Introduction: Orf virus (ORFV) is a prototype Parapoxvirus species in the Poxviridae family that causes serious zoonotic infectious disease. Goat skin fibroblast (GSF) cells are the major host targets of ORFV. Interleukin 8 (IL-8) and tumour necrosis factor (TNF)-α are known to play a vital role in immune response during viral infections. However, the manner of variation over time of their level of expression in GSF cells remains unclear. Material and Methods: In this study, quantitative enzyme-linked immunosorbent assay chips were used to detect changes in the levels of these cytokines expressed and secreted in GSF cells after ORFV infection. Results: Results showed that the expression of IL-8, TNF-α, and decorin was upregulated in the cell lysates, and that secreted decorin and IL-8 were significantly increased in cell supernatant.
Conclusion:The results provided possible approaches to elucidation of how ORFV infection initiates host cell immune response.
The clinical symptoms and lesions caused by SVA are indistinguishable from those of other vesicular diseases in pigs. SVA has been associated with recent outbreaks of vesicular disease and epidemic transient neonatal losses in several swine-producing countries.
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