Inhibitors of Bacterial Swarming Behavior

Rütschlin, Sina; Böttcher, Thomas

doi:10.1002/chem.201901961

Cited by 53 publications

(45 citation statements)

References 191 publications

(300 reference statements)

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“…This is probably because the growth remained unaffected due to slightly decreased availability of AMX in swarming plates with a greater concentration of agar (0·6%). Moreover, swarming motility in E. coli is regulated by quorum‐sensing mechanisms (Rütschlin and Böttcher 2019). Thus, explaining that high cell densities might reduce the effective concentration of free antibiotic in the medium.…”

Section: Discussionmentioning

confidence: 99%

In vitro effects of sub‐inhibitory concentrations of amoxicillin on physiological responses and virulence determinants in a commensal strain of Escherichia coli

Chadha

2021

J Appl Microbiol

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Aims:The goal was to study the effects of sub-minimum inhibitory concentrations (sub-MICs) of amoxicillin (AMX) on various physiological responses and virulence determinants in a commensal strain of Escherichia coli. Materials and Results: The commensal strain was passaged under various sub-MICs of AMX and its effect on bacterial growth, motility, biofilm formation, expression of outer membrane proteins (OMPs) and cell adhesion was analysed. Bacterial growth was diminished at 1/2 and 1/4 MICs of AMX with significant reduction in growth rate. Using crystal violet (CV) assays and quantification of surface polysaccharides we observed strong biofilm formation, together with reduced swimming motility in E. coli at 1/2 MIC of AMX. Differential OMP expression upon AMX sub-MIC exposure coincided with enhanced cell adhesion to HT-29 cells in vitro. The results demonstrated that sub-MICs of AMX can stimulate unpredictable changes in commensal bacterial strains which can be a potent source for the propagation of antibiotic resistance. Conclusions:The study reports that AMX at 1/2 MIC significantly compromised bacterial growth and swimming motility, alongside inducing biofilm formation. This was also accompanied by upregulation of a single OMP which subsequently increased cell adhesion capabilities in E. coli at 1/2 MIC, thereby enhancing its colonization and survival abilities within the gut microsphere. Significance and Impact of the Study: For the first time, the effects of AMX sub-MICs on a commensal E. coli strain were described. The results corroborate on how antibiotics can act as stimulatory molecules and determine the pathogenicity of commensal bacteria in vivo that can disseminate resistance to other intestinal pathogens or microbes.

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Section: Discussionmentioning

confidence: 99%

In vitro effects of sub‐inhibitory concentrations of amoxicillin on physiological responses and virulence determinants in a commensal strain of Escherichia coli

Chadha

2021

J Appl Microbiol

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“…Swarming motility is an organized microbial movement on surfaces, dependent on extensive flagellation, cell–cell contact, and QS. This type of motility is associated with virulence and antibiotic resistance of various microorganisms, and is considered a favorable adaptation to the challenges that arise in dynamic environments, contributing to biofilm formation and infection ( Rütschlin and Böttcher, 2019 ; Carette et al, 2020 ). Interference with this phenotype is an important alternative to reduce or prevent biofilm-based infections.…”

Section: Discussionmentioning

confidence: 99%

Exploring Phenolic Compounds as Quorum Sensing Inhibitors in Foodborne Bacteria

et al. 2021

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The emergence of multidrug-resistant bacteria stimulates the search for new substitutes to traditional antimicrobial agents, especially molecules with antivirulence properties, such as those that interfere with quorum sensing (QS). This study aimed to evaluate the potential of phenolic compounds for QS inhibition in a QS biosensor strain (Chromobacterium violaceum) and three foodborne bacterial species (Aeromonas hydrophila, Salmonella enterica serovar Montevideo, and Serratia marcescens). Initially, an in silico molecular docking study was performed to select the compounds with the greatest potential for QS inhibition, using structural variants of the CviR QS regulator of C. violaceum as target. Curcumin, capsaicin, resveratrol, gallic acid, and phloridizin presented good affinity to at least four CviR structural variants. These phenolic compounds were tested for antimicrobial activity, inhibition of biofilm formation, and anti-QS activity. The antimicrobial activity when combined with kanamycin was also assessed. Curcumin, capsaicin, and resveratrol inhibited up to 50% of violacein production by C. violaceum. Biofilm formation was inhibited by resveratrol up to 80% in A. hydrophila, by capsaicin and curcumin up to 40% in S. Montevideo and by resveratrol and capsaicin up to 60% in S. marcescens. Curcumin completely inhibited swarming motility in S. marcescens. Additionally, curcumin and resveratrol increased the sensitivity of the tested bacteria to kanamycin. These results indicate that curcumin and resveratrol at concentrations as low as 6μM are potential quorum sensing inhibitors besides having antimicrobial properties at higher concentrations, encouraging applications in the food and pharmaceutical industries.

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“…In addition, swarming motility is associated with the resistance to antimicrobial agents displayed by several clinically important pathogens (Kearns, 2010). Thus, compounds that diminish swarming motility are likely to affect biofilm formation and virulence factor production (Corral et al, 2020;Rütschlin and Böttcher, 2020). Similarly, swimming motility is also involved in the initial phase of the infection process (Kumar et al, 2018; FIGURE 6 | Indole (IND) and selected indole derivatives [3-indoleacetonitrile (I3A), 5-fluoroindole (5FI), 6-fluoroindole (6FI), 5-fluoro-2-methylindole (5F2MI), 5-methylindole (5MI), and 7-methylindole (7MI)] inhibited QS controlled violacein pigment production in Chromobacterium violaceum CV026.…”

Section: Discussionmentioning

confidence: 99%

Antibiofilm and Antivirulence Properties of Indoles Against Serratia marcescens

et al. 2020

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Indole and its derivatives have been shown to interfere with the quorum sensing (QS) systems of a wide range of bacterial pathogens. While indole has been previously shown to inhibit QS in Serratia marcescens, the effects of various indole derivatives on QS, biofilm formation, and virulence of S. marcescens remain unexplored. Hence, in the present study, we investigated the effects of 51 indole derivatives on S. marcescens biofilm formation, QS, and virulence factor production. The results obtained revealed that several indole derivatives (3-indoleacetonitrile, 5-fluoroindole, 6-fluoroindole, 7-fluoroindole, 7-methylindole, 7-nitroindole, 5-iodoindole, 5-fluoro-2methylindole, 2-methylindole-3-carboxaldehyde, and 5-methylindole) dose-dependently interfered with quorum sensing (QS) and suppressed prodigiosin production, biofilm formation, swimming motility, and swarming motility. Further assays showed 6fluoroindole and 7-methylindole suppressed fimbria-mediated yeast agglutination, extracellular polymeric substance production, and secretions of virulence factors (e.g., proteases and lipases). QS assays on Chromobacterium violaceum CV026 confirmed that indole derivatives interfered with QS. The current results demonstrate the antibiofilm and antivirulence properties of indole derivatives and their potentials in applications targeting S. marcescens virulence.

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Inhibitors of Bacterial Swarming Behavior

Cited by 53 publications

References 191 publications

In vitro effects of sub‐inhibitory concentrations of amoxicillin on physiological responses and virulence determinants in a commensal strain of Escherichia coli

In vitro effects of sub‐inhibitory concentrations of amoxicillin on physiological responses and virulence determinants in a commensal strain of Escherichia coli

Exploring Phenolic Compounds as Quorum Sensing Inhibitors in Foodborne Bacteria

Antibiofilm and Antivirulence Properties of Indoles Against Serratia marcescens

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