Phase contrast radiography of Lewy bodies in Parkinson disease

Koh, Seong-Beom; Suh, Sangil; Lee, D. H.; Kim, A. R.; Oh, Cheol; Yoon, Joon Shik; Weon, Byung Mook; Seol, Seung Kwon; Pyun, Ahram; Je, Jung Ho; Hwu, Y.; Margaritondo, G.

doi:10.1016/j.neuroimage.2006.04.217

Cited by 14 publications

(15 citation statements)

References 17 publications

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“…Deposits in brain system with complex microscopic geometry could profit from the performance of this approach. This specifically applied to the studies of Lewy bodies39 and amyloid plaques40 in human brains. The current study on acervuli, another type of deposits in brain system, is promising as well.…”

Section: Discussionmentioning

confidence: 99%

Growth patterns for acervuli in human pineal gland

Kim

Chang

et al. 2012

Sci Rep

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Acervuli are calcified concretions in the pineal gland (PG). Particularly interesting are their incidence and size, which are believed to affect neurological disorders and many physiological functions of PG such as regulating circadian rhythm. Despite long investigations for a century, detailed growth mechanism of acervuli has yet to be studied. Here we study the growth morphology of acervuli in human PGs by a direct visualization in 3-dimension (3-D) using a synchrotron X-ray imaging method. For an entire PG, non-aggregated acervuli show Gaussian distribution in size with 47±28 µm. The 3-D volume rendered images of acervuli reveal that the bumpy surfaces developed by lamination result in the mulberry-like structure. In addition, coalescence of multiple acervuli leads to large-scale lamination on the whole aggregate. We suggest a novel hypothesis on the growth patterns of acervuli by their nucleation density (Nd): i) mulberry-like structure at low Nd, and ii) large-scale lamination on an aggregate at high Nd.

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Section: Discussionmentioning

confidence: 99%

Growth patterns for acervuli in human pineal gland

Kim

Chang

et al. 2012

Sci Rep

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“…6 Hence, it is expected that PCR would be applied in many of future studies due to its potential applications in live specimens without any dissection of tissue.…”

Section: Discussionmentioning

confidence: 99%

“…According to the previous protocol, the detector system consisted of a single, thin (150 mm), cleaved crystal of CdWO 4 as the scintillator and a CCD camera. 6 A microscopic objective lens magnified the image displayed on the scintillator before it was captured by the CCD according to the same previous protocol (Fig. 1).…”

Section: Methodsmentioning

confidence: 99%

Feasibility of phase contrast radiography in cat normal spinal cord

Yoon¹,

Kim²,

Kim³

et al. 2008

The Imaging Science Journal

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Phase contrast radiography (PCR) techniques are becoming important analytical tools for imaging biological samples. The authors aimed to evaluate a possible use of PCR in examining radiological microscopic findings of cat normal spinal cord without any staining procedure in paraffin blocked specimens. The PCR experiments were implemented at the International Consortium of Phase Contrast Imaging and Radiology (ICPCIR) (7B2) beamline at the Pohang Light Source in Korea. In the phase contrast radiography, the grey and white matters of the spinal cord were demarcated with the grey matter in an 'H' shape, similar to the histological structure by light microscopy. The dorsal, intermediate and ventral grey matters were clearly visible as well as other columns and funiculi. The authors suggest that PCR could be a useful tool for microscopic morphological investigation of spinal cord without any staining procedure.

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“…1 PCXM has many advantages over conventional radiography, including good spatial resolution better than 1 µm and realtime resolution radiography . [4][5][6][7] Recently, X-ray imaging technology based on phase-contrast mechanisms has greatly advanced, thanks to the use of unmonochromatized synchrotron hard X-rays for biological specimens. 5 The high intensity, tunable energy and polarization of the synchrotron radiation make it an ideal X-ray source for many applications in the medical science .…”

Section: Introductionmentioning

confidence: 99%

“…5 The high intensity, tunable energy and polarization of the synchrotron radiation make it an ideal X-ray source for many applications in the medical science . [1][2][3][4][5] We investigated a possibility of PCXM using an unmonochromatized synchrotron radiation source to become a new technique for the early non-invasive diagnosis of athelosclerosis, ranal cell carcinoma, prostatic cancer, parkinson's disease, crack tooth syndrome and basal cell carcinoma (BCC).…”

Section: Introductionmentioning

confidence: 99%

Various clinical application of phase contrast x-ray

Park

et al. 2008

SPIE Proceedings

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In biomedical application study using phase contrast X-ray, both sample thickness or density and absorption difference are very important factors in aspects of contrast enhancement. We present experimental evidence that synchrotron hard X-ray are suitable for radiological imaging of biological samples down to the cellular level. We investigated the potential of refractive index radiology using un-monochromatized synchrotron hard X-rays for the imaging of cell and tissue in various diseases. Material had been adopted various medical field, such as apoE knockout mouse in cardiologic field, specimen from renal and prostatic carcinoma patient in urology, basal cell epithelioma in dermatology, brain tissue from autosy sample of pakinson's disease, artificially induced artilrtis tissue from rabbits and extracted tooth from patients of crack tooth syndrome. Formalin and paraffin fixed tissue blocks were cut in 3 ㎜ thickness for the X-ray radiographic imaging.From adjacent areas, 4 ㎛ thickness sections were also prepared for hematoxylin-eosin staining. Radiographic images of dissected tissues were obtained using the hard X-rays from the 7B2 beamline of the Pohang Light Source (PLS). The technique used for the study was the phase contrast images were compared with the optical microscopic images of corresponding histological slides. Radiographic images of various diseased tissues showed clear histological details of organelles in normal tissues. Most of cancerous lesions were well differentiated from adjacent normal tissues and detailed histological features of each tumor were clearly identified. Also normal microstructures were identifiable by the phase contrast imaging. Tissue in cancer or other disease showed clearly different findings from those of surrounding normal tissue. For the first time we successfully demonstrated that synchrotron hard X-rays can be used for radiological imaging of relatively thick tissue samples with great histological details.

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Phase contrast radiography of Lewy bodies in Parkinson disease

Cited by 14 publications

References 17 publications

Growth patterns for acervuli in human pineal gland

Growth patterns for acervuli in human pineal gland

Feasibility of phase contrast radiography in cat normal spinal cord

Various clinical application of phase contrast x-ray

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