Antibacterial Activity of RM12, a Tachykinin Derivative, Against Pseudomonas aeruginosa

Raju, Stefi V.; Sarkar, Purabi; Pasupuleti, Mukesh; Abbasi, Arshad Mehmood; Al-Farraj, Dunia A.; Elshikh, Mohamed S.; Elumalai, Preetham; Harikrishnan, Ramasamy; Rahman, MM; Arockiaraj, Jesu

doi:10.1007/s10989-021-10274-0

Cited by 6 publications

(7 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The FESEM analysis showed the effect of GE18 to significantly damage the cell membrane. Similar to this effect by GE18, RM12 peptide has been reported to damage the cell membrane of P. aeruginosa [ 36 ].…”

Section: Discussionmentioning

confidence: 60%

“…Significant anti-bacterial activity was found against P. aeruginosa in the radial diffusion and MIC assays. This anti-microbial effect of GE18 is similar to the effect of RM12, a peptide from the Tachykinin protein derivative of Channa striatus [ 36 ]. The bactericidal effect of peptides may be promoted by the bacterial membrane damage caused by cell membrane integrity, structural disturbance, transport into the cytoplasm and adhesion to intercellular receptor molecules [ 63 ].…”

Section: Discussionmentioning

confidence: 84%

“…According to the results of the radial diffusion assay, further experiments were carried out using P. aeruginosa . To determine the minimum inhibitory concentration (MIC), a broth micro-dilution assay was performed [ 36 ]. Briefly, the 16 h log phase culture of P. aeruginosa was centrifuged.…”

Section: Methodsmentioning

confidence: 99%

“…The biofilm inhibition assay measured the peptide’s efficiency to prevent biofilm maturation [ 36 ]. Briefly, the log phase P. aeruginosa culture, at a concentration of 1 × 10 8 cells/mL, was inoculated in a 96-well plate with a 200 μL culture volume.…”

Section: Methodsmentioning

confidence: 99%

“…The effect of GE18 on bacterial cellular morphology was examined through a field-emission scanning electron microscopy (FESEM) analysis [ 36 ]. The log phase culture was treated with GE18 for 2 h, the untreated group served as the control and 1 mg/mL of the gentamicin treatment group was considered the positive control.…”

Section: Methodsmentioning

confidence: 99%

See 4 more Smart Citations

Aquatic Peptide: The Potential Anti-Cancer and Anti-Microbial Activity of GE18 Derived from Pathogenic Fungus Aphanomyces invadans

Velayutham,

Priya,

Sarkar

et al. 2023

Molecules

Self Cite

View full text Add to dashboard Cite

Small molecules as well as peptide-based therapeutic approaches have attracted global interest due to their lower or no toxicity in nature, and their potential in addressing several health complications including immune diseases, cardiovascular diseases, metabolic disorders, osteoporosis and cancer. This study proposed a peptide, GE18 of subtilisin-like peptidase from the virulence factor of aquatic pathogenic fungus Aphanomyces invadans, which elicits anti-cancer and anti-microbial activities. To understand the potential GE18 peptide-induced biological effects, an in silico analysis, in vitro (L6 cells) and in vivo toxicity assays (using zebrafish embryo), in vitro anti-cancer assays and anti-microbial assays were performed. The outcomes of the in silico analyses demonstrated that the GE18 peptide has potent anti-cancer and anti-microbial activities. GE18 is non-toxic to in vitro non-cancerous cells and in vivo zebrafish larvae. However, the peptide showed significant anti-cancer properties against MCF-7 cells with an IC50 value of 35.34 µM, at 24 h. Besides the anti-proliferative effect on cancer cells, the peptide exposure does promote the ROS concentration, mitochondrial membrane potential and the subsequent upregulation of anti-cancer genes. On the other hand, GE18 elicits significant anti-microbial activity against P. aeruginosa, wherein GE18 significantly inhibits bacterial biofilm formation. Since the peptide has positively charged amino acid residues, it targets the cell membrane, as is evident in the FESEM analysis. Based on these outcomes, it is possible that the GE18 peptide is a significant anti-cancer and anti-microbial molecule.

show abstract

Section: Discussionmentioning

confidence: 60%

Section: Discussionmentioning

confidence: 84%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations