Phase Transition Temperature and Chilling Sensitivity of Bovine Oocytes

Arav, Amir; Zeron, Yoel; Leslie, Samuel; Behboodi, E.; Anderson, G. B.; Crowe, John H.

doi:10.1006/cryo.1996.0062

Cited by 190 publications

(103 citation statements)

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“…The osmotic stress during dehydration and rehydration may also account for cryodamage [32]. To overcome extreme sensitivity to chilling temperatures, vitrification replaced classical freezing which results in increased morphological damage to oocytes at any maturation stage [33] and reduced embryonic development compared to vitrified oocytes [34].…”

Section: Discussionmentioning

confidence: 99%

“…Cryopreservation of immature oocytes could provide a partial solution for damage inflicted to cytoskeleton by vitrification, as oocytes do not contain polymerized tubules at this stage. However, oocytes at the GV stage are extremely sensitive to chilling temperatures, with the damage occurring at the cytoplasmic membrane level [33].…”

Section: Discussionmentioning

confidence: 99%

“…In addition, ZP may be either broken or at least modified by the premature release of CG, as seen in frozen-thawed human and mouse oocytes [44,54]. Chilling can induce irreversible phase changes of lipid bilayers and even membrane lysis in bovine oocyte membranes [33,55]. Modifications to the structure of cytoskeleton, mitochondria, CG and nucleoli [53] and smooth endoplasmic reticulum [15] have also been observed in vitrified-warmed bovine oocytes.…”

Section: Discussionmentioning

confidence: 99%

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Bovine oocyte vitrification before or after meiotic arrest: effects on ultrastructure and developmental ability

et al. 2005

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The nuclear stage at which oocytes are cryopreserved influences further development ability and cryopreservation affects ultrastructure of both cumulus cells and the oocyte. In this work, we analyze the effects of vitrification at different nuclear and cytoplasmic maturation stages on the oocyte ultrastructure and developmental ability. Culture in TCM199 + PVA with roscovitine 25 M during 24 h led to meiotic arrest (MA) in cumulus-oocyte complexes (COCs), while permissive in vitro maturation (IVM) was performed in TCM199, 10% FCS, FSH-LH and 17b-estradiol for 24 h. Oocytes were vitrified using the open pulled straw method (OPS) with minor modifications. Fresh and vitrified/warmed COCs were fixed as immature, after IVM, after meiotic arrest (MA) and after MA + IVM.Vitrification combined with MA followed by IVM produced the highest rates of degeneration, regardless of the vitrification time. As a consequence, lower proportions of embryos cleaved in these groups, although differences were eliminated at the five-eight cell stage. Development rates up to day 8 were similar in all experimental groups, being significantly lower than those in fresh controls. Only oocytes vitrified after IVM were able to give blastociysts. The morphological alterations observed can be responsible for compromised development. More research is needed to explain the low survival rates of the bovine oocyte after vitrification and warming. #

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Bovine oocyte vitrification before or after meiotic arrest: effects on ultrastructure and developmental ability

et al. 2005

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“…The volume of the mammalian oocyte is in the range of three to four orders of magnitude larger than that of the spermatozoa, thus substantially decreasing the surface-to-volume ratio and making them very sensitive to chilling and highly susceptible to intracellular ice formation (Ruffing et al, 1993, Arav et al, 1996. This problem becomes even more pronounced in non-mammalian vertebrates (fish, birds, amphibians, and reptiles) whose oocytes are considerably larger than those of mammals (Guenther et al, 2006).…”

Section: Factors Affecting Oocyte Freezing and Resolutionmentioning

confidence: 99%

Fertility Cryopreservation

Ciani¹,

Cocchia²,

Esposito³

et al. 2012

Advances in Embryo Transfer

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“…Additionaly, failure of the meiotic spindles could lead to chromosomal dispersion and high incidence of aneuploidy or polyploidy [8,11]. Some studies have described irreversible structural damage to the oocyte membrane or impaired intercellular communication between the oocyte and the cumulus cells after cryopreservation of germinal vesicle (GV) oocytes [12]. Therefore, choosing an intermediate stage, such as germinal vesicle breakdown (GVBD), may circumvent some of the problems associated with the cryopreservation of GV and MII oocytes.…”

Section: Introductionmentioning

confidence: 99%

Mouse oocyte vitrification: the effects of two methods on maturing germinal vesicle breakdown oocytes

Khosravi-Farsani

Sobhani

Amidi

et al. 2010

J Assist Reprod Genet

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Purpose Evaluation of viability and subsequent developmental ability of mouse germinal vesicle breakdown oocytes vitrified in conventional straws. Methods Oocytes with compact cumulus cells were cultured for 3 h in TCM199 medium GVBD and vitrified by two methods: the step-wise and single-step. After vitrification, the oocytes were thawed, and subjected to in vitro maturation and in vitro fertilization. Oocyte survival (postthaw) was assessed by morphological appearance and staining, using propidium iodide (PI)/Hoechst 33342. The oocyte maturation and fertilization rates were examined in vitro. Results In the single-step method the rates of post thaw survival, maturation to metaphase II and cleavage (2-cell embryos) were 58.68%, 56.41% and 38.63%, respectively. In the step-wise method, the corresponding rates were 81.75%, 68.59% and 51.80%, respectively. Conclusion Vitrification of mouse germinal vesicle breakdown oocytes by the step-wise method had the advantage of maintaining the viability and subsequent production of 2-cell embryos. In comparison with that in unvitrified control oocytes, the development of MII oocytes to 2-cell embryos was impaired following vitrification.

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Phase Transition Temperature and Chilling Sensitivity of Bovine Oocytes

Cited by 190 publications

References 0 publications

Bovine oocyte vitrification before or after meiotic arrest: effects on ultrastructure and developmental ability

Bovine oocyte vitrification before or after meiotic arrest: effects on ultrastructure and developmental ability

Fertility Cryopreservation

Mouse oocyte vitrification: the effects of two methods on maturing germinal vesicle breakdown oocytes

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