Phenolic compounds affect production of pyocyanin, swarming motility and biofilm formation of Pseudomonas aeruginosa

Uğurlu, Aylin Kılınç; Yağcı, Ayşegül; Ulusoy, Seyhan; Aksu, Burak; Tinaz, Gulgun Bosgelmez

doi:10.1016/j.apjtb.2016.06.008

Cited by 60 publications

(46 citation statements)

References 24 publications

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“…The compound ( Z , Z )‐5‐(trideca‐4′,7′‐dienyl)‐resorcinol that was isolated from the plant Lithrea molleoides significantly inhibited swarming motility of P. mirabilis at 28 μ m and completely abolished swarming at 433 μ m . Furthermore, many similar plant‐derived phenolic compounds including caffeic acid, cinnamic acid, ferulic acid, and vanillic acid have been reported to inhibit swarming of P. aeruginosa at 4 m m …”

Section: Secondary Plant Metabolitesmentioning

confidence: 99%

“…[98] Furthermore, many similarp lantderived phenolic compounds including caffeica cid, cinnamic acid, ferulica cid, and vanillic acid have been reported to inhibit swarming of P. aeruginosa at 4mm. [99] Also, tanninss uch as proanthocyanidins are important phenolic compounds produced by many plant species. Cranberry proanthocyanidin extracts and pomegranate extractsc ontaining the related punicalagin completely abolished swarming of P. aeruginosa at 100 mgmL À1 without inhibiting growth.…”

Section: Secondary Plant Metabolitesmentioning

confidence: 99%

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Inhibitors of Bacterial Swarming Behavior

Rütschlin

Böttcher

2019

Chemistry A European J

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Bacteria can migrate in groups of flagella‐driven cells over semisolid surfaces. This coordinated form of motility is called swarming behavior. Swarming is associated with enhanced virulence and antibiotic resistance of various human pathogens and may be considered as favorable adaptation to the diverse challenges that microbes face in rapidly changing environments. Consequently, the differentiation of motile swarmer cells is tightly regulated and involves multi‐layered signaling networks. Controlling swarming behavior is of major interest for the development of novel anti‐infective strategies. In addition, compounds that block swarming represent important tools for more detailed insights into the molecular mechanisms of the coordination of bacterial population behavior. Over the past decades, there has been major progress in the discovery of small‐molecule modulators and mechanisms that allow selective inhibition of swarming behavior. Herein, an overview of the achievements in the field and future directions and challenges will be presented.

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Section: Secondary Plant Metabolitesmentioning

confidence: 99%

Section: Secondary Plant Metabolitesmentioning

confidence: 99%

Inhibitors of Bacterial Swarming Behavior

Rütschlin

Böttcher

2019

Chemistry A European J

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“…extract whereby the negative control (DMSO) and extract at concentration of 0-200 mg/mL showed an insignificant difference of bacterial growth ranging from 8.99 ± 0.18 to 9.73 ± 0.05 log 10 CFU/mL. Similarly, Ugurlu et al 35 have also reported that concentrations up to 4 mmol/L had no effect on the growth of P. aeruginosa has shown an inhibiting effect on the bacterium quorum sensing mechanisms. Since compounds or concentration that do not kill or inhibit the microbial growth is less likely to promote selective pressure to develop antibacterial resistance 35 , C.…”

Section: Growth Assaymentioning

confidence: 91%

“…The tubes were centrifuged at 10,000 RPM for 10 minute twice 34 . A 5 mL of the supernatant was mixed vigorously with 3 mL chloroform before discarding the aqueous phase [34][35][36][37] . The pigment was then re-extracted with 1 mL of 0.2 N HCl and the mixture was mixed vigorously to elicit a pink red solution 35,38 .…”

Section: Pyocyanin Production Assaymentioning

confidence: 99%

“…A 5 mL of the supernatant was mixed vigorously with 3 mL chloroform before discarding the aqueous phase [34][35][36][37] . The pigment was then re-extracted with 1 mL of 0.2 N HCl and the mixture was mixed vigorously to elicit a pink red solution 35,38 . The red pink solution was centrifuged at 8,000 RPM for 10 minutes and the relative pyocyanin concentration was measured using a spectrophotometer at OD 520 with 0.2 N as blank 36,38,39 .…”

Section: Pyocyanin Production Assaymentioning

confidence: 99%

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Inhibition of Pseudomonas aeruginosa Quorum Sensing by Curcuma xanthorrhiza Roxb. Extract

Othman¹,

Rukayadi²,

Radu³

2019

J Pure Appl Microbiol

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Microorganisms such as Pseudomonas aeruginosa have always been adaptable in surviving the harsh environment such as antimicrobial agents via the quorum sensing (QS) mechanism. Studies have shown that quorum sensing mechanism cases have been highly associated with foodborne illnesses. Since synthetic compounds such as azithromycin (AZM) are reported to have detrimental effects on human, using medicinal local plants have been gaining attention as an anti-quorum agent. The aim of this study was to determine the anti-quorum sensing activity of the Curcuma xanthorrhiza Roxb. extract against P. aeruginosa ATCC35554 quorum sensing system including swarming motility, pyocyanin production and biofilm formation. The results indicated that the extract required a high concentration to inhibit and kill the P. aeruginosa with minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) values of 200 and >700 mg/mL, respectively. Thus, anti-quorum sensing assays were done in concentration up to 200 mg/mL. The inhibition of quorum sensing activity of C. xanthorrhiza Roxb. extract on P. aeruginosa quorum sensing was concentration dependent manner. At 200 mg/mL of the extract exhibited 72.12% reduction of swarming motility, 84.30% inhibition of the pyocyanin production and 78.35% reduction in the biofilm formation. In conclusion the crude extract of C. xanthorrhiza Roxb. extract has ability to reduce the virulence factors; swarming motility, pyocyanin production and biofilm formation regulated by quorum sensing. Thus, the extract C. xanthorrhiza Roxb. extract has anti-quorum sensing or quorum quenching activity.

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