Phorbol ester/calcium ionophore activate fish leukocytes and induce long-term cultures

Lin, Guilan; Ellsaesser, Catherine F.; Clem, L. William; Miller, Norman W.

doi:10.1016/0145-305x(92)90015-5

Cited by 61 publications

(22 citation statements)

References 25 publications

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“…During the past decade, much research has focused on teleostean immunology and immunopathology and has shown that bony fish possess cellular repertoires and immunological activities similar to those present in higher vertebrates. Teleost leukocyte populations can be di#erentiated into granulocytes (Ainsworth, 1992;Hine, 1992), macrophages (Meseguer et al, 1991;Hardie et al, 1994;Sveinbjornsson & Seljelid, 1994), thrombocytes (Esteban et al, 1989), B lymphocytes (Irwin & Kaattari, 1986;Miller et al, 1994;Koumans-Van Diepen et al, 1994a) and T lymphocytes (Lin et al, 1992;Miller et al, 1994).…”

Section: Introductionmentioning

confidence: 96%

Production and characterisation of a monoclonal antibody against the thymocytes of the sea bassDicentrarchus labrax(L.) (Teleostea, Percicthydae)

Scapigliati

Mazzini

Mastrolia

et al. 1995

Fish & Shellfish Immunology

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Section: Introductionmentioning

confidence: 96%

Production and characterisation of a monoclonal antibody against the thymocytes of the sea bassDicentrarchus labrax(L.) (Teleostea, Percicthydae)

Scapigliati

Mazzini

Mastrolia

et al. 1995

Fish & Shellfish Immunology

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“…130 Further evidence for the presence of 5-HT 3 receptors in trout lymphocytes was provided by Ferrière and colleagues. 131 As increases in intracellular Ca 2+ levels have been associated with an induction of leukocyte proliferation in catfish, 132 the main objective of their experiments was to examine the effects of 5-HT on calcium signaling in peripheral blood lymphocytes of rainbow trout. Isolated trout lymphocytes were exposed to 25 µM and 50 µM 5-HT and cytosolic Ca 2+ concentrations were calculated based on fluorescence measurements.…”

Section: -Ht and The Immune Systemmentioning

confidence: 99%

Physiological Endpoints for Potential SSRI Interactions in Fish

Kreke

Dietrich

2008

Critical Reviews in Toxicology

114

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Selective serotonin reuptake inhibitors (SSRIs) are among the pharmaceutical compounds frequently detected in sewage treatment plant effluents and surface waters, albeit at very low concentrations, and have therefore become a focus of interest as environmental pollutants. These neuroactive drugs are primarily used in the treatment of depression but have also found broader use as medication for other neurological dysfunctions, consequently resulting in a steady increase of prescriptions worldwide. SSRIs, via inhibition of the serotonin (5-hydroxytryptamine, 5-HT) reuptake mechanism, induce an increase in extracellular 5-HT concentration within the central nervous system of mammals. The phylogenetically ancient and highly conserved neurotransmitter and neurohormone 5-HT has been found in invertebrates and vertebrates, although its specific physiological role and mode of action is unknown for many species. Consequently, it is difficult to assess the impact of chronic SSRI exposure in the environment, especially in the aquatic ecosystem. In view of this, the current knowledge of the functions of 5-HT in fish physiology is reviewed and, via comparison to the physiological role and function of 5-HT in mammals, a characterization of the potential impact of chronic SSRI exposure on fish is provided. Moreover, the insight on the physiological function of 5-HT strongly suggests that the experimental approaches currently used are inadequate if not entirely improper for routine environmental risk assessment of pharmaceuticals (e.g., SSRIs), as relevant endpoints are not assessed or impossible to determine.

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“…It is possible that the activity induced by UV-CRV in fibroblasts is type I interferon (interferon &P), whereas the activity seen in T cells and macrophages is related to type I1 interferon (interferon y). Cytokine activity has been detected in catfish lymphoid cell cultures following mitogen stimulation (Caspi & Avtalion 1984, Grondel & Harmsen 1984, Lin et al 1992, Clem et al 1996. Furthermore, rapidlyproliferating MLC cultures contain increased numbers of activated natural killer and/or T cells (Stuge et al 1997) and likely synthesize interferon y and a variety of other cytokines.…”

Section: Discussionmentioning

confidence: 99%

Channel catfish reovirus (CRV) inhibits replication of channel catfish herpesvirus (CCV) by two distinct mechanisms:viral interference and induction of an anti-viral factor

Chinchar

Logue²,

Antao³

et al. 1998

Dis. Aquat. Org.

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Catfish reovirus (CRV), a double-stranded RNA virus, inhibited channel catfish herpesvirus (CCV) replication by 2 different mechanisms: (1) directly as a consequence of its own replication, and (2) indirectly due to the induction of an anti-viral factor. In the former, prior infection with CRV significantly reduced subsequent CCV protein synthesis and virus yield. CRV-mediated interference was greatest when CRV infection preceded CCV infection by 16 h, and was least when cell cultures were simultaneoulsy infected with both viruses. In the latter case, infection of channel catfish ovary (CCO) cultures with UV-inactivated CRV resulted in the synthesis (or release) of an anti-viral factor. Cells producing the factor were protected from CCV infection, as were cells which had been treated with spent culture medium containing anti-viral activity. Interestingly an anti-viral activity was constitutively present in long-term cultures of catfish T cells and macrophages. Whether this factor and the one induced by UV-inactivated CRV are identical is not known, but analogy to mammalian systems suggests that the former may be similar to type I1 interferon, whereas the latter may be the piscine equivalent of type I interferon. These results suggest that UV-inactivated CRV may prove useful in the induction and characterization of interferon-like anti-viral proteins in the channel catfish and that long-term cultures of catfish T cells and monocytes may serve as a ready source of additional anti-viral factors.

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Phorbol ester/calcium ionophore activate fish leukocytes and induce long-term cultures

Cited by 61 publications

References 25 publications

Production and characterisation of a monoclonal antibody against the thymocytes of the sea bassDicentrarchus labrax(L.) (Teleostea, Percicthydae)

Production and characterisation of a monoclonal antibody against the thymocytes of the sea bassDicentrarchus labrax(L.) (Teleostea, Percicthydae)

Physiological Endpoints for Potential SSRI Interactions in Fish

Channel catfish reovirus (CRV) inhibits replication of channel catfish herpesvirus (CCV) by two distinct mechanisms:viral interference and induction of an anti-viral factor

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