RM12 similar to substance P from tachykinin of freshwater murrel Channa striatus influence intracellular ROS in vitro fish erythrocytes and developmental toxicity and antioxidant enzymes in vivo zebrafish embryo

Raju, Stefi V.; Mukherjee, Arnab; Sarkar, Purabi; Issac, Praveen Kumar; Lite, Christy; Paray, Bilal Ahmad; Khalid, Muhammad; Al-Mfarij, Abdul Rahman; Arockiaraj, Jesu

doi:10.1007/s10695-021-00950-9

Cited by 8 publications

(3 citation statements)

References 42 publications

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“…Somite should be formed at least at 48 hpf, failing which the embryo was considered dead. Mortality was recorded till 96 hpf ( t = 24, 48, 72, and 96 hpf, n = 40/group, three independent experiments) 42 …”

Section: Methodsmentioning

confidence: 99%

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Embryonic exposure to butylparaben and propylparaben induced developmental toxicity and triggered anxiety‐like neurobehavioral response associated with oxidative stress and apoptosis in the head of zebrafish larvae

Lite

Guru

Juliet

et al. 2022

Environmental Toxicology

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Parabens are synthetic antimicrobial compounds used as a preservative for extending the shelf life of food, pharmaceutical and cosmetic products. The alkyl chain length of the paraben esters positively correlates with their antimicrobial property. Hence, long-chain paraben esters, namely butylparaben and propylparaben, are used in combination as they have better solubility and antimicrobial efficacy. Extensive use of parabens has now resulted in the ubiquitous presence of these compounds in various human and environmental matrices. During early life, exposure to environmental contaminants is known to cause oxidative-stress mediated apoptosis in developing organs. The brain being one of the high oxygen-consuming, metabolically active and lipid-rich organ, it is primarily susceptible to reactive oxygen species (ROS) and lipid peroxidation (LP) induced neuronal cell death. The primary cause for the impairment in cognitive and emotional neurobehvioural outcomes in neurodegenerative disease was found to be associated with neuronal apoptosis. The present study aimed to study butylparaben and propylparaben's effect on zebrafish during early embryonic stages. Besides this, the association between alteration in anxiety-like neurobehavioral response with oxidative stress and antioxidant status in head region was also studied. The study results showed variation in the toxic signature left by butylparaben and propylparaben on developmental parameters such as hatching rate, survival and non-lethal malformations in a time-dependent manner. Data from the light-dark preference test showed embryonic exposure to butylparaben and propylparaben to trigger anxiety-like behavior in zebrafish larvae. In addition, a significant increase in intracellular ROS and LP levels correlated with suppressed antioxidant enzymes: superoxide dismutases (SOD), catalases (CAT), Glutathione peroxidase (GPx), glutathione S-transferase (GST), and Glutathione (GSH) activity in the head region of the zebrafish larvae. Acetylcholinesterase (AChE) activity was also suppressed in the exposed groups, along with increased nitric oxide production. The overall observations show increased oxidative stress indices correlating with upregulated expression of apoptotic cells in a dose-dependent manner. Collectively, our findings reveal butylparaben and propylparaben as an anxiogenic neuroactive Christy Lite, Ajay Guru, and Melita Juliet contributed equally to this study.

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Section: Methodsmentioning

confidence: 99%

“…Mortality was recorded till 96 hpf (t = 24, 48, 72, and 96 hpf, n = 40/group, three independent experiments). 42…”

Section: Mortalitymentioning

confidence: 99%

Embryonic exposure to butylparaben and propylparaben induced developmental toxicity and triggered anxiety‐like neurobehavioral response associated with oxidative stress and apoptosis in the head of zebrafish larvae

Lite

Guru

Juliet

et al. 2022

Environmental Toxicology

Self Cite

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“…Approximately 20 articles from Google Scholar and 15 articles from PubMed were deemed relevant to our investigation. Notably, the most frequently cited papers included one by [14] with 28 citations, highlighting the disruption of zebrafish larval microbiomes and increased mortality induced by Streptomycin exposure. Another study by [15], with 6 citations, reported elevated mortality and skeletal deformities in zebrafish exposed to MS-222 during early development.…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of 2-Methylimidazole and 4-Methylimidazole Exposure Effects on Zebrafish Larvae Mortality Rate

Riyaz,

Praveen Kumar

2024

E3S Web Conf.

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The study aimed to compare zebrafish embryo mortality rates under different 2-Methylimidazole (2-MI) and 4-Methylimidazole (4-MI) concentrations. Two exposure groups were created at a 40μM concentration using egg water. Thirty individuals were divided into two groups: Group 1 received an environmentally relevant dose of 2-MI, while Group 2 was exposed to 4-MI. Each group’s embryos (n=15 per plate) were observed from 4 to 96 hours post fertilization (hpf), adhering to OECD guidelines. Statistical analysis using SPSS maintained a 95% confidence level, 0.01 ratio, 80% power, and 1:1 enrollment ratio. Results showed a marked increase in mortality rate (71.87±1.25) in ecologically exposed embryos compared to 2-MI (45.73±0.79), with a significant P-value of 0.000 (P<0.05). Additionally, zebrafish exposed to 4-MI displayed a 20% lower mortality rate than 2-MI, supported by a significance threshold of p = 0.000 (p<0.05). These findings highlight the detrimental impact of early exposure to 2-MI and 4-MI on zebrafish embryo mortality rates, signifying developmental and embryotoxic implications.

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Textile azo dye, Sudan Black B, inducing hepatotoxicity demonstrated in in vivo zebrafish larval model

Ramamurthy,

Madesh,

Priya

et al. 2024

Fish Physiol Biochem

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RM12 similar to substance P from tachykinin of freshwater murrel Channa striatus influence intracellular ROS in vitro fish erythrocytes and developmental toxicity and antioxidant enzymes in vivo zebrafish embryo

Cited by 8 publications

References 42 publications

Embryonic exposure to butylparaben and propylparaben induced developmental toxicity and triggered anxiety‐like neurobehavioral response associated with oxidative stress and apoptosis in the head of zebrafish larvae

Embryonic exposure to butylparaben and propylparaben induced developmental toxicity and triggered anxiety‐like neurobehavioral response associated with oxidative stress and apoptosis in the head of zebrafish larvae

Comparative Analysis of 2-Methylimidazole and 4-Methylimidazole Exposure Effects on Zebrafish Larvae Mortality Rate

Textile azo dye, Sudan Black B, inducing hepatotoxicity demonstrated in in vivo zebrafish larval model

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