Phosphatidylglycerol Is Involved in the Dimerization of Photosystem II

Kruse, Olaf; Hankamer, Ben; Konczak, Carsten; Gerle, Christoph; Morris, Edward P.; Radunz, Alfons; Schmid, Georg; Barber, James

doi:10.1074/jbc.275.9.6509

Cited by 160 publications

(102 citation statements)

References 48 publications

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“…In addition to these 22 helices we have identi¢ed at least ten further transmembrane helices in the PS II core complex ; B. Hankamer, E. P. Morris and J. Barber, unpublished data). One of these helices belongs to PsbH and, although not yet con¢rmed, we believe this protein to be located at the interface between the two monomers within the dimer where it may play a role in stabilizing the dimeric con¢guration by interacting in its phosphorylated or non-phosphorylated state with the thylakoid lipid phosphatidylglycerol which has the usual transhexadecanoic fatty acid (Kruse et al 2000).…”

Section: Discussionmentioning

confidence: 99%

Supermolecular structure of photosystem II and location of the PsbS protein

Nield

Funk

Barber

2000

Phil. Trans. R. Soc. Lond. B

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This paper addresses the question of whether the PsbS protein of photosystem two (PS II) is located within the LHC II^PS II supercomplex for which a three-dimensional structure has been obtained by cryoelectron microscopy and single particle analysis. The PsbS protein has recently been implicated as the site for non-photochemical quenching. Based both on immunoblotting analyses and structural considerations of an improved model of the spinach LHC II^PS II supercomplex, we conclude that the PsbS protein is not located within the supercomplex. Analyses of other fractions resulting from the solubilization of PS II-enriched membranes derived from spinach suggest that the PsbS protein is located in the LHC II-rich regions that interconnect the supercomplex within the membrane.

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Section: Discussionmentioning

confidence: 99%

Supermolecular structure of photosystem II and location of the PsbS protein

Nield

Funk

Barber

2000

Phil. Trans. R. Soc. Lond. B

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“…PG plays an important role in the oligomerization of the light harvesting complex and the dimerization of PSII (McCourt et al, 1985;Krupa et al, 1987;Kruse et al, 2000). It is also required for both the electron-acceptor side and the donor side of PSII (Sakurai et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Impact of UV-B on drought- or cadmium-induced changes in the fatty acid composition of membrane lipid fractions in wheat

Gondor

Szalai

Kovács

et al. 2014

Ecotoxicology and Environmental Safety

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Several reports have demonstrated the direct and indirect effects of UV-B radiation, an integral component of sunlight, on the plant growth and metabolism of various plant species, including a range of morphological, physiological and biochemical changes (Zlatev et al., 2012). However, as well as being a damaging agent UV-B also has an important role as a regulatory signal. The perception of and responses to UV-B by plants stimulate protective mechanisms, including the accumulation of UV-absorbing phenolic compounds and the modification of the biochemical composition (Jenkins, 2008). Under natural conditions plants are often subjected to multiple stress factors, so the impact of any particular stress may be aggravated or attenuated by the simultaneous action of another stressor. In a previous study it was demonstrated that UV-B radiation may have either a positive or negative impact under the same conditions in wheat, depending on the type of secondary abiotic stress factor: Cd or drought (Kovács et al., 2014). Supplemental UV-B light prevented the wilting and leaf rolling induced by PEG treatment. In contrast, combined UV-B and Cd treatment resulted in pronounced oxidative stress, which was manifested in the further enhancement of the leaf MDA content, root antioxidant enzyme activities and also root polyamine content compared to the effect of single stress factors, Cd or UV-B radiation (Kovács et al., 2014).The ability to adjust membrane lipid fluidity by changing the level of unsaturated fatty acids is a feature of stress-tolerant plants, resulting in an environment suitable for the function of critical integral proteins. Changes have been described in the total lipid content, in the saturation of the total lipids, in the ratio of the membrane lipid fractions, and in the fatty acid composition of individual membrane fractions during single abiotic stresses, such as drought (Zhong et al., 2011;Filek et al., 2012) or heavy metal (Pál et al., 2007;Bernat et al., 2014). Nevertheless, no reports have yet been published on the combined effect of either drought or Cd treatment and UV-B radiation on the fatty acid composition of the individual membrane fractions in wheat plants. Impact of UV-B on drought-or cadmium-induced changes in the fatty acid composition of membrane lipid fractions in wheat AbstractUV-B radiation may have either a positive or negative impact under the same conditions in wheat, depending on the type of secondary abiotic stressor: Cd or drought. Supplemental UV-B prevented the wilting and leaf rolling induced by PEG treatment. In contrast, combined UV-B and Cd treatment resulted in pronounced oxidative stress. The opposite effect of UV-B radiation in the case of drought or cadmium stress may be related to the alteration induced in the fatty acid composition. UV-B caused changes in the unsaturation of leaf phosphatidylglycerol fractions, and the accumulation of flavonoid in the leaves may prevent the stress induced by subsequent drought treatment. However it resulted in pronounced injury despite ...

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“…Particularly one protein in this complex called intrinsic D1 core peptide has high binding affinity for PG which promotes dimerization of the complex. Thin layer chromatography analysis showed that photosystem II dimers contained four times more PG than their monomeric counterparts but with similar levels of phosphatidylcholine (49). PG depletion from thylakoid membrane in PG mutants causes dissociation of extrinsic proteins and PSII dysfunction (50).…”

Section: Pg In Photosynthesis and Electron Chain Transportmentioning

confidence: 99%