Phosphinate–benzoindocyanin fluorescent probe for endogenous mitochondrial peroxynitrite detection in living cells and gallbladder access in inflammatory zebrafish animal models

Sonawane, Prasad M.; Lee, Won Chul; Kim, Yunsu; Roychaudhury, Arkaprava; Bhosale, Vikas K.; Kim, Donghyeon; Park, Hee Sung; Kim, Cheol-Hee; Churchill, David G.

doi:10.1016/j.saa.2021.120568

Cited by 15 publications

(6 citation statements)

References 53 publications

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“…The advantages of luminescence make these probes reliable for use in both in vivo and in vitro bioimaging. 13–17…”

Section: Introductionmentioning

confidence: 99%

“…The advantages of luminescence make these probes reliable for use in both in vivo and in vitro bioimaging. [13][14][15][16][17] Currently, a variety of fluorescent molecules have been determined for F − detection. [18][19][20][21] Some strategies deployed so far for the detection of F − are boron-fluoride complexation, 22 H-bonding probe 23 and Si-O/Si-C cleavage.…”

Section: Introductionmentioning

confidence: 99%

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“Lighting up” fluoride: cellular imaging and zebrafish model interrogations using a simple ESIPT-based mycophenolic acid precursor-based probe

Jain

Sonawane

Liu

et al. 2023

Analyst

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“…The advantages of luminescence make these probes reliable for use in both in vivo and in vitro bioimaging. 13–17…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“Lighting up” fluoride: cellular imaging and zebrafish model interrogations using a simple ESIPT-based mycophenolic acid precursor-based probe

Jain

Sonawane

Liu

et al. 2023

Analyst

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“…The dynamic levels of ONOO – are normally regulated by its redox homeostasis. Abnormal generation of ONOO – in organelles, for example, the mitochondrion, has been closely associated with inflammation and cancer. − Monitoring the levels of ONOO – and its redox homeostasis changes in organelles has emerged as a potential link between normal, inflammation, and progressing cancer. ,, …”

Section: Introductionmentioning

confidence: 99%

Dual-Channel Fluorescent Probe for the Detection of Peroxynitrite and Glutathione in Mitochondria: Accurate Discrimination of Inflammatory and Progressing Tumor Cells

et al. 2022

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Distinguishing between normal, inflammatory, and progressing tumor cells plays a vital role in early diagnoses and clinical studies. The simultaneous quantification of multiple biomarkers in cells can reveal cellular heterogeneity, which contributes to the discrimination of different types of cells. Herein, a dual-channel fluorescent probe has been developed for monitoring peroxynitrite (ONOO–) and glutathione (GSH) to accurately discriminate normal cells, inflammatory cells, and progressing cancer cells. The probe can monitor exogenous and endogenous mitochondrial GSH and ONOO– in living cells and zebrafish by green (530 nm, G530) and red (630 nm, R630) emission based on its good selectivity and low biotoxicity. GSH and ONOO– are visualized via fluorescence imaging, and the corresponding output signals can be employed to differentiate nontumorigenic, malignant, and metastatic breast cells in cocultured cells. Furthermore, the accurate discrimination among normal, inflammatory, and cancerous cells is achieved through the changes in the dual-channel fluorescence signal, which shows great potential for the diagnosis of inflammation and cancer diseases.

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“…Till date, several elaborate fluorescence probes have been engineered to monitor the mitochondrial ONOO – in live systems. − These mitochondria-targeted probes are rationally designed by equipping with lipophilic cations as the targeting group, which displays strong electrostatic attraction to the mitochondrial membrane maintaining a negative potential and thus guides the probes to accumulate into this organelle. However ONOO – in cytosol may react with the probes and induce fluorescence variation before they enter mitochondria, thus probably giving a false result on the mitochondrial ONOO – level and leading to the misjudgment on its biofunction.…”

mentioning

confidence: 99%

Photocontrollable Fluorescence Imaging of Mitochondrial Peroxynitrite during Ferroptosis with High Fidelity

Liu

Zhao

et al. 2022

Anal. Chem.

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Phosphinate–benzoindocyanin fluorescent probe for endogenous mitochondrial peroxynitrite detection in living cells and gallbladder access in inflammatory zebrafish animal models

Cited by 15 publications

References 53 publications

“Lighting up” fluoride: cellular imaging and zebrafish model interrogations using a simple ESIPT-based mycophenolic acid precursor-based probe

“Lighting up” fluoride: cellular imaging and zebrafish model interrogations using a simple ESIPT-based mycophenolic acid precursor-based probe

Dual-Channel Fluorescent Probe for the Detection of Peroxynitrite and Glutathione in Mitochondria: Accurate Discrimination of Inflammatory and Progressing Tumor Cells

Photocontrollable Fluorescence Imaging of Mitochondrial Peroxynitrite during Ferroptosis with High Fidelity

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