Phenotypic and genetic characterization of multidrug-resistant Salmonella Infantis strains isolated from broiler chicken meats in Turkey

Abstract

Twenty Salmonella Infantis strains resistant against kanamycin, tetracycline, neomycin, spectinomycin, sulphonamide, nalidixic acid and trimethoprim were selected for this study out of 103 Salmonella strains isolated from broiler samples collected from several markets in the Bolu and Ankara regions of Turkey. The resistance genes aadA1, aphA1, sul1, tet(A), dfrA5/dfrA14 and gyrA were determined for these multidrug-resistant S. Infantis strains. S. Infantis strains contained a mega plasmid with the molecular size of 206 kb. The strains were divided into three groups according to the pulsed field gel electrophoresis patterns of XbaI digested chromosomal DNA. A Ser83→Tyr83 point mutation was found in the gyrA gene of all quinolone-resistant isolates. Filter mating experiments showed that 206 kb plasmid transferred nalidixic acid resistance associated with class I integrons.

Prevalence of β-lactam and fluoroquinolone resistance, and virulence factors in Escherichia coli isolated from chickens in Slovakia

“…The high number of isolates resistant to tetracycline, streptomycin and sulphonamides can also be explained by the genetic linkage of antimicrobialresistance genes coding for these types of resistance and their location on the same mobile genetic elements (Dolejská et al 2007). A high number of isolates from chicken broilers carrying an aadA and dfr genes was also confirmed by Šeputiene et al (2010) and Abbasoglu & Akcelik (2011).…”

Genetic analysis of multiple antimicrobial resistance in Salmonella isolated from diseased broilers in Egypt

“…Class 1 integrons have been identified previously in S. enterica serovar Typhimurium isolated from diseased turkeys in the USA (15) and from animals in Italy (11), the USA (14) and Japan (8). Furthermore, class 1 integrons have previously been identified in many Salmonella serovars isolated from retail chicken meats in Japan (31) and more recently in China and Turkey (32,33). Of note, S. enterica serovar Typhimurium strain S12-2 displayed the multidrug resistant phenotype of SGI1, that is, resistant to AMP, CHL, STR, sulfonamides and TET (21) ( Table 3).…”

“…enterica serovar Typhimurium isolated from diseased turkeys in the USA (15) and from animals in Italy (11), the USA (14) and Japan (8). Furthermore, class 1 integrons have previously been identified in many Salmonella serovars isolated from retail chicken meats in Japan (31) and more recently in China and Turkey (32, 33). Of note, S .…”

“…Class 2 integrons have an organization similar to that of class 1 but are associated with transposon Tn 7 (33). They are also known to carry three classic gene cassettes; dfrA1 , sat2 and aadA1 , which confer resistance to trimethoprim, streptothricin and STR/SPX, respectively (35).…”

“…SGI1 has been found to be responsible for a characteristic multidrug resistance pattern associated with an epidemic strain of Salmonella Typhimurium DT104 (34). Class 2 integrons have an organization similar to that of class 1 but are associated with transposon Tn7 (33). They are also known to carry three classic gene cassettes; dfrA1, sat2 and aadA1, which confer resistance to trimethoprim, streptothricin and STR/SPX, respectively (35).…”