Regulation and controlling the motility properties of Pseudomonas aeruginosa

Khan, Fazlurrahman; Pham, Dung Thuy Nguyen; Oloketuyi, Sandra Folarin; Kim, Young‐Mog

doi:10.1007/s00253-019-10201-w

Cited by 55 publications

(43 citation statements)

References 126 publications

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“…A motilidade twitching ocorre em superfícies sólidas e a única estrutura envolvida é o pili tipo IV. A motilidade bacteriana é essencial para a patogenicidade, uma vez que está envolvida na adesão, colonização, promoção do processo infeccioso, formação de biofilmes e fuga a ambientes desfavoráveis [5,6,26].…”

Section: Resultsunclassified

Pesquisa fenotípica dos fatores de virulência em Pseudomonas aeruginosa isolados de água de abastecimento público

et al. 2020

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A bactéria Pseudomonas aeruginosa está amplamente distribuída no ambiente. A pouca exigência nutricional deste microrganismo possibilita sua sobrevivência em água de abastecimento público, cuja importância para a saúde pública deve-se principalmente ao fato de causar infecções em indivíduos imunocomprometidos. O objetivo deste trabalho foi avaliar fenotipicamente os fatores de virulência de P. aeruginosa obtidos de água de abastecimento público. Um total de 19 isolados de P. aeruginosa foram analisados para determinação das motilidades (swarming, swimming e twitching), e avaliação da produção de protease alcalina, ramnolipídeos e DNase. Os experimentos foram realizados em triplicata em três ocasiões distintas. Para realizar a representação gráfica e a análise estatística foi utilizado o Software R Studio (1.2.5001). Todos os isolados foram produtores de protease alcalina com halo de proteólise acima de 8 mm. A atividade da DNase e a produção de ramnolipídeos foram observadas em 21,1% (n=4) e 84,2% (n=16), respectivamente. Os resultados mostraram uma alta diversidade no comportamento de motilidade entre os isolados de P. aeruginosa. Ao comparar os três tipos de motilidade, a maioria dos isolados (68,4%) apresentou produção significativa (p0,05) de motilidade swimming. Conclui-se que os isolados de P. aeruginosa testados foram capazes de produzir fatores de virulência como motilidade swimming e twitching, protease e ramnolipídeos.

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Section: Resultsunclassified

Pesquisa fenotípica dos fatores de virulência em Pseudomonas aeruginosa isolados de água de abastecimento público

et al. 2020

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“…When they reach the concentration threshold, these two AHL signaling molecules bind to their receptors, LasR and RhlR, respectively, to activate the expression of regulatory genes related to biofilm formation and virulence ( Wade et al, 2005 ). Among these two AHL systems, the rhl system is involved in regulating swarming motility that participates in the early stage of biofilm establishment ( Khan et al, 2020c ), and the biosynthesis of virulence factors, such as rhamnolipid and pyocyanine ( Winzer et al, 2000 ; Daniels et al, 2004 ; Dusane et al, 2010 ). The las system controls genes encoding elastase, alkaline protease, endotoxin A and other genes related to biofilm formation ( Wilder et al, 2011 ).…”

Section: Mechanisms Of Biofilm Formation Regulated By Qsmentioning

confidence: 99%

Regulatory Mechanisms and Promising Applications of Quorum Sensing-Inhibiting Agents in Control of Bacterial Biofilm Formation

et al. 2020

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A biofilm is an assemblage of microbial cells attached to a surface and encapsulated in an extracellular polymeric substance (EPS) matrix. The formation of a biofilm is one of the important mechanisms of bacterial resistance, which not only leads to hard-to-control bacterial infections in humans and animals but also enables bacteria to be a major problem in various fields, such as food processing, wastewater treatment and metalworking. Quorum sensing (QS) is a bacterial cell-to-cell communication process that depends on the bacterial population density and is mediated by small diffusible signaling molecules called autoinducers (AIs). Bacteria use QS to regulate diverse arrays of functions, including virulence and biofilm formation. Therefore, the interference with QS by using QS inhibiting agents, including QS inhibitors (QSIs) and quorum quenching (QQ) enzymes, to reduce or even completely repress the biofilm formation of pathogenic bacteria appears to be a promising approach to control bacterial infections. In this review, we summarize the mechanisms of QS-regulating biofilm formation and QS-inhibiting agents that control bacterial biofilm formation, strategies for the discovery of new QS inhibiting agents, and the current applications of QS-inhibiting agents in several fields to provide insight into the development of effective drugs to control pathogenic bacteria.

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“…Revitalize aminoglycosides also have been used to inhibit biofilm and pathogenic bacterial infections 23 . Attenuating motility properties can be considered as highly potential for controlling biofilm formation since attachment was one of the main steps in biofilm formation 24 , 25 . Using enzymes is also a good policy for biofilm elimination because enzymes are rabidly eco-friendly and degradable 26 , 27 .…”

Section: Introductionmentioning

confidence: 99%

Prevalence of lipase producer Aspergillus niger in nuts and anti-biofilm efficacy of its crude lipase against some human pathogenic bacteria

Yassein

Hassan

Elamary

2021

Sci Rep

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Nuts are the natural source of healthy lipids, proteins, and omega-3. They are susceptible to fungal and mycotoxins contamination because of their high nutritional value. Twenty-five species comprising 12 genera were isolated from 80 samples of dried fruits and nuts using the dilution plate method. Peanut recorded the highest level of contamination followed by coconut; almond and raisin were the lowest. Aspergillus was the most prevalent genus and A.niger, was the most dominant species. The morphological identification of the selected A.niger isolates as they were detected in high frequency of occurrence was confirmed by using 18SrRNA sequence. Ochratoxin biosynthesis gene Aopks was detected in the tested isolates. Lipase production by the selected A.niger isolates was determined with enzyme activity index (EAI) ranging from 2.02 to 3.28. A.niger-26 was the highest lipase producer with enzyme activity of 0.6 ± 0.1 U/ml by the trimetric method. Lip2 gene was also detected in the tested isolates. Finally, the antibacterial and antibiofilm efficiency of crude lipase against some human pathogens was monitored. Results exhibited great antibacterial efficacy with minimum bactericidal concentration (MBC) of 20 to 40 µl/100 µl against Escherichiacoli, Pseudomonasaeruginosa, Proteusmirabilis, and Methicillin-resistant Staphylococcusaureus (MRSA). Interestingly, significant anti-biofilm efficacy with inhibition percentages of 95.3, 74.9, 77.1 and 93.6% was observed against the tested pathogens, respectively.

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Regulation and controlling the motility properties of Pseudomonas aeruginosa

Cited by 55 publications

References 126 publications

Pesquisa fenotípica dos fatores de virulência em Pseudomonas aeruginosa isolados de água de abastecimento público

Pesquisa fenotípica dos fatores de virulência em Pseudomonas aeruginosa isolados de água de abastecimento público

Regulatory Mechanisms and Promising Applications of Quorum Sensing-Inhibiting Agents in Control of Bacterial Biofilm Formation

Prevalence of lipase producer Aspergillus niger in nuts and anti-biofilm efficacy of its crude lipase against some human pathogenic bacteria

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