Pharmacological Prevention of Ocular Inflammation Induced by Lens Proteins

Abstract

Ocular inflammation was induced by lens protein to minic the traumatic injury of the eyes. Pretreatment of the eyes with indomethacin resulted in marked reduction of this ocular inflammation in the early phase. Pretreatment of the eyes with phenidone and nordihydroguaiaretic acid (NDGA) on the other hand reduced ocular inflammation during both early and late phases. These results indicate that prostaglandins are involved in early phase of the inflammation which can be reduced with cyclooxygenase inhibitors such as indomethacin and leukotrienes are responsible primarily in the later phase because they are suppressed by lipoxygenase inhibitors such as phenidone and NDGA. Although histamine is implicated in the process of inflammation, the H₁-antagonist, pyrilamine, had very little effect on ocular inflammation, whereas the H₂-antagonist, cimetidine, exhibited no effect but rather enhanced the ocular inflammatory responses at the peak of inflammation. These results are not in accord with published findings.

Ocular Anti-Inflammatory Actions of Matrine

“…Ocular inflammation was induced by the administration of lens proteins into the anterior chamber of albino rabbit eyes and graded with the method reported previously (6). Briefly, 25 yl of rabbit lens protein solution containing 23.72 mg/ml of protein was injected into anterior chamber of the rabbit eye.…”

“…Briefly, 25 yl of rabbit lens protein solution containing 23.72 mg/ml of protein was injected into anterior chamber of the rabbit eye. Ocular inflammation was measured with a slit lamp biomicroscope (Marco, Jacksonville, FL) at 1/6, 1/2, 1, 2, 3, 4, 5, 24 and 48 hr after lens protein injection (6). Animals were treated with 50 pi of 1% matrine topically or with vehicle 1 hr before lens protein injection.…”

Inhibitory Effect of Tetrandrine on Lens Proteins-Induced Ocular Inflammation in Rabbits

“…Lens proteins was prepared according to the methods of Migano and Chiou (5). The eye lens of four Albino rabbits were isolated and homogenized (10% W/V) in Hank's balanced salt solution (HBSS).…”

“…But blocking the cyclooxygenase arm of the AA cascade potentiates the production of lipoxygenase metabolites, leukotrienes, which are responsible for the late phase of inflammation and the chemotoxis of leukocytes (5). These results indicate that NSAID have the potential of worsening the inflammation , especially during the late phase of the inflammation.…”

Effects of Sodium Naproxen Eye Drops on Rabbit Ocular Inflammation Induced by Sodium Arachidonate

“…Numerous investigations have demonstrated that cyclooxygenase and lipoxygenase products from AA are synthetized in inflamed ocular tissues (3)(4)(5)(6) and reproduce many of the characteristic signs of inflammation when applied either topically or intracamerally (7,8). Moreover, it has been shown that several nonsteroidal anti-inflammatory drugs (NSAIDs) may reduce the cardinal signs of ocular inflammation by inhibition of cyclooxygenase enzyme which converts AA into PGs and thromboxanes (9)(10)(11)(12)(13)(14)(15).…”