Keberadaan Mikovirus Berdasarkan Deteksi Berbasis RNA pada Colletotrichum Hipovirulen

Abstract

Colletotrichum is one of phytopathogenic fungi causing large economic losses in more than 3000 plant species in the world. Colletotrichum causes anthracnose disease with sunken and concentric ring-shaped of necrosis symptoms on leaves and fruits. Chilli production has reported to have yield losses 10%-80% and production of Acacia spp. experiencing losses at the nursery stage due to anthracnose. Mycovirus is a biological control agent that has potential to develop through its hypovirulence effect generated on host pathogenic fungi. Mycovirus infect host fungi and replicate rapidly in host cells also can be transmitted through horizontal and vertical transmission. This research was purposed to detect the existence of mycoviruses in hypovirulent Colletotrichum. Stages of this research comprised Colletotrichum isolation, colony morphological observation, virulence assay on apples fruit, and RNA analysis included RNA extraction based on PrestoTM Mini RNA Yeast Kit protocol and RNA visualization on agarose gel using UV transilluminator. The results of RNA visualization showed the existence of specific RNA bands outside rRNA and tRNA bands in D3 and D9 isolates which were considered as RNA bands of mycovirus. The specific RNA bands in D3 isolate sized around 2 kbp and 3 kbp while D9 isolate showed specific RNA bands sized around 0.6 kbp and 3 kbp.