Phenotypic Features of BHK-21 Cells Used for Production of Foot-and-mouth Disease Vaccine

Amadori, Massimo; Volpe, Gisella; Defilippi, Paola; Berneri, C.

doi:10.1006/biol.1996.0061

Cited by 21 publications

(14 citation statements)

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“…The characteristics, species and tissues of origin, growth media, and sources of the cell lines used in this study are listed in Table 1. SA-dependent simian SA11 Cl3 (P5B [2], G3) and RRV (P5B [3], G3), equine H-1 (P9 [7], G5), bovine NCDV (P6 [1], G6), and porcine OSU (P9 [7], G5), SA-independent human Wa (P1A [8], G1), DS-1 (P1B [4], G2), HAL1166 (P11 [14], G8), and PA169 (P11 [14], G6), equine H-2 (P4 [12], G3), lapine ALA (P11 [14], G3), BAP-2 (P11 [14], G3), and C-11 (P11 [14], G3), and bovine WC3 (P7 [5], G6) rotavirus strains and the SA-independent SA11Cl3 rotavirus variant 954/23R (SA11 Cl3 954/23R) strain were cultivated in rhesus monkey kidney cells (MA104) in the presence of trypsin as described previously (12).…”

Section: Methodsmentioning

confidence: 99%

“…The ␣2 integrin subunit was not detected in guinea pig GPC-16 cells when antibody to the human ␣2 subunit of VLA-2 was used, but it was detected in small amounts when antibody to the mouse or rat ␣2 subunit of VLA-2 was used (data not shown). Since an antibody to the guinea pig ␣2 subunit of VLA-2 is not available, it is possible that the limited detection of guinea pig VLA-2 is due to not having the proper species-specific antibody to detect it, because the ␣2 subunit of VLA-2, unlike the ␤1 subunit, is not highly conserved across species (1,22). Except for CHO-K1 and CHO-p901, all cells were shown to express the ␤1 integrin subunit (Fig.…”

Section: Cellular Expression Of Vla-2 Integrin In Cell Lines Exhibitimentioning

confidence: 99%

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VLA-2 (α2β1) Integrin Promotes Rotavirus Entry into Cells but Is Not Necessary for Rotavirus Attachment

Ciarlet

Crawford

Cheng

et al. 2002

J Virol

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In an attempt to identify the rotavirus receptor, we tested 46 cell lines of different species and tissue origins for susceptibility to infection by three N-acetyl-neuraminic (sialic) acid (SA)-dependent and five SA-independent rotavirus strains. Susceptibility to SA-dependent or SA-independent rotavirus infection varied depending on the cell line tested and the multiplicity of infection (MOI) used. Cells of renal or intestinal origin and transformed cell lines derived from breast, stomach, bone, or lung were all susceptible to rotavirus infection, indicating a wider host tissue range than previously appreciated. Chinese hamster ovary (CHO), baby hamster kidney (BHK-21), guinea pig colon (GPC-16), rat small intestine (Rie1), and mouse duodenum (MODE-K) cells were found to support only limited rotavirus replication even at MOIs of 100 or 500, but delivery of rotavirus particles into the cytoplasm by lipofection resulted in efficient rotavirus replication. The rotavirus cell attachment protein, the outer capsid spike protein VP4, contains the sequence GDE(A) recognized by the VLA-2 (␣2␤1) integrin, and to test if VLA-2 is involved in rotavirus attachment and entry, we measured infection in CHO cells that lack VLA-2 and CHO cells transfected with the human ␣2 subunit (CHO␣2) or with both the human ␣2 and ␤1 subunits (CHO␣2␤1) of VLA-2. Infection by SA-dependent or SA-independent rotavirus strains was 2-to 10-fold more productive in VLA-2-expressing CHO cells than in parental CHO cells, and the increased susceptibility to infection was blocked with anti-VLA-2 antibody. However, the levels of binding of rotavirus to CHO, CHO␣2, and CHO␣2␤1 cells were equivalent and were not increased over binding to susceptible monkey kidney (MA104) cells or human colonic adenocarcinoma (Caco-2, HT-29, and T-84) cells, and binding was not blocked by antibody to the human ␣2 subunit. Although the VLA-2 integrin promotes rotavirus infection in CHO cells, it is clear that the VLA-2 integrin alone is not responsible for rotavirus cell attachment and entry. Therefore, VLA-2 is not involved in the initial attachment of rotavirus to cells but may play a role at a postattachment level.

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Section: Methodsmentioning

confidence: 99%

Section: Cellular Expression Of Vla-2 Integrin In Cell Lines Exhibitimentioning

confidence: 99%

VLA-2 (α2β1) Integrin Promotes Rotavirus Entry into Cells but Is Not Necessary for Rotavirus Attachment

Ciarlet

Crawford

Cheng

et al. 2002

J Virol

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“…However, contrary to the case in murine cells, a reduction in the antiviral properties of Ad5-poIRF7/3(5D) in hamster cells was not observed. In fact, it was surprising to detect the strong antiviral activity induced by Ad5-poIRF7/3(5D) in cell lines that have previously been reported to be defective in IFN-␣/␤ sensing or sig- naling (63,64) and that are routinely used for viral expansion and production of FMD vaccines (65). BHK-21 cells did not respond to poly(I·C) stimulation (or viral infection) but responded to treatment with the poIRF7/3(5D) protein, suggesting that expression of this protein bypasses certain limitations in antiviral pathways.…”

Section: Discussionmentioning

confidence: 99%

Expression of Porcine Fusion Protein IRF7/3(5D) Efficiently Controls Foot-and-Mouth Disease Virus Replication

Ramírez-Carvajal

Segundo

Hickman

et al. 2014

J Virol

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Several studies have demonstrated that the delivery of type I, II, or III interferons (IFNs) by inoculation of a replication-defective human adenovirus 5 (Ad5) vector expressing IFNs can effectively control foot-and-mouth disease (FMD) in cattle and swine during experimental infections. However, relatively high doses are required to achieve protection. In this study, we identified the functional properties of a porcine fusion protein, poIRF7/3(5D), as a biotherapeutic and enhancer of IFN activity against FMD virus (FMDV). We showed that poIRF7/3 ( IMPORTANCEFMD remains one of the most devastating diseases that affect livestock worldwide. Effective vaccine formulations are available but are serotype specific and require approximately 7 days before they are able to elicit protective immunity. We have shown that vector-delivered IFN is an option to protect animals against many FMDV serotypes as soon as 24 h and for about 4 days postadministration. Here we demonstrate that delivery of a constitutively active transcription factor that induces the production of endogenous IFNs and potentially other antiviral genes is a viable strategy to protect against FMD.

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“…BHK-21 is a hamster derived cell line and commonly used for FMD vaccine production [9]. The endogenous proteins produced and secreted by BHK-21 cells, as a kind of heterologous proteins to immune recipients, and induce immune response but non-related to vaccine per se.…”

Section: Bhk-21 Production Cellmentioning

confidence: 99%

Adverse Effects of Inactivated Foot-and-Mouth Disease Vaccine—Possible Causes Analysis and Countermeasures

Zhang¹,

Lu²,

Liu³

et al. 2018

WJV

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Foot-and-mouth disease (FMD) is an infectious and sometimes fatal viral disease that affects cloven-hoofed animals, and Chinese government adopts compulsory immunization measures for FMD. The adverse effects of FMD vaccine to pigs, cattle and goats have been reported increasingly frequent during the spring and autumn seasons when large numbers of farm livestock are vaccinated. The financial losses caused by vaccine adverse effects have been a serious concern for both farmers and primary prevention personnel. There are various causative factors reported to involve into adverse effect of FMD vaccine, including the inappropriate vaccine production, transportation and storage, livestock poor tolerance, and unqualified vaccinating manipulations. Symptomatic treatment and early drug prevention have a certain effect on the adverse effects. To analyze causes and propose countermeasures, in the current study possible reasons during the production and processing procedures of inactivated FMD vaccine were reviewed and corresponding countermeasures were recommended. The review may provide references for better use of vaccine to prevent FMD.

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Phenotypic Features of BHK-21 Cells Used for Production of Foot-and-mouth Disease Vaccine

Cited by 21 publications

References 0 publications

VLA-2 (α2β1) Integrin Promotes Rotavirus Entry into Cells but Is Not Necessary for Rotavirus Attachment

VLA-2 (α2β1) Integrin Promotes Rotavirus Entry into Cells but Is Not Necessary for Rotavirus Attachment

Expression of Porcine Fusion Protein IRF7/3(5D) Efficiently Controls Foot-and-Mouth Disease Virus Replication

Adverse Effects of Inactivated Foot-and-Mouth Disease Vaccine—Possible Causes Analysis and Countermeasures

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