Phenotypic and molecular characterization of Colletotrichum species associated with anthracnose of banana (Musa spp) in Malaysia

Abstract

ABSTRACT. Anthracnose caused by Colletotrichum species is a common postharvest disease of banana fruit. We investigated and identified Colletotrichum species associated with anthracnose in several local banana cultivars based on morphological characteristics and sequencing of ITS regions and of the β-tubulin gene. Thirty-eight Colletotrichum isolates were encountered in anthracnose lesions of five local banana cultivars, 'berangan', 'mas', 'awak', 'rastali', and 'nangka'. Based on morphological characteristics, 32 isolates were identified as Colletotrichum gloeosporioides and 6 isolates as C. musae. C. gloeosporioides isolates were divided into two morphotypes, with differences in colony color, shape of the conidia and growth rate. Based on ITS regions and β-tubulin sequences, 35 of the isolates were identified as C. gloeosporioides and only 3 isolates as C. musae; the percentage of similarity from BLAST ranged from 95-100% for ITS regions and 97-100% for β-tubulin. C. gloeosporioides isolates were more prevalent compared to C. musae. This is the first record of C. gloeosporioides associated with banana anthracnose in Malaysia. In a phylogenetic analysis of the combined dataset of ITS regions and β-tubulin using a maximum likelihood method, C. gloeosporioides and C. musae isolates were clearly separated into two groups. We concluded that C. gloeosporioides and C. musae isolates are associated with anthracnose in the local banana cultivars and that C. gloeosporioides is more prevalent than C. musae.

Keberadaan Mikovirus Berdasarkan Deteksi Berbasis RNA pada Colletotrichum Hipovirulen

“…Colletotrichum merupakan salah satu kelompok patogen tanaman penting yang menyebabkan kerugian ekonomi yang besar di daerah tropis dan subtropis (Sakinah et al 2014). Colletotrichum menyebabkan penyakit antraknosa pada lebih dari 3200 spesies monokotil dan dikotil.…”

Role of phospholipase C in banana in response to anthracnose infection

“…However, because of their highly perishable nature, banana fruits usually have a short shelf life after harvest under tropical conditions (Luo et al; Wang et al). Banana anthracnose, also known as black rot, is a fungal disease caused by Calletotrichum musae that widely occurs in all banana areas, mainly causing fruit damage (Intan Sakinah, Suzianti, & Latiffah, ), which is a common postharvest disease of banana fruits (Abayasekara, Adikaram, Wanigasekara, & Bandara, ). Postharvest disease significantly reduces the commercial value of banana fruits (Campuzano, Rosell, & Cornejo, ; Wang et al, ).…”

“…Postharvest disease significantly reduces the commercial value of banana fruits (Campuzano, Rosell, & Cornejo, 2018;Wang et al, 2013). To date, the control of banana anthracnose mainly includes resistant variety selection; clearing and destroying the source of the disease in time; and early bagging, chemical control, and fruit harvest on sunny days (Intan Sakinah et al, 2014;Sridhar & Sohi, 1974). However, the incidence of this disease remains high.…”

Morphological and molecular identification of Colletotrichum species associated with cassava anthracnose in Thailand

“…Molecular techniques such as polymerase chain reaction (PCR) and sequencing of internal transcribed spacer (ITS) regions can be used to resolve this problem. Within a species complex (Du, Schardl, Nuckles, & Vaillancourt, ; Phoulivong et al., ; Sakinah, Suzianti, & Latiffah, ), direct analysis of DNA sequences will help resolve relationships among the species (Du et al., ). Although CAD was found in Thailand several years ago, identification of the causal agent has not yet been determined.…”

“…Within a species complex (Du, Schardl, Nuckles, & Vaillancourt, 2005;Phoulivong et al, 2010;Sakinah, Suzianti, & Latiffah, 2014), direct analysis of DNA sequences will help resolve relationships among the species (Du et al, 2005). Although CAD was found in Thailand several years ago, identification of the causal agent has not yet been determined.…”