Simultaneous determination of amitraz, chlordimeform, formetanate and metabolites in human blood by liquid chromatography tandem mass spectrometry with phospholipid‐removal pretreatment

Abstract

In this study, a reliable ultra‐performance liquid chromatography tandem mass spectrometry (UPLC–MS/MS) method coupled with an easy, fast and effective sample pretreatment procedure was developed for simultaneous determination of amitraz, chlordimeform, formetanate and their metabolites in human blood. With the procedures of protein precipitation and a phospholipid‐removal step, the endogenous compound interference was significantly reduced, and matrix effects were significantly reduced. The linear ranges of matrix‐matched standard curves were from 0.5 to 1000 ng/mL with coefficients of determination >0.996. Very low limits of detection (0.05–0.12 ng/mL) and limits of quantitation (0.15–0.4 ng/mL) were achieved. Reasonable recoveries ranging from 88.1 to 103.5% were obtained. The intra‐day RSDs ranging from 3.2 to 8.6% and inter‐day RSDs ranging from 4.8 to 9.2% indicated good precision. With the introduction of a phospholipid‐removal step, the ME ranged from 90.1 to 98.5%. The established method was successfully applied to the analysis of a blood sample from a formetanate poisoning case. This method possesses the advantages of high sensitivity, reduced matrix effects and rapidity.

Rapid and sensitive detection of chlordimeform in cucumber and tomato samples using an immunochromatographic assay

“…Several instrumental methods have been used to determine CDM, including liquid chromatography-tandem mass spectrometry (LC-MS/MS), 9 gas chromatography-mass spectrometry (GC-MS), 10 and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). 11 Even though these chromatographic methods offer accurate and effective information, they are time consuming, and require expensive instrumentation and complicated operation steps such as extraction, purification and derivatization.…”