated the association between phospholipids and matrix effects in positive and negative ESI using basic, acidic and neutral analytes. While the phospholipids caused matrix effects in both positive and negative ESI, the extent of ionization suppression was analyte-dependent and was inversely related to the retention factor and broadness of the phospholipids peaks. The lysophospholipids which normally elute earlier in reversed-phase chromatography are more likely to cause matrix effects compared to the later-eluting phospholipids in spite of the larger concentrations of the latter in plasma.

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confidence: 99%

Phospholipids in liquid chromatography/mass spectrometry bioanalysis: comparison of three tandem mass spectrometric techniques for monitoring plasma phospholipids, the effect of mobile phase composition on phospholipids elution and the association of phospholipids with matrix effects

Xia

Jemal

2009

Rapid Comm Mass Spectrometry

195

113

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“…Since erythrocyte and plasma lipids are continually exchanged through active and passive processes (Shohet 1977) there may be alterations in erythrocyte membrane lipids. There are a number of other observations that suggest an altered erythrocyte membrane in sickle cell anaemia ; sickled erythrocytes have increased potassium fluxes (Tosteson, Carlsen & Dunham 1955), increased stromal lipid concentration (Westerman et al 1964, Erickson et al 1937, Akinyanju & Akinyanju, 1976, Schwarz, Dahlke & Dreisbach 1977, increased calcium accumulation (Eaton et al 1973), and irreversibly sickled cells remain sickled even upon reoxygenation of the haemoglobin (Bertles & Milner 1968).…”

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confidence: 99%

Plasma and erythrocyte lipids in sickle cell anaemia

Sasaki

Waterman

Buchanan

et al. 2008

Clinical &Amp; Laboratory Haematology

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Summary. The content and composition of plasma and erythrocyte lipids from individuals having sickle cell anaemia has been determined. The plasma contains a significantly reduced content of both cholesterol and phospholipid while the ratio of cholesteryl ester to total cholesterol, the distribution of phospholipids classes, triglyceride concentration and the fatty acid content were similar to normal values. However, the free glycerol content of the plasma was eight‐fold higher than normal. Erythrocyte lipids contain an elevated level of cholesterol and control levels of phospholipid content, phospholipid distribution and fatty acid content. The plasma was separated by ultracentrifugation into VLDL (density <1.006), LDL (1.006< d <1.063) and HDL (1.063< d< 1.21). Significant decreases in cholesterol and phospholipid were observed in LDL and HDL.

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“…Owing to zwitterionic polar heads and hydrophobic tails in phospholipid molecules and thus their inherent chemical nature, they are often co‐extracted with the compounds of interest during sample preparation and eluted at a wide range of retention times, resulting in ion suppression in ESI–MS (Chambers et al, ; Little et al, ). Phosphatidylcholines, sphingomyelins and lysophosphatidylcholines constitute the vast majority of total plasma phospholipids and cause most MEs in LC–MS‐based analytical methods for blood samples (Pang, Liang, Wang, Ping, & Luo, ; Schwarz, Dahlke, & Dreisbach, ). In our method, a sorbent‐containing filtration plate, Phree™ phospholipid‐removal plate (Phenomenex), was chosen to remove phospholipids as it has the ability to filter protein precipitate and selectively remove phospholipids.…”

Section: Resultsmentioning

confidence: 99%

“…constitute the vast majority of total plasma phospholipids and cause most MEs in LC-MS-based analytical methods for blood samples (Pang, Liang, Wang, Ping, & Luo, 2008;Schwarz, Dahlke, & Dreisbach, 1977). In our method, a sorbent-containing filtration plate, Phree™ phospholipid-removal plate (Phenomenex), was chosen to remove phospholipids as it has the ability to filter protein precipitate and selectively remove phospholipids.…”

Section: Phosphatidylcholines Sphingomyelins and Lysophosphatidylchomentioning

confidence: 99%

Simultaneous determination of amitraz, chlordimeform, formetanate and metabolites in human blood by liquid chromatography tandem mass spectrometry with phospholipid‐removal pretreatment

Gao

Sun

Hai-dan

et al. 2019

Biomedical Chromatography

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In this study, a reliable ultra‐performance liquid chromatography tandem mass spectrometry (UPLC–MS/MS) method coupled with an easy, fast and effective sample pretreatment procedure was developed for simultaneous determination of amitraz, chlordimeform, formetanate and their metabolites in human blood. With the procedures of protein precipitation and a phospholipid‐removal step, the endogenous compound interference was significantly reduced, and matrix effects were significantly reduced. The linear ranges of matrix‐matched standard curves were from 0.5 to 1000 ng/mL with coefficients of determination >0.996. Very low limits of detection (0.05–0.12 ng/mL) and limits of quantitation (0.15–0.4 ng/mL) were achieved. Reasonable recoveries ranging from 88.1 to 103.5% were obtained. The intra‐day RSDs ranging from 3.2 to 8.6% and inter‐day RSDs ranging from 4.8 to 9.2% indicated good precision. With the introduction of a phospholipid‐removal step, the ME ranged from 90.1 to 98.5%. The established method was successfully applied to the analysis of a blood sample from a formetanate poisoning case. This method possesses the advantages of high sensitivity, reduced matrix effects and rapidity.

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Phospholipid composition of blood plasma, erythrocytes, and "ghosts" in sickle cell disease.

Cited by 30 publications

References 0 publications

Phospholipids in liquid chromatography/mass spectrometry bioanalysis: comparison of three tandem mass spectrometric techniques for monitoring plasma phospholipids, the effect of mobile phase composition on phospholipids elution and the association of phospholipids with matrix effects

Phospholipids in liquid chromatography/mass spectrometry bioanalysis: comparison of three tandem mass spectrometric techniques for monitoring plasma phospholipids, the effect of mobile phase composition on phospholipids elution and the association of phospholipids with matrix effects

Plasma and erythrocyte lipids in sickle cell anaemia

Simultaneous determination of amitraz, chlordimeform, formetanate and metabolites in human blood by liquid chromatography tandem mass spectrometry with phospholipid‐removal pretreatment

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