Polycystic ovary syndrome (PCOS) is an endocrine disease that affects the health of many women. Circular RNAs (circRNAs) are associated with the occurrence and progression of PCOS. This study aimed to explore the function of circ_RANBP9 in PCOS. First, the circ_RANBP9 level was found to be increased in the plasma of patients with PCOS and ovarian granulosa cells (GCs) using Reverse Transcription-Quantitative Polymerase Chain Reaction (RT-qPCR). In GCs, loss of circ_RANBP9 decelerated proliferation and accelerated apoptosis of KGN and COV434 cells, as determined by MTT assay, colony formation assay, and flow cytometry. Furthermore, bioinformatics analysis showed that circ_RANBP9 and XIAP can be targeted by the microRNA, miR-136-5p. Luciferase reporter assay and RNA pull-down assay further verified the interaction between miR-136-5p and circ_RANBP9 or XIAP. Importantly, knockdown of circ_RANBP9 suppressed proliferation and promoted apoptosis of KGN and COV434 cells, whereas inhibition of miR-136-5p reversed these effects. Additionally, XIAP abolished the repression of proliferation and acceleration of apoptosis induced by miR-136-5p. The promotion of apoptosis was accompanied by upregulation of caspase-3 and Bax, and downregulation of Bcl-2, as estimated by western blotting. In conclusion, silencing of circ_RANBP9 inhibited GC proliferation and facilitated apoptosis by mediating the miR-136-5p/XIAP pathway. These findings provide a new theoretical basis for screening and treatment of PCOS.
The decreasing of H3K9Ac and H4K20TriMe is possibly related with the occurrence of epithelial ovarian tumors. The more significant the expression of H3K9Ac and H4K20TriMe was, the lower the histological grading and the later the clinical staging were. H3K9Ac and H4K20TriMe were potential biomarkers for prognosis. H3K14Ac showed no significant relation with the occurrence and development of ovarian tumors.
I(K,ADO) contributes significantly to the negative dromotropic effect of adenosine, but predominantly at relatively high concentrations of the nucleoside.
Several studies have assessed the association of CD95L polymorphism with cervical cancer risk, but the data lack the power to provide compelling evidence. In this study, we aimed to clarify the association through a meta-analysis. A comprehensive search was conducted in PubMed, Embase, and Web of Science. The fixed-effects model was used to calculate odds ratio (OR) with 95 % confidence intervals (CIs). A total of five papers with six case-control studies were derived and finally included in this meta-analysis. The overall estimate did not reveal any significant association between CD95L -844C/T polymorphism and cervical cancer risk. Subgroup analysis in Asian population indicated nonsignificant nevertheless potentially increased risk in CC genotype carriers in comparison with the carriers of CT+TT genotypes (ORCC vs. CT+TT=1.16, 95 % CI=0.99-1.36, P for heterogeneity=0.231). Based on current epidemiological studies, this meta-analysis suggests that CD95L polymorphism may not be a risk factor contributing to cervical cancer development.
OBJECTIVE: T o determine b y measuring sitting and standing height, whether growth failure in homozygous p-thalassemia is disproportionate. METHODS:Management of all patients with homozygous j3-thalassemia in the State of Victoria (Australia) is centralised t o o n e major teaching hospital; therefore treatment protocols are relatively standardised, a n d this group represents a population based cohort. Patients are transfused every 3-4 weeks to maintain hemoglobin values >10 g%, and desfemioxamine 6 0 m g ' k d d , to a maximum of 3glkay, is given S.C. for iron chelation. Measurements were made using Iloltain sitting and Harpendon standing stadiometers, in a random sample of 52 of 122 (43%) patients t o date. Subischid leg length was determined by subtraction of sitting height from standing height. Standard deviation scores (sds) were used to enable comparisons irrespective of chronological age, and the patient group was analysed according t o age: group 1 -<18 y.o., a n d group 2 -2 1 8 y.0. A l l GH pulses coincident with Zn There was no s i g n i f i c a n t correlation between individual sums of GH & Zn (r2=0.14). These data aupport t h e hypothesis t h a t t h e release of endogenous Growth Hormone i s associated with a release of Zinc.
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INSULIN-LIKE GROWTH FACTOR BINDING PROTEINS FROM PRIMARY CULTURES OF RAT OVARIAN TIIECA-INTERSTITIAL (ROTI) CELLS:REGULATION BY LEI AND IGF-I. J. F. Cara and J. Fan, Dept. of Peds., University of Chicago Pritzker School of Medicine, Chicago, IL 60637. USAWe and others have shown that insulin-like growth factor-I (IGF-I) and IGF binding proteins (IGFBPs) play an important role in ovarian function. In the present study, we tested the hypothesis that ROTI cells produce IGFBPs and that theca-interstitial cell IGFBP production is regulated by LH and IGF-I.Density gradient-purified ROT1 cells were cultured in serum-free medium with selected concentrations of LII or IGF-I, alone and in combination. In uremia reduced longitudinal growth and decreased hepatic IGF-I secretion despite elevated GH serum levels point to a resistance to the action of GH, which could be a consequence of a reduced hepatic GI1 receptor (R) expression. To addressthis hypothesis we studied the hepatic GH-R mRNA content in uremic female SD rats (n=7 per group) subjected to 516 nephrectomy (U) compared to sham operated pairfed (PC) or ad libitum fed (aLC) controls. Animals were treated with 10 IU rhGHlkglday, or solvent for 10 days. Total RNA was prepared from rapidly frozen liver tissue and the GH-R mRNA quantified by solution hybridization. Mean (kSD) weight gain was lower in U (21.4f7.9 g) compared to PC (24.1f5.9; P<0.005) and aLC (33.5f6.3; P<0.005). Hepatic GH-R mRNA was reduced in U (0.79f0.39 amollug DNA) vs PC (1.46f0.32; P<0.005) and vs aLC (2.66-10.73, P
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