zebrafish embryo, the intracellular ROS level was estimated by 5 mM H 2 O 2 stress in the zebrafish embryo, and inhibition of apoptosis was evaluated. The antioxidant enzymes were extracted from the H 2 O 2 -stressed zebrafish embryo, and the intracellular ROS was eliminated due to RM12. Collectively, the experiment showed that the substance P from the freshwater murrel C. striatus possessed potent antioxidant properties; thus, it can further be focused to develop it as antioxidant molecule in aquaculture organisms.
KeywordsSubstance P • Tachykinin • Channa striatus • Antioxidant mechanism • Intracellular ROS Abbreviations ROS Reactive oxygen species H 2 O 2 Hydrogen peroxide ESR Electron spin resonance DPPH 2,2-Diphenyl-1-picryl-hydrazyl-hydrate ABTS 2,2-Azinobis 3-ethylbenzothiazoline-6-sulfonic acid SOAH Superoxide anion radical scavenging assay HORS Hydroxyl radical scavenging assay DCFH-DA 2′-7′-Dichlorodihydrofluorescein diacetate TEAC Trolox equivalent antioxidant capacity PMS Phenazine methosulphate NADH Nicotinamide adenine dinucleotide NBT Nitro blue tetrazolium